Erythema Nodosum Leprosum Neutrophil Subset Expressing IL-10R1 Transmigrates into Skin Lesions and Responds to IL-10
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/40455 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. |
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Pacheco, Fabiana SantosPrata, Rhana Berto da SilvaBrandão, Sheila SantosFerreira, HelenRodrigues, Thais FernandaSantos, Jéssica Brandão dosSilva, Camila Oliveira daTavares, Isabella ForasteiroMendes, Mayara AbudRodrigues, Ana Carolina Duarte PereiraMachado, Alice de MirandaNery, José Augusto da CostaAmadeu, Thaís PortoMoraes, Milton OzórioSarno, Euzenir NunesSchmitz, Veronica2020-03-22T18:57:54Z2020-03-22T18:57:54Z2020PACHECO, Fabiana Santos et al. Erythema Nodosum Leprosum Neutrophil Subset Expressing IL-10R1 Transmigrates into Skin Lesions and Responds to IL-10. ImmunoHorizons, v. 4, p. 47-56, Mar. 2020.2573-7732https://www.arca.fiocruz.br/handle/icict/4045510.4049/immunohorizons.1900088engAmerican Associated of ImmunologistsEritema nodoso hansenianoNeutrófilosTransmigraçãoLesões cutâneasIL-10R1Erythema Nodosum LeprosumNeutrófilosIL-10R1Skin LesionsTransmigrateErythema Nodosum Leprosum Neutrophil Subset Expressing IL-10R1 Transmigrates into Skin Lesions and Responds to IL-10info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Universiade do estado do Rio de Janeiro. Faculdade de Ciências Médicas. Laboratório de Imunopatologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Erythema nodosum leprosum (ENL) is an inflammatory complication in leprosy. Yet, the involvement of ENL neutrophils in the inflammatory response against Mycobacterium leprae remains poorly explored. Our primary aim was to investigate the utility of the surface expression of neutrophil IL-10R1 as an ENL biomarker and, secondarily, to evaluate whether leprosy or healthy M. leprae–stimulated neutrophils produce cytokines and are able to respond to IL-10. We, in this study, describe a subpopulation of circulating neutrophils of ENL patients that exclusively expressed IL-10R1, providing evidence that IL-10R1+ neutrophils are present in ENL lesions. It was also found that ENL neutrophils, but not those of nonreactional leprosy controls, were able to secret detectable levels of TNF ex vivo and the addition of IL-10 blocked TNF release. It was likewise observed that M. leprae–stimulated, healthy neutrophils expressed IL-10R1 in vitro, and ENL-linked cytokines were released by M. leprae–cultured neutrophils in vitro. Moreover, consistent with the presence of a fully functional IL-10R, the addition of IL-10 prevented the release of M. leprae–induced cytokines. Most importantly, dead M. leprae revealed its superior capacity to induce CCL4 and IL-8 in primary neutrophils over live Mycobacterium, suggesting that M. leprae may hamper the inflammatory machinery as an immune escape mechanism.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/40455/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALEuzenirSarno_MltonOsorio_etal_IOC_2020.pdfEuzenirSarno_MltonOsorio_etal_IOC_2020.pdfapplication/pdf1877531https://www.arca.fiocruz.br/bitstream/icict/40455/2/EuzenirSarno_MltonOsorio_etal_IOC_2020.pdf08b81fda97e4c6f208efa0dafaa1d248MD52TEXTEuzenirSarno_MltonOsorio_etal_IOC_2020.pdf.txtEuzenirSarno_MltonOsorio_etal_IOC_2020.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Erythema Nodosum Leprosum Neutrophil Subset Expressing IL-10R1 Transmigrates into Skin Lesions and Responds to IL-10 |
| title |
Erythema Nodosum Leprosum Neutrophil Subset Expressing IL-10R1 Transmigrates into Skin Lesions and Responds to IL-10 |
| spellingShingle |
Erythema Nodosum Leprosum Neutrophil Subset Expressing IL-10R1 Transmigrates into Skin Lesions and Responds to IL-10 Pacheco, Fabiana Santos Eritema nodoso hanseniano Neutrófilos Transmigração Lesões cutâneas IL-10R1 Erythema Nodosum Leprosum Neutrófilos IL-10R1 Skin Lesions Transmigrate |
