Leishmania braziliensis causing human disease in Northeast Brazil presents loci with genotypes in long-term equilibrium

Detalhes bibliográficos
Autor(a) principal: Silva, Juliana A.
Data de Publicação: 2022
Outros Autores: Pinheiro, Ana Isabelle, Dourado, Maria Luiza, Medina, Lilian, Queiroz, Adriano, Guimarães, Luiz Henrique, Lessa, Marcus Miranda, Lago, Ednaldo L., Machado, Paulo Roberto L., Wilson, Mary E., Carvalho, Edgar M., Schriefer, Albert
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/57301
Resumo: US National Institutes of Health (NIH). US Department of Veterans’ Affairs. Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).
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spelling Silva, Juliana A.Pinheiro, Ana IsabelleDourado, Maria LuizaMedina, LilianQueiroz, AdrianoGuimarães, Luiz HenriqueLessa, Marcus MirandaLago, Ednaldo L.Machado, Paulo Roberto L.Wilson, Mary E.Carvalho, Edgar M.Schriefer, Albert2023-03-08T19:33:49Z2023-03-08T19:33:49Z2022SILVA, Juliana A. et al. Leishmania braziliensis causing human disease in Northeast Brazil presents loci with genotypes in long-term equilibrium. PLoS Neglected Tropical Diseases, v. 16, n. 6, p. 1-19, 2022.1935-2735https://www.arca.fiocruz.br/handle/icict/5730110.1371/journal.pntd.0010390US National Institutes of Health (NIH). US Department of Veterans’ Affairs. Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Programa de Pós-graduação em Ciências da Saúde. Salvador, BA, Brasil.Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Programa de Pós-graduação em Ciências da Saúde. Salvador, BA, Brasil.Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil.Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Programa de Pós-graduação em Ciências da Saúde. Salvador, BA, Brasil.Fundação Instituto Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Salvador, BA, Brasil / Universidade Federal do Sul da Bahia. Teixeira de Freitas, BA, Brasil.Universidade Federal da Bahia. Faculdade de Medicina. Programa de Pós-graduação em Ciências da Saúde. Salvador, BA, Brasil.Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Salvador, BA, Brasil.Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Programa de Pós-graduação em Ciências da Saúde. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Salvador, BA, Brasil.Departments of Internal Medicine and Microbiology. University of Iowa and the VA Medical Center. Iowa City, Iowa, United States of America.Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Programa de Pós-graduação em Ciências da Saúde. Salvador, BA, Brasil / Fundação Instituto Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Salvador, BA, Brasil.Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Programa de Pós-graduação em Ciências da Saúde. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Salvador, BA, Brasil / Universidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Ciências da Biointeracão. Salvador, BA, Brasil.Background: Leishmaniases are neglected tropical diseases that inflict great burden to poor areas of the globe. Intense research has aimed to identify parasite genetic signatures predictive of infec tion outcomes. Consistency of diagnostic tools based on these markers would greatly bene fit from accurate understanding of Leishmania spp. population genetics. We explored two chromosomal loci to characterize a population of L. braziliensis causing human disease in Northeast Brazil. Methodology/Principal findings Two temporally distinct samples of L. braziliensis were obtained from patients attending the leishmaniasis clinic at the village of Corte de Pedra: (2008–2011) primary sample, N = 120; (1999–2001) validation sample, N = 35. Parasites were genotyped by Sanger’s sequencing of two 600 base pairs loci starting at nucleotide positions 3,074 and 425,451 of chromo somes 24 and 28, respectively. Genotypes based on haplotypes of biallelic positions in each locus were tested for several population genetic parameters as well as for geographic clustering within the region. Ample geographic overlap of genotypes at the two loci was observed as indicated by non-significant Cusick and Edward’s comparisons. No linkage dis equilibrium was detected among combinations of haplotypes for both parasite samples. Homozygous and heterozygous genotypes displayed Hardy-Weinberg equilibrium (HWE) at both loci in the two samples when straight observed and expected counts were compared by Chi-square (p>0.5). However, Bayesian statistics using one million Monte-Carlo random izations disclosed a less robust HWE for chromosome 24 genotypes, particularly in the primary sample (p = 0.04). Fixation indices (Fst) were consistently lower than 0.05 among individuals of the two samples at both tested loci, and no intra-populational structuralization could be detected using STRUCTURE software. Conclusions/Significance: These findings suggest that L. braziliensis can maintain stable populations in foci of human leishmaniasis and are capable of robust genetic recombination possibly due to events of sexual reproduction during the parasite’s lifecycle.engPublic Library of ScienceLeishmania braziliensisDoença humanaGenótiposNordeste do BrasilLeishmania braziliensisHuman diseaseGenotypesNortheast BrazilLeishmania braziliensisGenótipoLeishmania braziliensis causing human disease in Northeast Brazil presents loci with genotypes in long-term equilibriuminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALSilva, J.A. Leishmania braziliensis...2022.pdfSilva, J.A. Leishmania braziliensis...2022.pdfapplication/pdf2619218https://www.arca.fiocruz.br/bitstream/icict/57301/2/Silva%2c%20J.A.%20Leishmania%20braziliensis...2022.pdf3d90351870c5bc3b0d1ea15dc3787d70MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/57301/1/license.txt5a560609d32a3863062d77ff32785d58MD51icict/573012023-03-15 14:32:47.673oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:32:47Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en_US.fl_str_mv Leishmania braziliensis causing human disease in Northeast Brazil presents loci with genotypes in long-term equilibrium
title Leishmania braziliensis causing human disease in Northeast Brazil presents loci with genotypes in long-term equilibrium
spellingShingle Leishmania braziliensis causing human disease in Northeast Brazil presents loci with genotypes in long-term equilibrium
Silva, Juliana A.
