Leishmanicidal activity of Cecropia pachystachya flavonoids: Arginase inhibition and altered mitochondrial DNA arrangement
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2013 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFBA |
| Texto Completo: | http://www.repositorio.ufba.br/ri/handle/ri/11923 |
Resumo: | Texto completo. Acesso restrito. p. 71–77 |
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Cruz, Ebenézer de MelloSilva, Edson Roberto daMaquiaveli, Claudia do CarmoAlves, Eliomara Sousa SobralLucon Junior, João FranciscoReis, Matheus Balduino Gonçalves dosToledo, Cleyton Eduardo Mendes deCruz, Frederico GuareSantos, Marcos André Vannier dosCruz, Ebenézer de MelloSilva, Edson Roberto daMaquiaveli, Claudia do CarmoAlves, Eliomara Sousa SobralLucon Junior, João FranciscoReis, Matheus Balduino Gonçalves dosToledo, Cleyton Eduardo Mendes deCruz, Frederico GuareSantos, Marcos André Vannier dos2013-06-13T19:33:48Z2013-06-13T19:33:48Z20130031-9422http://www.repositorio.ufba.br/ri/handle/ri/11923v. 89Texto completo. Acesso restrito. p. 71–77The plant Cecropia pachystachya Trécul is widely used in Brazilian ethnomedicine to treat hypertension, asthma, and diabetes. Arginase is an enzyme with levels that are elevated in these disorders, and it is central to Leishmania polyamine biosynthesis. The aims of this study were to evaluate antileishmanial activity and inhibition of the arginase enzyme by C. pachystachya extracts, and to study changes in cellular organization using electron microscopy. The ethanol extract of C. pachystachya was tested on Leishmania (Leishmania) amazonensis promastigote survival/proliferation and arginase activity in vitro. Qualitative ultrastructural analysis was also used to observe changes in cell organization. The major bioactive molecules of the ethanol extract were characterized using liquid chromatography–electrospray ionizationmass spectrometry (LC–ESI-MS). The ethyl acetate fraction of the ethanol extract diminished promastigote axenic growth/survival, inhibited arginase activity, and altered a mitochondrial kinetoplast DNA (K-DNA) array. The bioactive compounds of C. pachystachya were characterized as glucoside flavonoids. Orientin (9) (luteolin-8-C-glucoside) was the main component of the methanol-soluble ethyl acetate fraction obtained from the ethanol extract and is an arginase inhibitor (IC50 15.9 lM). The ethyl acetate fraction was not cytotoxic to splenocytes at a concentration of 200 lg/mL. In conclusion, C. pachystachya contains bioactive compounds that reduce the growth of L. (L.) amazonensis promastigotes, altering mitochondrial K-DNA arrangement and inhibiting arginase.Submitted by Santiago Fabio (fabio.ssantiago@hotmail.com) on 2013-06-13T19:33:48Z No. of bitstreams: 1 1111111kk.pdf: 753862 bytes, checksum: c99ee95789e442d656e72a0f4b9edb0c (MD5)Made available in DSpace on 2013-06-13T19:33:48Z (GMT). No. of bitstreams: 1 1111111kk.pdf: 753862 bytes, checksum: c99ee95789e442d656e72a0f4b9edb0c (MD5) Previous issue date: 2013SalvadorPhytochemistryhttp://dx.doi.org/10.1016/j.phytochem.2013.01.014reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBACecropia pachystachyaCecropiaceaeArginasePolyaminesOxidative stressKinetoplastUltrastructureLeishmaniaLeishmanicidal activity of Cecropia pachystachya flavonoids: Arginase inhibition and altered mitochondrial DNA arrangementPhytochemistryinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleenginfo:eu-repo/semantics/openAccessORIGINAL1111111kk.pdf1111111kk.pdfapplication/pdf753862https://repositorio.ufba.br/bitstream/ri/11923/1/1111111kk.pdfc99ee95789e442d656e72a0f4b9edb0cMD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/11923/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXT1111111kk.pdf.txt1111111kk.pdf.txtExtracted texttext/plain42535https://repositorio.ufba.br/bitstream/ri/11923/3/1111111kk.pdf.txt55a7094f7336da81be915c2052303fa2MD53ri/119232022-08-08 13:17:49.297oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-08-08T16:17:49Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