| title_short |
Erythema Nodosum Leprosum Neutrophil Subset Expressing IL-10R1 Transmigrates into Skin Lesions and Responds to IL-10 |
| title_full |
Erythema Nodosum Leprosum Neutrophil Subset Expressing IL-10R1 Transmigrates into Skin Lesions and Responds to IL-10 |
| title_fullStr |
Erythema Nodosum Leprosum Neutrophil Subset Expressing IL-10R1 Transmigrates into Skin Lesions and Responds to IL-10 |
| title_full_unstemmed |
Erythema Nodosum Leprosum Neutrophil Subset Expressing IL-10R1 Transmigrates into Skin Lesions and Responds to IL-10 |
| title_sort |
Erythema Nodosum Leprosum Neutrophil Subset Expressing IL-10R1 Transmigrates into Skin Lesions and Responds to IL-10 |
| author |
Pacheco, Fabiana Santos |
| author_facet |
Pacheco, Fabiana Santos Prata, Rhana Berto da Silva Brandão, Sheila Santos Ferreira, Helen Rodrigues, Thais Fernanda Santos, Jéssica Brandão dos Silva, Camila Oliveira da Tavares, Isabella Forasteiro Mendes, Mayara Abud Rodrigues, Ana Carolina Duarte Pereira Machado, Alice de Miranda Nery, José Augusto da Costa Amadeu, Thaís Porto Moraes, Milton Ozório Sarno, Euzenir Nunes Schmitz, Veronica |
| author_role |
author |
| author2 |
Prata, Rhana Berto da Silva Brandão, Sheila Santos Ferreira, Helen Rodrigues, Thais Fernanda Santos, Jéssica Brandão dos Silva, Camila Oliveira da Tavares, Isabella Forasteiro Mendes, Mayara Abud Rodrigues, Ana Carolina Duarte Pereira Machado, Alice de Miranda Nery, José Augusto da Costa Amadeu, Thaís Porto Moraes, Milton Ozório Sarno, Euzenir Nunes Schmitz, Veronica |
| author2_role |
author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Pacheco, Fabiana Santos Prata, Rhana Berto da Silva Brandão, Sheila Santos Ferreira, Helen Rodrigues, Thais Fernanda Santos, Jéssica Brandão dos Silva, Camila Oliveira da Tavares, Isabella Forasteiro Mendes, Mayara Abud Rodrigues, Ana Carolina Duarte Pereira Machado, Alice de Miranda Nery, José Augusto da Costa Amadeu, Thaís Porto Moraes, Milton Ozório Sarno, Euzenir Nunes Schmitz, Veronica |
| dc.subject.other.pt_BR.fl_str_mv |
Eritema nodoso hanseniano Neutrófilos Transmigração Lesões cutâneas IL-10R1 |
| topic |
Eritema nodoso hanseniano Neutrófilos Transmigração Lesões cutâneas IL-10R1 Erythema Nodosum Leprosum Neutrófilos IL-10R1 Skin Lesions Transmigrate |
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Erythema Nodosum Leprosum Neutrófilos IL-10R1 Skin Lesions Transmigrate |
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Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. |
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2020 |
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2020-03-22T18:57:54Z |
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2020-03-22T18:57:54Z |
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2020 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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PACHECO, Fabiana Santos et al. Erythema Nodosum Leprosum Neutrophil Subset Expressing IL-10R1 Transmigrates into Skin Lesions and Responds to IL-10. ImmunoHorizons, v. 4, p. 47-56, Mar. 2020. |
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https://www.arca.fiocruz.br/handle/icict/40455 |
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2573-7732 |
| dc.identifier.doi.none.fl_str_mv |
10.4049/immunohorizons.1900088 |
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PACHECO, Fabiana Santos et al. Erythema Nodosum Leprosum Neutrophil Subset Expressing IL-10R1 Transmigrates into Skin Lesions and Responds to IL-10. ImmunoHorizons, v. 4, p. 47-56, Mar. 2020. 2573-7732 10.4049/immunohorizons.1900088 |
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American Associated of Immunologists |
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American Associated of Immunologists |
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