The electrokinetic surface of five enteropathogenic Escherichia coli serogroups

Detalhes bibliográficos
Autor(a) principal: Mangia, Adriana Hamond Regua
Data de Publicação: 1995
Outros Autores: Teixeira, Lúcia Martins, Costa e Silva Filho, Fernando
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/27119
Resumo: Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil
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spelling Mangia, Adriana Hamond ReguaTeixeira, Lúcia MartinsCosta e Silva Filho, Fernando2018-06-26T16:37:10Z2018-06-26T16:37:10Z1995MANGIA, Adriana Hamond Regua; TEIXEIRA, Lúcia Martins; COSTA E SILVA FILHO, Fernando. The electrokinetic surface of five enteropathogenic Escherichia coli serogroups. Cell Biochemistry and Biophysics, v.26, n.1, p.45-55, 1995.1085-9195https://www.arca.fiocruz.br/handle/icict/271191559-0283engSpringer International PublishingEscherichia coli EnteropatogênicaEscherichia coliEPECCell SurfaceSurface ChargeEnteropathogenic Escherichia coliThe electrokinetic surface of five enteropathogenic Escherichia coli serogroupsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Microbiologia. Rio de Janeiro, RJ, BrasilUniversidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, BrasilThe surface ionogenicity of five enteropathogenicEscherichia coli serogroups (O111:H2, O111:H12, O125:H9, O119:H6, and O26:H11) was investigated by electrokinetical approaches. All of the studied surfaces are negatively charged with their mean values of zeta potential (ZP) varying from −9.0 (O26:H11) to −11.9 mV (O111:H2). The populational behavior of the all bacteria are similar since very high ZP values varying from −26 to −30 mV were obtained in experiments carried out with the slip plane calculated at 6.83 nm from the cell surface. All the surfaces are extremely acidic, because the isoelectrophoretic points are localized at pH values below 3.0. Treatment of the microorganisms with neuraminidase did not alter their surface anionogenicity, while treatment with trypsin or phospholipase C reduced their negative charge.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/27119/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALThe electrokinetic surface...Regua mangia.pdfapplication/pdf740417https://www.arca.fiocruz.br/bitstream/icict/27119/2/The%20electrokinetic%20surface...Regua%20mangia.pdf4ba556c2085353aa56397c6f15ba5628MD52TEXTThe electrokinetic surface...Regua mangia.pdf.txtThe electrokinetic surface...Regua mangia.pdf.txtExtracted texttext/plain21465https://www.arca.fiocruz.br/bitstream/icict/27119/3/The%20electrokinetic%20surface...Regua%20mangia.pdf.txt1307c504317eb376064dd00db9867441MD53icict/271192023-01-13 11:36:46.784oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-01-13T14:36:46Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv The electrokinetic surface of five enteropathogenic Escherichia coli serogroups
title The electrokinetic surface of five enteropathogenic Escherichia coli serogroups
spellingShingle The electrokinetic surface of five enteropathogenic Escherichia coli serogroups
Mangia, Adriana Hamond Regua
Escherichia coli Enteropatogênica
Escherichia coli
EPEC
Cell Surface
Surface Charge
Enteropathogenic Escherichia coli
title_short The electrokinetic surface of five enteropathogenic Escherichia coli serogroups
title_full The electrokinetic surface of five enteropathogenic Escherichia coli serogroups
title_fullStr The electrokinetic surface of five enteropathogenic Escherichia coli serogroups
title_full_unstemmed The electrokinetic surface of five enteropathogenic Escherichia coli serogroups
title_sort The electrokinetic surface of five enteropathogenic Escherichia coli serogroups
author Mangia, Adriana Hamond Regua
author_facet Mangia, Adriana Hamond Regua
Teixeira, Lúcia Martins
Costa e Silva Filho, Fernando
author_role author
author2 Teixeira, Lúcia Martins
Costa e Silva Filho, Fernando
author2_role author
author
dc.contributor.author.fl_str_mv Mangia, Adriana Hamond Regua
Teixeira, Lúcia Martins
Costa e Silva Filho, Fernando
dc.subject.other.pt_BR.fl_str_mv Escherichia coli Enteropatogênica
topic Escherichia coli Enteropatogênica
Escherichia coli
EPEC
Cell Surface
Surface Charge
Enteropathogenic Escherichia coli
dc.subject.en.pt_BR.fl_str_mv Escherichia coli
EPEC
Cell Surface
Surface Charge
dc.subject.decs.pt_BR.fl_str_mv Enteropathogenic Escherichia coli
description Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil
publishDate 1995
dc.date.issued.fl_str_mv 1995
dc.date.accessioned.fl_str_mv 2018-06-26T16:37:10Z
dc.date.available.fl_str_mv 2018-06-26T16:37:10Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MANGIA, Adriana Hamond Regua; TEIXEIRA, Lúcia Martins; COSTA E SILVA FILHO, Fernando. The electrokinetic surface of five enteropathogenic Escherichia coli serogroups. Cell Biochemistry and Biophysics, v.26, n.1, p.45-55, 1995.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/27119
dc.identifier.issn.pt_BR.fl_str_mv 1085-9195
dc.identifier.eissn.none.fl_str_mv 1559-0283
identifier_str_mv MANGIA, Adriana Hamond Regua; TEIXEIRA, Lúcia Martins; COSTA E SILVA FILHO, Fernando. The electrokinetic surface of five enteropathogenic Escherichia coli serogroups. Cell Biochemistry and Biophysics, v.26, n.1, p.45-55, 1995.
1085-9195
1559-0283
url https://www.arca.fiocruz.br/handle/icict/27119
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer International Publishing
publisher.none.fl_str_mv Springer International Publishing
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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