Synthetic Peptides Derived from Ribosomal Proteins of Leishmania spp. in Mucocutaneous Leishmaniasis: Diagnostic Usefulness

Detalhes bibliográficos
Autor(a) principal: Florez, Magda Melissa
Data de Publicação: 2017
Outros Autores: Oliveira, Camila Indiani de, Puerta, Concepcion, Guzman, Fanny, Ayala, Martha, Montoya, Gladis, Delgado, Gabriela
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/25014
Resumo: Universidad Nacional de Colombia’s Bogotá Research Division (DIB) project 16015, as well as a grant from the Colombian Science, Technology and Innovation Department’s (Colciencias) Young Researchers and Innovators programme "Virginia Gutiérrez de Pineda" (2012) and Colciencias Grant (contract number 03092013, code 57635945- project 6).
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spelling Florez, Magda MelissaOliveira, Camila Indiani dePuerta, ConcepcionGuzman, FannyAyala, MarthaMontoya, GladisDelgado, Gabriela2018-03-01T13:19:56Z2018-03-01T13:19:56Z2017FLOREZ, M. M. et al. Synthetic Peptides Derived from Ribosomal Proteins of Leishmania spp. in Mucocutaneous Leishmaniasis: Diagnostic Usefulness. Protein and Peptide Letters, v. 24, p. 982-988, 2017.0929-8665https://www.arca.fiocruz.br/handle/icict/2501410.2174/0929866524666170728143924Universidad Nacional de Colombia’s Bogotá Research Division (DIB) project 16015, as well as a grant from the Colombian Science, Technology and Innovation Department’s (Colciencias) Young Researchers and Innovators programme "Virginia Gutiérrez de Pineda" (2012) and Colciencias Grant (contract number 03092013, code 57635945- project 6).Universidad Nacional de Colombia. Facultad de Ciencias Grupo de Investigación en Inmunotoxicología. Departamento de Farmacia. Bogotá, ColombiaFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Imunoparasitologia. Salvador, BA, BrasilPontificia Universidad Javeriana. Facultad de Ciencias. Grupo de Investigación en Enfermedades Infecciosas. Bogotá, ColombiaUniversidad Católica de Valparaíso. Pontificia Curauma. Núcleo de Biolotecnología Curauma. Valparaiso, ChileInstituto Nacional de Salud. Laboratorio de Parasitología. Bogotá, ColombiaUniversidad del Rosario. Centro de Estudio de Enfermedades Autoinmunes. Bogotá, ColombiaUniversidad Nacional de Colombia. Facultad de Ciencias Grupo de Investigación en Inmunotoxicología. Departamento de Farmacia. Bogotá, ColombiaThe serological diagnostic methods currently available for mucocutaneous leishmaniasis (MCL) lack specificity when complete parasites are used; however, such specificity increases when protein fractions are used. Ribosomal proteins have been reported to induce antibodies in animal and humans infected with the parasite, making them a worth candidate to assess its diagnosis potential. Objective: This study was thus aimed at evaluating synthetic peptides derived from Leishmania braziliensis ribosomal proteins S25 and S5 as antigen candidates for diagnosing MCL by ELISA Methods: It was used 8 and 13 peptides derived from ribosomal proteins 25 and S5 respectively as antigens in order to detect IgG antibodies by ELISA in people with active MCL, Chagas disease (CH) and autoimmune disease (AID). Results: 4 of these 21 peptides (P4, P6, P19 and P21) had the greatest sensitivity (21.7%, 13.04%, 20% and 20%, respectively) as well as having 95%, 100%, 100% and 82.5% specificity, respectively. Conclusion: The study revealed the limited usefulness of the peptides being studied as a diagnostic tool in the conditions used here, because its low sensitivity, but it is worth highlighting that the use of peptides as antigen in the serodiagnosis of MCL may overcome the cross reaction presented with other antigens, thus avoiding false positives.engBentham Science PublishersLeishmaniose mucocutâneaDiagnósticoPéptido sintéticoProteína ribossômicaParasitaLeishmania braziliensisMucocutaneous leishmaniasisDiagnosisSynthetic peptideRibosomal proteinParasiteLeishmania braziliensisSynthetic Peptides Derived from Ribosomal Proteins of Leishmania spp. in Mucocutaneous Leishmaniasis: Diagnostic Usefulnessinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv Synthetic Peptides Derived from Ribosomal Proteins of Leishmania spp. in Mucocutaneous Leishmaniasis: Diagnostic Usefulness