Leishmania braziliensis
Doença humana
Genótipos
Nordeste do Brasil
Leishmania braziliensis
Human disease
Genotypes
Northeast Brazil
Leishmania braziliensis
Genótipo
title_short Leishmania braziliensis causing human disease in Northeast Brazil presents loci with genotypes in long-term equilibrium
title_full Leishmania braziliensis causing human disease in Northeast Brazil presents loci with genotypes in long-term equilibrium
title_fullStr Leishmania braziliensis causing human disease in Northeast Brazil presents loci with genotypes in long-term equilibrium
title_full_unstemmed Leishmania braziliensis causing human disease in Northeast Brazil presents loci with genotypes in long-term equilibrium
title_sort Leishmania braziliensis causing human disease in Northeast Brazil presents loci with genotypes in long-term equilibrium
author Silva, Juliana A.
author_facet Silva, Juliana A.
Pinheiro, Ana Isabelle
Dourado, Maria Luiza
Medina, Lilian
Queiroz, Adriano
Guimarães, Luiz Henrique
Lessa, Marcus Miranda
Lago, Ednaldo L.
Machado, Paulo Roberto L.
Wilson, Mary E.
Carvalho, Edgar M.
Schriefer, Albert
author_role author
author2 Pinheiro, Ana Isabelle
Dourado, Maria Luiza
Medina, Lilian
Queiroz, Adriano
Guimarães, Luiz Henrique
Lessa, Marcus Miranda
Lago, Ednaldo L.
Machado, Paulo Roberto L.
Wilson, Mary E.
Carvalho, Edgar M.
Schriefer, Albert
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, Juliana A.
Pinheiro, Ana Isabelle
Dourado, Maria Luiza
Medina, Lilian
Queiroz, Adriano
Guimarães, Luiz Henrique
Lessa, Marcus Miranda
Lago, Ednaldo L.
Machado, Paulo Roberto L.
Wilson, Mary E.
Carvalho, Edgar M.
Schriefer, Albert
dc.subject.other.en_US.fl_str_mv Leishmania braziliensis
Doença humana
Genótipos
Nordeste do Brasil
topic Leishmania braziliensis
Doença humana
Genótipos
Nordeste do Brasil
Leishmania braziliensis
Human disease
Genotypes
Northeast Brazil
Leishmania braziliensis
Genótipo
dc.subject.en.en_US.fl_str_mv Leishmania braziliensis
Human disease
Genotypes
Northeast Brazil
dc.subject.decs.en_US.fl_str_mv Leishmania braziliensis
Genótipo
description US National Institutes of Health (NIH). US Department of Veterans’ Affairs. Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).
publishDate 2022
dc.date.issued.fl_str_mv 2022
dc.date.accessioned.fl_str_mv 2023-03-08T19:33:49Z
dc.date.available.fl_str_mv 2023-03-08T19:33:49Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv SILVA, Juliana A. et al. Leishmania braziliensis causing human disease in Northeast Brazil presents loci with genotypes in long-term equilibrium. PLoS Neglected Tropical Diseases, v. 16, n. 6, p. 1-19, 2022.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/57301
dc.identifier.issn.en_US.fl_str_mv 1935-2735
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0010390
identifier_str_mv SILVA, Juliana A. et al. Leishmania braziliensis causing human disease in Northeast Brazil presents loci with genotypes in long-term equilibrium. PLoS Neglected Tropical Diseases, v. 16, n. 6, p. 1-19, 2022.
1935-2735
10.1371/journal.pntd.0010390
url https://www.arca.fiocruz.br/handle/icict/57301
dc.language.iso.fl_str_mv eng
language eng
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dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/57301/2/Silva%2c%20J.A.%20Leishmania%20braziliensis...2022.pdf
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