A Simple Approach to Analyze Sugar Nectar Composition in Flowers Using Capillary Electrophoresis and Enzymatic Assays

Detalhes bibliográficos
Autor(a) principal: Bordin, Dayanne M.
Data de Publicação: 2020
Outros Autores: Latgé, Samara G. C., Pyke, Graham, Kalman, John, Doble, Philip, Genta, Fernando A., Blanes, Lucas
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/43169
Resumo: University of Technology Sydney. School of Mathematical and Physical Sciences. New South Wales, Australia.
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spelling Bordin, Dayanne M.Latgé, Samara G. C.Pyke, GrahamKalman, JohnDoble, PhilipGenta, Fernando A.Blanes, Lucas2020-09-03T17:49:22Z2020-09-03T17:49:22Z2020BORDIN, Dayanne M. et al. A Simple Approach to Analyze Sugar Nectar Composition in Flowers Using Capillary Electrophoresis and Enzymatic Assays. Journal of the Brazilian Chemical Society, v. 31, n. 10, p. 2129-2134, 2020.0103-5053https://www.arca.fiocruz.br/handle/icict/4316910.21577/0103-5053.202001141678-4790engUniversidade de CampinasEletroforese capilarNéctarSacaroseGlicoseFrutoseCapillary electrophoresisNectarSucroseGlucoseFructoseA Simple Approach to Analyze Sugar Nectar Composition in Flowers Using Capillary Electrophoresis and Enzymatic Assaysinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversity of Technology Sydney. School of Mathematical and Physical Sciences. New South Wales, Australia.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil.Macquarie University. Department of Biological Sciences. New South Wales, Australia.University of Technology Sydney. School of Mathematical and Physical Sciences. New South Wales, Australia.University of Technology Sydney. School of Mathematical and Physical Sciences. New South Wales, Australia.Macquarie University. Department of Biological Sciences. New South Wales, Australia.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil.Despite the obvious importance of nectar, the composition of this solution remains surprisingly understudied to most of the flowers. Here we describe a simple, low-cost, and reliable methodology to analyze the three main sugars present in 210 nectar samples of Christmas Bellflowers using capillary electrophoresis (CE) and enzymatic assays. CE separation (-16 kV) was made using an electrolyte containing 36 mM NaOH pH 12, 15 mM sorbic acid, 0.5 mM hexadecyl trimethylammonium bromide (CTAB), and indirect UV detection. 72.9% of analyzed nectar samples were sucrose dominant; 13.3% sucrose rich only and 13.8% were fructose rich. The concentrations of fructose and glucose present in most of the samples were very similar, while the amount of sucrose varied considerably. The average amounts of fructose, glucose, sucrose and total sugar were 0.22, 0.21, 0.34 and 0.8 M, respectively. The nectar sample shows a pH average of 5. α-Glucosidase activity was observed in 65% of the samples with an average activity of 0.013 µU µL-1 providing a possible explanation for the similar concentrations of fructose and glucose in the samples. Under our knowledge, this is the first article that shows the analysis of sugars in nectar flowers using CE.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/43169/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSamaraGC_Latge_etal_IOC_2020.pdfSamaraGC_Latge_etal_IOC_2020.pdfapplication/pdf217114https://www.arca.fiocruz.br/bitstream/icict/43169/2/SamaraGC_Latge_etal_IOC_2020.pdfe4a8bdb1f30b19f2c31c1e3e01596227MD52TEXTSamaraGC_Latge_etal_IOC_2020.pdf.txtSamaraGC_Latge_etal_IOC_2020.pdf.txtExtracted texttext/plain25466https://www.arca.fiocruz.br/bitstream/icict/43169/3/SamaraGC_Latge_etal_IOC_2020.pdf.txt31715d93cccf8b7bf2e741f1018ffaebMD53icict/431692020-12-01 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dc.title.pt_BR.fl_str_mv A Simple Approach to Analyze Sugar Nectar Composition in Flowers Using Capillary Electrophoresis and Enzymatic Assays
title A Simple Approach to Analyze Sugar Nectar Composition in Flowers Using Capillary Electrophoresis and Enzymatic Assays
spellingShingle A Simple Approach to Analyze Sugar Nectar Composition in Flowers Using Capillary Electrophoresis and Enzymatic Assays
Bordin, Dayanne M.
Eletroforese capilar
Néctar
Sacarose
Glicose
Frutose
Capillary electrophoresis
Nectar
Sucrose
Glucose
Fructose
title_short A Simple Approach to Analyze Sugar Nectar Composition in Flowers Using Capillary Electrophoresis and Enzymatic Assays
title_full A Simple Approach to Analyze Sugar Nectar Composition in Flowers Using Capillary Electrophoresis and Enzymatic Assays
title_fullStr A Simple Approach to Analyze Sugar Nectar Composition in Flowers Using Capillary Electrophoresis and Enzymatic Assays
title_full_unstemmed A Simple Approach to Analyze Sugar Nectar Composition in Flowers Using Capillary Electrophoresis and Enzymatic Assays
title_sort A Simple Approach to Analyze Sugar Nectar Composition in Flowers Using Capillary Electrophoresis and Enzymatic Assays
author Bordin, Dayanne M.
author_facet Bordin, Dayanne M.
Latgé, Samara G. C.
Pyke, Graham
Kalman, John
Doble, Philip
Genta, Fernando A.
Blanes, Lucas
author_role author
author2 Latgé, Samara G. C.
Pyke, Graham
Kalman, John
Doble, Philip
Genta, Fernando A.
Blanes, Lucas
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Bordin, Dayanne M.
Latgé, Samara G. C.
Pyke, Graham
Kalman, John
Doble, Philip
Genta, Fernando A.
Blanes, Lucas
dc.subject.other.pt_BR.fl_str_mv Eletroforese capilar
Néctar
Sacarose
Glicose
Frutose
topic Eletroforese capilar
Néctar
Sacarose
Glicose
Frutose
Capillary electrophoresis
Nectar
Sucrose
Glucose
Fructose
dc.subject.en.pt_BR.fl_str_mv Capillary electrophoresis
Nectar
Sucrose
Glucose
Fructose
description University of Technology Sydney. School of Mathematical and Physical Sciences. New South Wales, Australia.
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-09-03T17:49:22Z
dc.date.available.fl_str_mv 2020-09-03T17:49:22Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BORDIN, Dayanne M. et al. A Simple Approach to Analyze Sugar Nectar Composition in Flowers Using Capillary Electrophoresis and Enzymatic Assays. Journal of the Brazilian Chemical Society, v. 31, n. 10, p. 2129-2134, 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/43169
dc.identifier.issn.pt_BR.fl_str_mv 0103-5053
dc.identifier.doi.none.fl_str_mv 10.21577/0103-5053.20200114
dc.identifier.eissn.none.fl_str_mv 1678-4790
identifier_str_mv BORDIN, Dayanne M. et al. A Simple Approach to Analyze Sugar Nectar Composition in Flowers Using Capillary Electrophoresis and Enzymatic Assays. Journal of the Brazilian Chemical Society, v. 31, n. 10, p. 2129-2134, 2020.
0103-5053
10.21577/0103-5053.20200114
1678-4790
url https://www.arca.fiocruz.br/handle/icict/43169
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade de Campinas
publisher.none.fl_str_mv Universidade de Campinas
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/43169/1/license.txt
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