Analysis of the genetic variability of PvMSP-3α among Plasmodium vivax in Brazilian field isolates

Detalhes bibliográficos
Autor(a) principal: Ribeiro, Ricardo Souza
Data de Publicação: 2011
Outros Autores: Ladeira, Luisa, Rezende, Antonio Mauro, Fontes, Cor Jesus Fernandes, Carvalho, Luzia Helena, Brito, Cristiana Ferreira Alves de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/9006
Resumo: FAPEMIG/PROBIC, Rede Malária/Pronex-CNPq, MS/DECIT, FAPEMIG, FAPEMAT, FAPERJ CFAB
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spelling Ribeiro, Ricardo SouzaLadeira, LuisaRezende, Antonio MauroFontes, Cor Jesus FernandesCarvalho, Luzia HelenaBrito, Cristiana Ferreira Alves de2014-11-28T17:06:50Z2014-11-28T17:06:50Z2011RIBEIRO, Ricardo Souza et al. Analysis of the genetic variability of PvMSP-3α among Plasmodium vivax in Brazilian field isolates. Memórias do Instituto Oswaldo Cruz, v. 106, Supl.1, p. 27-33, 2011.0074-0276https://www.arca.fiocruz.br/handle/icict/9006FAPEMIG/PROBIC, Rede Malária/Pronex-CNPq, MS/DECIT, FAPEMIG, FAPEMAT, FAPERJ CFABFundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Malária. Horizonte, MG, BrasilFundação Oswaldo. Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Malária. Horizonte, MG, BrasilFundação Oswaldo. Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Parasitologia Celular e Molecular. Horizonte, MG, BrasilUniversidade Federal do Mato Grosso. Departamento de Clínica Médica. Cuiabá, MT, BrasilFundação Oswaldo. Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Malária. Horizonte, MG, BrasilFundação Oswaldo. Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Malária. Horizonte, MG, BrasilReliable molecular markers are essential for a better understanding of the molecular epidemiology ofPlasmodium vivax, which is a neglected human malaria parasite. The aim of this study was to analyze the genetic diversity of P. vivax isolates from the Brazilian Amazon using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the highly polymorphic merozoite surface protein-3alpha(PvMSP-3α) gene. To accomplish this, 60 isolates of P. vivax from different endemic areas in the Brazilian Amazon were collected. The PvMSP-3α gene was amplified by nested-PCR. Three major types of the PvMSP-3αlocus were detected at different frequencies: type A (68%), B (15%) and C (17%). A single sample showed two PCR fragments, which corresponded to infection with types A and C. PCR-RFLP analysis using the HhaI restriction enzyme for 52 isolates clearly identified 11 haplotypes, eight of which were from type A, two from type B and only one from type C. Seven other isolates did not show a clear pattern using PCR-RFLP. This result might be due to multiple clone infections. This study showed a high diversity of the PvMSP-3α gene among P. vivaxisolates from the Brazilian Amazon, but also indicated that the detection performance of PCR-RFLP of the PvMSP-3α gene may not be sufficient to detect multiple clone infections.engFundação Oswaldo CruzAnalysis of the genetic variability of PvMSP-3α among Plasmodium vivax in Brazilian field isolatesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlemalariaPlasmodium vivaxgenetic variationantigenic variabilityMSP3 proteinrestriction fragment length polymorphisminfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/9006/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALAnalysis of the genetic variability of PvMSP-3α among Plasmodium vivax in Brazilian field isolates.pdfAnalysis of the genetic variability of PvMSP-3α among Plasmodium vivax in Brazilian field isolates.pdfapplication/pdf855235https://www.arca.fiocruz.br/bitstream/icict/9006/2/Analysis%20of%20the%20genetic%20variability%20of%20PvMSP-3%ce%b1%20among%20Plasmodium%20vivax%20in%20Brazilian%20field%20isolates.pdfb64d257bbe88f20511094531198deae5MD52TEXTAnalysis of the genetic variability of PvMSP-3α among Plasmodium vivax in Brazilian field isolates.pdf.txtAnalysis of the genetic variability of PvMSP-3α among Plasmodium vivax in Brazilian field isolates.pdf.txtExtracted texttext/plain36176https://www.arca.fiocruz.br/bitstream/icict/9006/3/Analysis%20of%20the%20genetic%20variability%20of%20PvMSP-3%ce%b1%20among%20Plasmodium%20vivax%20in%20Brazilian%20field%20isolates.pdf.txted0f24bc1eb3be39dc35a8dd628c17d2MD53icict/90062019-06-19 10:12:21.156oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-06-19T13:12:21Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Analysis of the genetic variability of PvMSP-3α among Plasmodium vivax in Brazilian field isolates
title Analysis of the genetic variability of PvMSP-3α among Plasmodium vivax in Brazilian field isolates
spellingShingle Analysis of the genetic variability of PvMSP-3α among Plasmodium vivax in Brazilian field isolates
Ribeiro, Ricardo Souza
malaria
Plasmodium vivax
genetic variation
antigenic variability
MSP3 protein
restriction fragment length polymorphism
title_short Analysis of the genetic variability of PvMSP-3α among Plasmodium vivax in Brazilian field isolates
title_full Analysis of the genetic variability of PvMSP-3α among Plasmodium vivax in Brazilian field isolates
title_fullStr Analysis of the genetic variability of PvMSP-3α among Plasmodium vivax in Brazilian field isolates
title_full_unstemmed Analysis of the genetic variability of PvMSP-3α among Plasmodium vivax in Brazilian field isolates
title_sort Analysis of the genetic variability of PvMSP-3α among Plasmodium vivax in Brazilian field isolates
author Ribeiro, Ricardo Souza
author_facet Ribeiro, Ricardo Souza
Ladeira, Luisa
Rezende, Antonio Mauro
Fontes, Cor Jesus Fernandes
Carvalho, Luzia Helena
Brito, Cristiana Ferreira Alves de
author_role author
author2 Ladeira, Luisa
Rezende, Antonio Mauro
Fontes, Cor Jesus Fernandes
Carvalho, Luzia Helena
Brito, Cristiana Ferreira Alves de
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Ribeiro, Ricardo Souza
Ladeira, Luisa
Rezende, Antonio Mauro
Fontes, Cor Jesus Fernandes
Carvalho, Luzia Helena
Brito, Cristiana Ferreira Alves de
dc.subject.en.pt_BR.fl_str_mv malaria
Plasmodium vivax
genetic variation
antigenic variability
MSP3 protein
restriction fragment length polymorphism
topic malaria
Plasmodium vivax
genetic variation
antigenic variability
MSP3 protein
restriction fragment length polymorphism
description FAPEMIG/PROBIC, Rede Malária/Pronex-CNPq, MS/DECIT, FAPEMIG, FAPEMAT, FAPERJ CFAB
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2014-11-28T17:06:50Z
dc.date.available.fl_str_mv 2014-11-28T17:06:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv RIBEIRO, Ricardo Souza et al. Analysis of the genetic variability of PvMSP-3α among Plasmodium vivax in Brazilian field isolates. Memórias do Instituto Oswaldo Cruz, v. 106, Supl.1, p. 27-33, 2011.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/9006
dc.identifier.issn.none.fl_str_mv 0074-0276
identifier_str_mv RIBEIRO, Ricardo Souza et al. Analysis of the genetic variability of PvMSP-3α among Plasmodium vivax in Brazilian field isolates. Memórias do Instituto Oswaldo Cruz, v. 106, Supl.1, p. 27-33, 2011.
0074-0276
url https://www.arca.fiocruz.br/handle/icict/9006
dc.language.iso.fl_str_mv eng
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