Leishmanicidal activity of Cecropia pachystachya flavonoids: arginase inhibition and altered mitochondrial DNA arrangement

Detalhes bibliográficos
Autor(a) principal: Cruz, Ebenézer de Mello
Data de Publicação: 2013
Outros Autores: Silva, Edson Roberto da, Maquiaveli, Claudia do Carmo, Alves, Eliomara Sousa Sobral, Lucon Junior, João Francisco, Reis, Matheus Balduino Gonçalves dos, Toledo, Cleyton Eduardo Mendes de, Cruz, Frederico Guaré, Santos, Marcos André Vannier dos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7191
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Biologia Parasitária. Salvador, BA, Brasil
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spelling Cruz, Ebenézer de MelloSilva, Edson Roberto daMaquiaveli, Claudia do CarmoAlves, Eliomara Sousa SobralLucon Junior, João FranciscoReis, Matheus Balduino Gonçalves dosToledo, Cleyton Eduardo Mendes deCruz, Frederico GuaréSantos, Marcos André Vannier dos2013-10-23T17:55:30Z2013-10-23T17:55:30Z2013CRUZ, E. de M. et al. Leishmanicidal activity of Cecropia pachystachya flavonoids: arginase inhibition and altered mitochondrial DNA arrangement. Phytochemistry, v. 89, p. 71-77, 2013.1873-3700https://www.arca.fiocruz.br/handle/icict/7191engElsevierLeishmanicidal activity of Cecropia pachystachya flavonoids: arginase inhibition and altered mitochondrial DNA arrangementinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Biologia Parasitária. Salvador, BA, BrasilUniversidade de São Paulo. Faculdade de Zootecnia e Engenharia de Alimentos. Departamento de Medicina Veterinária. São Paulo, SP, BRasilUniversidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Fisiologia. Ribeirão Preto, SP, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Biologia Parasitária. Salvador, BA, BrasilUniversidade de São Paulo. Faculdade de Zootecnia e Engenharia de Alimentos. Pirassununga, SP, BrazilUniversidade de São Paulo. Faculdade de Zootecnia e Engenharia de Alimentos. Departamento de Medicina Veterinária. São Paulo, SP, BRasilCurso de Farmácia. Unidade de Ensino Superior Ingá. Maringa, SP, BrazilUniversidade Federal da Bahia. Instituto de Química. Departamento de Química Orgânica. Salvador, BA, BrazilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Biologia Parasitária. Salvador, BA, BrasilThe plant Cecropia pachystachya Trécul is widely used in Brazilian ethnomedicine to treat hypertension, asthma, and diabetes. Arginase is an enzyme with levels that are elevated in these disorders, and it is central to Leishmania polyamine biosynthesis. The aims of this study were to evaluate antileishmanial activity and inhibition of the arginase enzyme by C. pachystachya extracts, and to study changes in cellular organization using electron microscopy. The ethanol extract of C. pachystachya was tested on Leishmania (Leishmania) amazonensis promastigote survival/proliferation and arginase activity in vitro. Qualitative ultrastructural analysis was also used to observe changes in cell organization. The major bioactive molecules of the ethanol extract were characterized using liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS). The ethyl acetate fraction of the ethanol extract diminished promastigote axenic growth/survival, inhibited arginase activity, and altered a mitochondrial kinetoplast DNA (K-DNA) array. The bioactive compounds of C. pachystachya were characterized as glucoside flavonoids. Orientin (9) (luteolin-8-C-glucoside) was the main component of the methanol-soluble ethyl acetate fraction obtained from the ethanol extract and is an arginase inhibitor (IC50 15.9 µM). The ethyl acetate fraction was not cytotoxic to splenocytes at a concentration of 200 µg/mL. In conclusion, C. pachystachya contains bioactive compounds that reduce the growth of L. (L.) amazonensis promastigotes, altering mitochondrial K-DNA arrangement and inhibiting arginase.Cecropia pachystachyaCecropiaceaeArginasePolyaminesOxidative stressKinetoplastUltrastructureLeishmaniaAntiprotozoários/farmacologiaArginase/antagonistas & inibidoresCecropia (Planta)/químicaDNA Mitocondrial/genéticaInibidores Enzimáticos/farmacologiaFlavonoides/farmacologiaLeishmania/efeitos de drogasAcetatos/químicaEtanol/químicaGlucosídeos/farmacologiaHumanosLeishmaniaMetanol/químicaSolubilidadeinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALCruz, ED Leishmanicidal activity....pdfCruz, ED Leishmanicidal activity....pdfapplication/pdf753862https://www.arca.fiocruz.br/bitstream/icict/7191/1/Cruz%2c%20ED%20Leishmanicidal%20activity....pdfc99ee95789e442d656e72a0f4b9edb0cMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7191/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTCruz, ED Leishmanicidal activity....pdf.txtCruz, ED Leishmanicidal activity....pdf.txtExtracted texttext/plain42560https://www.arca.fiocruz.br/bitstream/icict/7191/5/Cruz%2c%20ED%20Leishmanicidal%20activity....pdf.txte755003bb5719356d3c50dbeb1cc2eeaMD55THUMBNAILCruz, ED Leishmanicidal activity....pdf.jpgCruz, ED Leishmanicidal activity....pdf.jpgGenerated Thumbnailimage/jpeg2526https://www.arca.fiocruz.br/bitstream/icict/7191/4/Cruz%2c%20ED%20Leishmanicidal%20activity....pdf.jpg1e93bd42fa9e6e0ffb6e8467c261ab84MD54icict/71912023-03-15 14:33:25.955oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:25Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Leishmanicidal activity of Cecropia pachystachya flavonoids: arginase inhibition and altered mitochondrial DNA arrangement
