Evaluation of Genetic Polymorphism of Leishmania (V.) braziliensis Isolates Obtained from the Same Patient before and after Therapeutic Failure or Reactivation of Cutaneous Lesions
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2012 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/18360 |
Resumo: | Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Vigilância em Leishmanioses. Rio de Janeiro, RJ, Brasil. |
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Baptista, CibeleSchubach, Armando de OliveiraMadeira, Maria de FátimaMiranda, Luciana de Freitas CamposPinto, Andressa Guimarães de SouzaBarros, Juliana Helena da SilvaSilva, Fátima ConceiçãoPimentel, Maria Ines FernandesPacheco, Raquel da Silva2017-04-11T12:04:41Z2017-04-11T12:04:41Z2012BAPTISTA, Cibele et al. Evaluation of Genetic Polymorphism of Leishmania (V.) braziliensis Isolates Obtained fromthe Same Patient before and after Therapeutic Failure or Reactivation of Cutaneous Lesions. Journal of Tropical Medicine, v. 2012, p. 1-5, 2012.1687-9686https://www.arca.fiocruz.br/handle/icict/1836010.1155/2012/8081321687-9694engHindawi Publishing CorporationLeishmania brasiliensisPolimorfismo GenéticoFalha terapêuticaLesões cutâneasLeishmania brasiliensisGenetic polymorphismTherapeutic FailureCutaneous LesionsEvaluation of Genetic Polymorphism of Leishmania (V.) braziliensis Isolates Obtained from the Same Patient before and after Therapeutic Failure or Reactivation of Cutaneous Lesionsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Vigilância em Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Vigilância em Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Vigilância em Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Vigilância em Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Vigilância em Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Vigilância em Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Vigilância em Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Sistemática Bioquímica. Rio de Janeiro, RJ, Brasil.The aim of this study was to investigate genetic polymorphism in Leishmania braziliensis population previously typed through isoenzyme electrophoresis, isolated from the same patient in two different moments: (A) before the beginning of treatment and (B) after treatment failure to meglumine antimoniate or reactivation after successful initial treatment. Fifteen pairs of isolates were assessed using the polymorphic molecular marker LSSP-PCR and following the phenetic analysis. The genetic profiles of the 30 samples were grouped in four clusters. Only two patients presented total identity in the A and B isolates. Most isolates presented similarity coefficients varying from 0.63 to 0.91. In this group of patients genetic polymorphisms could be observed indicating low similarity between the pairs of isolates. The results demonstrate the existence of genetic polymorphism between the samples isolated before treatment and after reactivation or treatment failure, suggesting a possible differentiation of the structure of the original parasite population which could be involved in the mechanisms of resistance to treatment or reactivation of lesions in the ATL. This phenomenon is important, although other factors also could be involved in this context and are discussed in this paper.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/18360/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALfatima_silva_etal_IOC_2012.pdffatima_silva_etal_IOC_2012.pdfapplication/pdf1229743https://www.arca.fiocruz.br/bitstream/icict/18360/2/fatima_silva_etal_IOC_2012.pdf564bb69ce587819ac59bd71df9b3059dMD52TEXTfatima_silva_etal_IOC_2012.pdf.txtfatima_silva_etal_IOC_2012.pdf.txtExtracted texttext/plain23165https://www.arca.fiocruz.br/bitstream/icict/18360/3/fatima_silva_etal_IOC_2012.pdf.txt87cfd74d341c78cf78ccefe06a681461MD53icict/183602023-09-14 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| dc.title.pt_BR.fl_str_mv |
Evaluation of Genetic Polymorphism of Leishmania (V.) braziliensis Isolates Obtained from the Same Patient before and after Therapeutic Failure or Reactivation of Cutaneous Lesions |
| title |
Evaluation of Genetic Polymorphism of Leishmania (V.) braziliensis Isolates Obtained from the Same Patient before and after Therapeutic Failure or Reactivation of Cutaneous Lesions |
