Characterization of Neutrophil Function in Human Cutaneous Leishmaniasis Caused by Leishmania braziliensis

Detalhes bibliográficos
Autor(a) principal: Conceição, Jacilara
Data de Publicação: 2016
Outros Autores: Davis, Richard, Carneiro, Pedro Paulo, Giudice, Angela, Muniz, Aline do Couto, Wilson, Mary E, Carvalho Filho, Edgar Marcelino, Bacellar, Olívia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1371/journal.pntd.0004715
Texto Completo: https://www.arca.fiocruz.br/handle/icict/16419
Resumo: Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil
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spelling Conceição, JacilaraDavis, RichardCarneiro, Pedro PauloGiudice, AngelaMuniz, Aline do CoutoWilson, Mary ECarvalho Filho, Edgar MarcelinoBacellar, Olívia2016-12-02T16:48:22Z2016-12-02T16:48:22Z2016CONCEIÇÃO, J. et al. Characterization of Neutrophil Function in Human Cutaneous Leishmaniasis Caused by Leishmania braziliensis. Plos Neglected Tropical Diseases, v. 10, n. 5, p. e0004715, 2016.1935-2727https://www.arca.fiocruz.br/handle/icict/1641910.1371/journal.pntd.0004715engPublic Library of ScienceLeishmaniaLeishmania BraziliensisNeutrófilosInfecçãoLeishmaniaLeishmania BraziliensisNeutrophilInfectionCharacterization of Neutrophil Function in Human Cutaneous Leishmaniasis Caused by Leishmania braziliensisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilUniversity of Iowa. Interdisciplinary Program in Immunology. Iowa City, Iowa, USAUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilUniversity of Iowa. Interdisciplinary Program in Immunology. Iowa City, Iowa, USA / University of Iowa. Department of Internal Medicine and Microbiology. Iowa City, Iowa, USA / Department of Veterans’ Affairs Medical Center. Iowa City, Iowa, USAUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais. INCT-DT (CNPq/MCT), Salvador, BA, Brasil / Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais. INCT-DT (CNPq/MCT), Salvador, BA, BrasilInfection with different Leishmania spp. protozoa can lead to a variety of clinical syndromes associated in many cases with inflammatory responses in the skin. Although macrophages harbor the majority of parasites throughout chronic infection, neutrophils are the first inflammatory cells to migrate to the site of infection. Whether neutrophils promote parasite clearance or exacerbate disease in murine models varies depending on the susceptible or resistant status of the host. Based on the hypothesis that neutrophils contribute to a systemic inflammatory state in humans with symptomatic L. braziliensis infection, we evaluated the phenotype of neutrophils from patients with cutaneous leishmaniasis (CL) during the course of L. braziliensis infection. After in vitro infection with L. braziliensis, CL patient neutrophils produced more reactive oxygen species (ROS) and higher levels of CXCL8 and CXCL9, chemokines associated with recruitment of neutrophils and Th1-type cells, than neutrophils from control healthy subjects (HS). Despite this, CL patient and HS neutrophils were equally capable of phagocytosis of L. braziliensis. There was no difference between the degree of activation of neutrophils from CL versus healthy subjects, assessed by CD66b and CD62L expression using flow cytometry. Of interest, these studies revealed that both parasite-infected and bystander neutrophils became activated during incubation with L. braziliensis. The enhanced ROS and chemokine production in neutrophils from CL patients reverted to baseline after treatment of disease. These data suggest that the circulating neutrophils during CL are not necessarily more microbicidal, but they have a more pro-inflammatory profile after parasite restimulation than neutrophils from healthy subjects.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/16419/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALConceicao J Characterization of neutrophil....pdfConceicao J Characterization of neutrophil....pdfapplication/pdf1884208https://www.arca.fiocruz.br/bitstream/icict/16419/2/Conceicao%20J%20Characterization%20of%20neutrophil....pdf31a9a40193dc5bd07afc92a65b2300b9MD52TEXTConceicao J Characterization of neutrophil....pdf.txtConceicao J Characterization of neutrophil....pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Characterization of Neutrophil Function in Human Cutaneous Leishmaniasis Caused by Leishmania braziliensis
title Characterization of Neutrophil Function in Human Cutaneous Leishmaniasis Caused by Leishmania braziliensis
spellingShingle Characterization of Neutrophil Function in Human Cutaneous Leishmaniasis Caused by Leishmania braziliensis
Conceição, Jacilara
Leishmania
Leishmania Braziliensis
Neutrófilos
Infecção
Leishmania
Leishmania Braziliensis
Neutrophil
Infection
title_short Characterization of Neutrophil Function in Human Cutaneous Leishmaniasis Caused by Leishmania braziliensis
title_full Characterization of Neutrophil Function in Human Cutaneous Leishmaniasis Caused by Leishmania braziliensis
title_fullStr Characterization of Neutrophil Function in Human Cutaneous Leishmaniasis Caused by Leishmania braziliensis
title_full_unstemmed Characterization of Neutrophil Function in Human Cutaneous Leishmaniasis Caused by Leishmania braziliensis
title_sort Characterization of Neutrophil Function in Human Cutaneous Leishmaniasis Caused by Leishmania braziliensis
author Conceição, Jacilara
author_facet Conceição, Jacilara
Davis, Richard
Carneiro, Pedro Paulo
Giudice, Angela
Muniz, Aline do Couto
Wilson, Mary E
Carvalho Filho, Edgar Marcelino
Bacellar, Olívia
author_role author
author2 Davis, Richard
Carneiro, Pedro Paulo
Giudice, Angela
Muniz, Aline do Couto
Wilson, Mary E
Carvalho Filho, Edgar Marcelino
Bacellar, Olívia
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Conceição, Jacilara
Davis, Richard
Carneiro, Pedro Paulo
Giudice, Angela
Muniz, Aline do Couto
Wilson, Mary E
Carvalho Filho, Edgar Marcelino
Bacellar, Olívia
dc.subject.other.pt_BR.fl_str_mv Leishmania
Leishmania Braziliensis
Neutrófilos
Infecção
topic Leishmania
Leishmania Braziliensis
Neutrófilos
Infecção
Leishmania
Leishmania Braziliensis
Neutrophil
Infection
dc.subject.en.pt_BR.fl_str_mv Leishmania
Leishmania Braziliensis
Neutrophil
Infection
description Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil
publishDate 2016
dc.date.accessioned.fl_str_mv 2016-12-02T16:48:22Z
dc.date.available.fl_str_mv 2016-12-02T16:48:22Z
dc.date.issued.fl_str_mv 2016
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CONCEIÇÃO, J. et al. Characterization of Neutrophil Function in Human Cutaneous Leishmaniasis Caused by Leishmania braziliensis. Plos Neglected Tropical Diseases, v. 10, n. 5, p. e0004715, 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/16419
dc.identifier.issn.pt_BR.fl_str_mv 1935-2727
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0004715
identifier_str_mv CONCEIÇÃO, J. et al. Characterization of Neutrophil Function in Human Cutaneous Leishmaniasis Caused by Leishmania braziliensis. Plos Neglected Tropical Diseases, v. 10, n. 5, p. e0004715, 2016.
1935-2727
10.1371/journal.pntd.0004715
url https://www.arca.fiocruz.br/handle/icict/16419
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/16419/1/license.txt
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