A preliminary investigation on the chemical composition of the cell surface of five enteropathogenic Escherichia coli serotypes

Detalhes bibliográficos
Autor(a) principal: Mangia, Adriana Hamond Regua
Data de Publicação: 1999
Outros Autores: Bergter, Eliana Barreto, Teixeira, Lúcia Martins, Costa e Silva Filho, Fernando
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/25682
Resumo: Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil
id CRUZ_fa12e4be6d98fdaaace9b44395318dc1
oai_identifier_str oai:www.arca.fiocruz.br:icict/25682
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Mangia, Adriana Hamond ReguaBergter, Eliana BarretoTeixeira, Lúcia MartinsCosta e Silva Filho, Fernando2018-04-09T14:27:11Z2018-04-09T14:27:11Z1999MANGIA, Adriana Hamond Regua et al. A preliminary investigation on the chemical composition of the cell surface of five enteropathogenic Escherichia coli serotypes. Memórias do Instituto Oswaldo Cruz, v.94, n.4, p.513-518,19990074-0276https://www.arca.fiocruz.br/handle/icict/2568210.1590/S0074-027619990004000161678-8060engIOC/FiocruzA preliminary investigation on the chemical composition of the cell surface of five enteropathogenic Escherichia coli serotypesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Microbiologia. Rio de Janeiro, RJ, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Microbiologia. Rio de Janeiro, RJ, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, BrasilThe cell surfaces of five enteropathogenic Escherichia coli serotypes (O111:H2; O111:H12; O125:H9; O119:H6; O26:H11) were assayed by chemical methods, lectin agglutination tests and spectroscopy associated to transmission electron microscopy. Results of lectin agglutination assays showed that all strains reacted with mannosebinding lectins. Strains belonging to serotype O125:H9 also agglutinated with lectins which recognize galactose and Nacetylgalactosamine residues. The bacterial cells were treated with 0.01M phosphate buffered saline (pH 7.0) at 100oC for 2 hr and the extracts were submitted to precipitation and fractionated by Cetavlon. Phosphate, total sugar and protein contents were determined. Gas liquid chomatography-mass spectrometry analysis of alditol acetates showed the presence of galactose, mannose, fucose, glucose and traces of ribose. Spectroscopic analysis of intact cells showed the presence of a capsule-like structure which was not totally preserved after extraction. Some cells were still surrounded by an amorphous capsular-like material after polysaccharide extraction.Enteropathogenic Escherichia ColiCell SurfaceLectinsEscherichia Coliinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/25682/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALA preliminary investigation... Regua Mangia.pdfapplication/pdf300354https://www.arca.fiocruz.br/bitstream/icict/25682/2/A%20preliminary%20investigation...%20Regua%20Mangia.pdfd9a62222248f647fc1faaa9ab1c3ca21MD52TEXTA preliminary investigation... Regua Mangia.pdf.txtA preliminary investigation... Regua Mangia.pdf.txtExtracted texttext/plain23083https://www.arca.fiocruz.br/bitstream/icict/25682/3/A%20preliminary%20investigation...%20Regua%20Mangia.pdf.txt45acb56c3352209a0376d29bd6e5eeedMD53icict/256822023-01-12 15:30:18.52oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-01-12T18:30:18Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv A preliminary investigation on the chemical composition of the cell surface of five enteropathogenic Escherichia coli serotypes
title A preliminary investigation on the chemical composition of the cell surface of five enteropathogenic Escherichia coli serotypes
spellingShingle A preliminary investigation on the chemical composition of the cell surface of five enteropathogenic Escherichia coli serotypes
Mangia, Adriana Hamond Regua
Enteropathogenic Escherichia Coli
Cell Surface
Lectins
Escherichia Coli
title_short A preliminary investigation on the chemical composition of the cell surface of five enteropathogenic Escherichia coli serotypes
title_full A preliminary investigation on the chemical composition of the cell surface of five enteropathogenic Escherichia coli serotypes
title_fullStr A preliminary investigation on the chemical composition of the cell surface of five enteropathogenic Escherichia coli serotypes
title_full_unstemmed A preliminary investigation on the chemical composition of the cell surface of five enteropathogenic Escherichia coli serotypes
title_sort A preliminary investigation on the chemical composition of the cell surface of five enteropathogenic Escherichia coli serotypes
author Mangia, Adriana Hamond Regua
author_facet Mangia, Adriana Hamond Regua
Bergter, Eliana Barreto
Teixeira, Lúcia Martins
Costa e Silva Filho, Fernando
author_role author
author2 Bergter, Eliana Barreto
Teixeira, Lúcia Martins
Costa e Silva Filho, Fernando
author2_role author
author
author
dc.contributor.author.fl_str_mv Mangia, Adriana Hamond Regua
Bergter, Eliana Barreto
Teixeira, Lúcia Martins
Costa e Silva Filho, Fernando
dc.subject.en.pt_BR.fl_str_mv Enteropathogenic Escherichia Coli
Cell Surface
Lectins
topic Enteropathogenic Escherichia Coli
Cell Surface
Lectins
Escherichia Coli
dc.subject.decs.pt_BR.fl_str_mv Escherichia Coli
description Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil
publishDate 1999
dc.date.issued.fl_str_mv 1999
dc.date.accessioned.fl_str_mv 2018-04-09T14:27:11Z
dc.date.available.fl_str_mv 2018-04-09T14:27:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MANGIA, Adriana Hamond Regua et al. A preliminary investigation on the chemical composition of the cell surface of five enteropathogenic Escherichia coli serotypes. Memórias do Instituto Oswaldo Cruz, v.94, n.4, p.513-518,1999
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/25682
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02761999000400016
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv MANGIA, Adriana Hamond Regua et al. A preliminary investigation on the chemical composition of the cell surface of five enteropathogenic Escherichia coli serotypes. Memórias do Instituto Oswaldo Cruz, v.94, n.4, p.513-518,1999
0074-0276
10.1590/S0074-02761999000400016
1678-8060
url https://www.arca.fiocruz.br/handle/icict/25682
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv IOC/Fiocruz
publisher.none.fl_str_mv IOC/Fiocruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/25682/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/25682/2/A%20preliminary%20investigation...%20Regua%20Mangia.pdf
https://www.arca.fiocruz.br/bitstream/icict/25682/3/A%20preliminary%20investigation...%20Regua%20Mangia.pdf.txt
bitstream.checksum.fl_str_mv 8a4605be74aa9ea9d79846c1fba20a33
d9a62222248f647fc1faaa9ab1c3ca21
45acb56c3352209a0376d29bd6e5eeed
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1813009122335916032

Registros relacionados