title Leishmanicidal activity of Cecropia pachystachya flavonoids: arginase inhibition and altered mitochondrial DNA arrangement
spellingShingle Leishmanicidal activity of Cecropia pachystachya flavonoids: arginase inhibition and altered mitochondrial DNA arrangement
Cruz, Ebenézer de Mello
Cecropia pachystachya
Cecropiaceae
Arginase
Polyamines
Oxidative stress
Kinetoplast
Ultrastructure
Leishmania
Antiprotozoários/farmacologia
Arginase/antagonistas & inibidores
Cecropia (Planta)/química
DNA Mitocondrial/genética
Inibidores Enzimáticos/farmacologia
Flavonoides/farmacologia
Leishmania/efeitos de drogas
Acetatos/química
Etanol/química
Glucosídeos/farmacologia
Humanos
Leishmania
Metanol/química
Solubilidade
title_short Leishmanicidal activity of Cecropia pachystachya flavonoids: arginase inhibition and altered mitochondrial DNA arrangement
title_full Leishmanicidal activity of Cecropia pachystachya flavonoids: arginase inhibition and altered mitochondrial DNA arrangement
title_fullStr Leishmanicidal activity of Cecropia pachystachya flavonoids: arginase inhibition and altered mitochondrial DNA arrangement
title_full_unstemmed Leishmanicidal activity of Cecropia pachystachya flavonoids: arginase inhibition and altered mitochondrial DNA arrangement
title_sort Leishmanicidal activity of Cecropia pachystachya flavonoids: arginase inhibition and altered mitochondrial DNA arrangement
author Cruz, Ebenézer de Mello
author_facet Cruz, Ebenézer de Mello
Silva, Edson Roberto da
Maquiaveli, Claudia do Carmo
Alves, Eliomara Sousa Sobral
Lucon Junior, João Francisco
Reis, Matheus Balduino Gonçalves dos
Toledo, Cleyton Eduardo Mendes de
Cruz, Frederico Guaré
Santos, Marcos André Vannier dos
author_role author
author2 Silva, Edson Roberto da
Maquiaveli, Claudia do Carmo
Alves, Eliomara Sousa Sobral
Lucon Junior, João Francisco
Reis, Matheus Balduino Gonçalves dos
Toledo, Cleyton Eduardo Mendes de
Cruz, Frederico Guaré
Santos, Marcos André Vannier dos
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Cruz, Ebenézer de Mello
Silva, Edson Roberto da
Maquiaveli, Claudia do Carmo
Alves, Eliomara Sousa Sobral
Lucon Junior, João Francisco
Reis, Matheus Balduino Gonçalves dos
Toledo, Cleyton Eduardo Mendes de
Cruz, Frederico Guaré
Santos, Marcos André Vannier dos
dc.subject.en.pt_BR.fl_str_mv Cecropia pachystachya
Cecropiaceae
Arginase
Polyamines
Oxidative stress
Kinetoplast
Ultrastructure
Leishmania
topic Cecropia pachystachya
Cecropiaceae
Arginase
Polyamines
Oxidative stress
Kinetoplast
Ultrastructure
Leishmania
Antiprotozoários/farmacologia
Arginase/antagonistas & inibidores
Cecropia (Planta)/química
DNA Mitocondrial/genética
Inibidores Enzimáticos/farmacologia
Flavonoides/farmacologia
Leishmania/efeitos de drogas
Acetatos/química
Etanol/química
Glucosídeos/farmacologia
Humanos
Leishmania
Metanol/química
Solubilidade
dc.subject.decs.pt_BR.fl_str_mv Antiprotozoários/farmacologia
Arginase/antagonistas & inibidores
Cecropia (Planta)/química
DNA Mitocondrial/genética
Inibidores Enzimáticos/farmacologia
Flavonoides/farmacologia
Leishmania/efeitos de drogas
Acetatos/química
Etanol/química
Glucosídeos/farmacologia
Humanos
Leishmania
Metanol/química
Solubilidade
description Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Biologia Parasitária. Salvador, BA, Brasil
publishDate 2013
dc.date.accessioned.fl_str_mv 2013-10-23T17:55:30Z
dc.date.available.fl_str_mv 2013-10-23T17:55:30Z
dc.date.issued.fl_str_mv 2013
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CRUZ, E. de M. et al. Leishmanicidal activity of Cecropia pachystachya flavonoids: arginase inhibition and altered mitochondrial DNA arrangement. Phytochemistry, v. 89, p. 71-77, 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7191
dc.identifier.issn.none.fl_str_mv 1873-3700
identifier_str_mv CRUZ, E. de M. et al. Leishmanicidal activity of Cecropia pachystachya flavonoids: arginase inhibition and altered mitochondrial DNA arrangement. Phytochemistry, v. 89, p. 71-77, 2013.
1873-3700
url https://www.arca.fiocruz.br/handle/icict/7191
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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