title Synthetic Peptides Derived from Ribosomal Proteins of Leishmania spp. in Mucocutaneous Leishmaniasis: Diagnostic Usefulness
spellingShingle Synthetic Peptides Derived from Ribosomal Proteins of Leishmania spp. in Mucocutaneous Leishmaniasis: Diagnostic Usefulness
Florez, Magda Melissa
Leishmaniose mucocutânea
Diagnóstico
Péptido sintético
Proteína ribossômica
Parasita
Leishmania braziliensis
Mucocutaneous leishmaniasis
Diagnosis
Synthetic peptide
Ribosomal protein
Parasite
Leishmania braziliensis
title_short Synthetic Peptides Derived from Ribosomal Proteins of Leishmania spp. in Mucocutaneous Leishmaniasis: Diagnostic Usefulness
title_full Synthetic Peptides Derived from Ribosomal Proteins of Leishmania spp. in Mucocutaneous Leishmaniasis: Diagnostic Usefulness
title_fullStr Synthetic Peptides Derived from Ribosomal Proteins of Leishmania spp. in Mucocutaneous Leishmaniasis: Diagnostic Usefulness
title_full_unstemmed Synthetic Peptides Derived from Ribosomal Proteins of Leishmania spp. in Mucocutaneous Leishmaniasis: Diagnostic Usefulness
title_sort Synthetic Peptides Derived from Ribosomal Proteins of Leishmania spp. in Mucocutaneous Leishmaniasis: Diagnostic Usefulness
author Florez, Magda Melissa
author_facet Florez, Magda Melissa
Oliveira, Camila Indiani de
Puerta, Concepcion
Guzman, Fanny
Ayala, Martha
Montoya, Gladis
Delgado, Gabriela
author_role author
author2 Oliveira, Camila Indiani de
Puerta, Concepcion
Guzman, Fanny
Ayala, Martha
Montoya, Gladis
Delgado, Gabriela
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Florez, Magda Melissa
Oliveira, Camila Indiani de
Puerta, Concepcion
Guzman, Fanny
Ayala, Martha
Montoya, Gladis
Delgado, Gabriela
dc.subject.other.pt_BR.fl_str_mv Leishmaniose mucocutânea
Diagnóstico
Péptido sintético
Proteína ribossômica
Parasita
Leishmania braziliensis
topic Leishmaniose mucocutânea
Diagnóstico
Péptido sintético
Proteína ribossômica
Parasita
Leishmania braziliensis
Mucocutaneous leishmaniasis
Diagnosis
Synthetic peptide
Ribosomal protein
Parasite
Leishmania braziliensis
dc.subject.en.pt_BR.fl_str_mv Mucocutaneous leishmaniasis
Diagnosis
Synthetic peptide
Ribosomal protein
Parasite
Leishmania braziliensis
description Universidad Nacional de Colombia’s Bogotá Research Division (DIB) project 16015, as well as a grant from the Colombian Science, Technology and Innovation Department’s (Colciencias) Young Researchers and Innovators programme "Virginia Gutiérrez de Pineda" (2012) and Colciencias Grant (contract number 03092013, code 57635945- project 6).
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2018-03-01T13:19:56Z
dc.date.available.fl_str_mv 2018-03-01T13:19:56Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FLOREZ, M. M. et al. Synthetic Peptides Derived from Ribosomal Proteins of Leishmania spp. in Mucocutaneous Leishmaniasis: Diagnostic Usefulness. Protein and Peptide Letters, v. 24, p. 982-988, 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/25014
dc.identifier.issn.pt_BR.fl_str_mv 0929-8665
dc.identifier.doi.none.fl_str_mv 10.2174/0929866524666170728143924
identifier_str_mv FLOREZ, M. M. et al. Synthetic Peptides Derived from Ribosomal Proteins of Leishmania spp. in Mucocutaneous Leishmaniasis: Diagnostic Usefulness. Protein and Peptide Letters, v. 24, p. 982-988, 2017.
0929-8665
10.2174/0929866524666170728143924
url https://www.arca.fiocruz.br/handle/icict/25014
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Bentham Science Publishers
publisher.none.fl_str_mv Bentham Science Publishers
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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