| spellingShingle |
Evaluation of Genetic Polymorphism of Leishmania (V.) braziliensis Isolates Obtained from the Same Patient before and after Therapeutic Failure or Reactivation of Cutaneous Lesions Baptista, Cibele Leishmania brasiliensis Polimorfismo Genético Falha terapêutica Lesões cutâneas Leishmania brasiliensis Genetic polymorphism Therapeutic Failure Cutaneous Lesions |
| title_short |
Evaluation of Genetic Polymorphism of Leishmania (V.) braziliensis Isolates Obtained from the Same Patient before and after Therapeutic Failure or Reactivation of Cutaneous Lesions |
| title_full |
Evaluation of Genetic Polymorphism of Leishmania (V.) braziliensis Isolates Obtained from the Same Patient before and after Therapeutic Failure or Reactivation of Cutaneous Lesions |
| title_fullStr |
Evaluation of Genetic Polymorphism of Leishmania (V.) braziliensis Isolates Obtained from the Same Patient before and after Therapeutic Failure or Reactivation of Cutaneous Lesions |
| title_full_unstemmed |
Evaluation of Genetic Polymorphism of Leishmania (V.) braziliensis Isolates Obtained from the Same Patient before and after Therapeutic Failure or Reactivation of Cutaneous Lesions |
| title_sort |
Evaluation of Genetic Polymorphism of Leishmania (V.) braziliensis Isolates Obtained from the Same Patient before and after Therapeutic Failure or Reactivation of Cutaneous Lesions |
| author |
Baptista, Cibele |
| author_facet |
Baptista, Cibele Schubach, Armando de Oliveira Madeira, Maria de Fátima Miranda, Luciana de Freitas Campos Pinto, Andressa Guimarães de Souza Barros, Juliana Helena da Silva Silva, Fátima Conceição Pimentel, Maria Ines Fernandes Pacheco, Raquel da Silva |
| author_role |
author |
| author2 |
Schubach, Armando de Oliveira Madeira, Maria de Fátima Miranda, Luciana de Freitas Campos Pinto, Andressa Guimarães de Souza Barros, Juliana Helena da Silva Silva, Fátima Conceição Pimentel, Maria Ines Fernandes Pacheco, Raquel da Silva |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Baptista, Cibele Schubach, Armando de Oliveira Madeira, Maria de Fátima Miranda, Luciana de Freitas Campos Pinto, Andressa Guimarães de Souza Barros, Juliana Helena da Silva Silva, Fátima Conceição Pimentel, Maria Ines Fernandes Pacheco, Raquel da Silva |
| dc.subject.other.pt_BR.fl_str_mv |
Leishmania brasiliensis Polimorfismo Genético Falha terapêutica Lesões cutâneas |
| topic |
Leishmania brasiliensis Polimorfismo Genético Falha terapêutica Lesões cutâneas Leishmania brasiliensis Genetic polymorphism Therapeutic Failure Cutaneous Lesions |
| dc.subject.en.pt_BR.fl_str_mv |
Leishmania brasiliensis Genetic polymorphism Therapeutic Failure Cutaneous Lesions |
| description |
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Vigilância em Leishmanioses. Rio de Janeiro, RJ, Brasil. |
| publishDate |
2012 |
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2012 |
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2017-04-11T12:04:41Z |
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2017-04-11T12:04:41Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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BAPTISTA, Cibele et al. Evaluation of Genetic Polymorphism of Leishmania (V.) braziliensis Isolates Obtained fromthe Same Patient before and after Therapeutic Failure or Reactivation of Cutaneous Lesions. Journal of Tropical Medicine, v. 2012, p. 1-5, 2012. |
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https://www.arca.fiocruz.br/handle/icict/18360 |
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1687-9686 |
| dc.identifier.doi.none.fl_str_mv |
10.1155/2012/808132 |
| dc.identifier.eissn.none.fl_str_mv |
1687-9694 |
| identifier_str_mv |
BAPTISTA, Cibele et al. Evaluation of Genetic Polymorphism of Leishmania (V.) braziliensis Isolates Obtained fromthe Same Patient before and after Therapeutic Failure or Reactivation of Cutaneous Lesions. Journal of Tropical Medicine, v. 2012, p. 1-5, 2012. 1687-9686 10.1155/2012/808132 1687-9694 |
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https://www.arca.fiocruz.br/handle/icict/18360 |
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Hindawi Publishing Corporation |
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Hindawi Publishing Corporation |
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