| dc.title.pt_BR.fl_str_mv |
Leishmanicidal activity of Cecropia pachystachya flavonoids: Arginase inhibition and altered mitochondrial DNA arrangement |
| dc.title.alternative.pt_BR.fl_str_mv |
Phytochemistry |
| title |
Leishmanicidal activity of Cecropia pachystachya flavonoids: Arginase inhibition and altered mitochondrial DNA arrangement |
| spellingShingle |
Leishmanicidal activity of Cecropia pachystachya flavonoids: Arginase inhibition and altered mitochondrial DNA arrangement Cruz, Ebenézer de Mello Cecropia pachystachya Cecropiaceae Arginase Polyamines Oxidative stress Kinetoplast Ultrastructure Leishmania |
| title_short |
Leishmanicidal activity of Cecropia pachystachya flavonoids: Arginase inhibition and altered mitochondrial DNA arrangement |
| title_full |
Leishmanicidal activity of Cecropia pachystachya flavonoids: Arginase inhibition and altered mitochondrial DNA arrangement |
| title_fullStr |
Leishmanicidal activity of Cecropia pachystachya flavonoids: Arginase inhibition and altered mitochondrial DNA arrangement |
| title_full_unstemmed |
Leishmanicidal activity of Cecropia pachystachya flavonoids: Arginase inhibition and altered mitochondrial DNA arrangement |
| title_sort |
Leishmanicidal activity of Cecropia pachystachya flavonoids: Arginase inhibition and altered mitochondrial DNA arrangement |
| author |
Cruz, Ebenézer de Mello |
| author_facet |
Cruz, Ebenézer de Mello Silva, Edson Roberto da Maquiaveli, Claudia do Carmo Alves, Eliomara Sousa Sobral Lucon Junior, João Francisco Reis, Matheus Balduino Gonçalves dos Toledo, Cleyton Eduardo Mendes de Cruz, Frederico Guare Santos, Marcos André Vannier dos |
| author_role |
author |
| author2 |
Silva, Edson Roberto da Maquiaveli, Claudia do Carmo Alves, Eliomara Sousa Sobral Lucon Junior, João Francisco Reis, Matheus Balduino Gonçalves dos Toledo, Cleyton Eduardo Mendes de Cruz, Frederico Guare Santos, Marcos André Vannier dos |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Cruz, Ebenézer de Mello Silva, Edson Roberto da Maquiaveli, Claudia do Carmo Alves, Eliomara Sousa Sobral Lucon Junior, João Francisco Reis, Matheus Balduino Gonçalves dos Toledo, Cleyton Eduardo Mendes de Cruz, Frederico Guare Santos, Marcos André Vannier dos Cruz, Ebenézer de Mello Silva, Edson Roberto da Maquiaveli, Claudia do Carmo Alves, Eliomara Sousa Sobral Lucon Junior, João Francisco Reis, Matheus Balduino Gonçalves dos Toledo, Cleyton Eduardo Mendes de Cruz, Frederico Guare Santos, Marcos André Vannier dos |
| dc.subject.por.fl_str_mv |
Cecropia pachystachya Cecropiaceae Arginase Polyamines Oxidative stress Kinetoplast Ultrastructure Leishmania |
| topic |
Cecropia pachystachya Cecropiaceae Arginase Polyamines Oxidative stress Kinetoplast Ultrastructure Leishmania |
| description |
Texto completo. Acesso restrito. p. 71–77 |
| publishDate |
2013 |
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2013-06-13T19:33:48Z |
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2013-06-13T19:33:48Z |
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2013 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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http://www.repositorio.ufba.br/ri/handle/ri/11923 |
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0031-9422 |
| dc.identifier.number.pt_BR.fl_str_mv |
v. 89 |
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0031-9422 v. 89 |
| url |
http://www.repositorio.ufba.br/ri/handle/ri/11923 |
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eng |
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openAccess |
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Phytochemistry |
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Phytochemistry |
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http://dx.doi.org/10.1016/j.phytochem.2013.01.014 |
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reponame:Repositório Institucional da UFBA instname:Universidade Federal da Bahia (UFBA) instacron:UFBA |
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