Use of MAC-ELISA for evaluation of yellow fever vaccination

Detalhes bibliográficos
Autor(a) principal: Nogueira, Rita M.
Data de Publicação: 1992
Outros Autores: Schatzmayr, Hermann G., Miagostovich, Marize. P., Cavalcanti, Silvia M. B., Carvalho, Ricardo de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/36028
Resumo: Foi avaliada a resposta imune de anticorpos IgM em vacinados contra febre amarela usando a amostra 17D empregando-se provas de captura de anticorpos IgM (MAC-ELISA) e teste de neutralização por redução de placas (PRNT). Os resulta-dos mostraram uma concordância de 97% entre ambas as provas e os autores concluem que o MAC-ELISA pode ser utilizado como um método específico e sensível para substituir o teste de neutralização para detecção de anticorpos IgM em vacinados contra febre amarela.
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spelling Nogueira, Rita M.Schatzmayr, Hermann G.Miagostovich, Marize. P.Cavalcanti, Silvia M. B.Carvalho, Ricardo de2019-10-01T13:04:48Z2019-10-01T13:04:48Z1992NOGUEIRA, Rita M. et al. Use of MAC-ELISA for evaluation of yellow fever vaccination. Revista do Instituto de Medicina Tropical de São Paulo, v. 34, n. 5, p. 447-450, set./out. 1992.0036-4665https://www.arca.fiocruz.br/handle/icict/3602810.1590/s0036-466519920005000121678-9946Foi avaliada a resposta imune de anticorpos IgM em vacinados contra febre amarela usando a amostra 17D empregando-se provas de captura de anticorpos IgM (MAC-ELISA) e teste de neutralização por redução de placas (PRNT). Os resulta-dos mostraram uma concordância de 97% entre ambas as provas e os autores concluem que o MAC-ELISA pode ser utilizado como um método específico e sensível para substituir o teste de neutralização para detecção de anticorpos IgM em vacinados contra febre amarela.An evaluation of the IgM antibody immune response against yellow fever using strain 17D was carried out by MAC-ELISA and PRNT. The results showed an agreement of 97% between both tests and the authors conclude that MAC-ELISA can be used as a specific and sensitive assay to replace the PRNT for detecting yellow fever antibodies in human sera, after vaccination programs.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.engInstituto de Medicina Tropical de São PauloFebre amarelaVacinaçãoMAC-ELISAYellow fever vaccinatedMAC-ELISAPRNTUse of MAC-ELISA for evaluation of yellow fever vaccinationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/36028/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALRitaRibeiro_MarizeMiagostovich_etal_IOC_1992.pdfRitaRibeiro_MarizeMiagostovich_etal_IOC_1992.pdfapplication/pdf246579https://www.arca.fiocruz.br/bitstream/icict/36028/2/RitaRibeiro_MarizeMiagostovich_etal_IOC_1992.pdf1e7453dc804e906c9e27754ae4e3015dMD52TEXTRitaRibeiro_MarizeMiagostovich_etal_IOC_1992.pdf.txtRitaRibeiro_MarizeMiagostovich_etal_IOC_1992.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Use of MAC-ELISA for evaluation of yellow fever vaccination
title Use of MAC-ELISA for evaluation of yellow fever vaccination
spellingShingle Use of MAC-ELISA for evaluation of yellow fever vaccination
Nogueira, Rita M.
Febre amarela
Vacinação
MAC-ELISA
Yellow fever vaccinated
MAC-ELISA
PRNT
title_short Use of MAC-ELISA for evaluation of yellow fever vaccination
title_full Use of MAC-ELISA for evaluation of yellow fever vaccination
title_fullStr Use of MAC-ELISA for evaluation of yellow fever vaccination
title_full_unstemmed Use of MAC-ELISA for evaluation of yellow fever vaccination
title_sort Use of MAC-ELISA for evaluation of yellow fever vaccination
author Nogueira, Rita M.
author_facet Nogueira, Rita M.
Schatzmayr, Hermann G.
Miagostovich, Marize. P.
Cavalcanti, Silvia M. B.
Carvalho, Ricardo de
author_role author
author2 Schatzmayr, Hermann G.
Miagostovich, Marize. P.
Cavalcanti, Silvia M. B.
Carvalho, Ricardo de
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Nogueira, Rita M.
Schatzmayr, Hermann G.
Miagostovich, Marize. P.
Cavalcanti, Silvia M. B.
Carvalho, Ricardo de
dc.subject.other.pt_BR.fl_str_mv Febre amarela
Vacinação
MAC-ELISA
topic Febre amarela
Vacinação
MAC-ELISA
Yellow fever vaccinated
MAC-ELISA
PRNT
dc.subject.en.pt_BR.fl_str_mv Yellow fever vaccinated
MAC-ELISA
PRNT
description Foi avaliada a resposta imune de anticorpos IgM em vacinados contra febre amarela usando a amostra 17D empregando-se provas de captura de anticorpos IgM (MAC-ELISA) e teste de neutralização por redução de placas (PRNT). Os resulta-dos mostraram uma concordância de 97% entre ambas as provas e os autores concluem que o MAC-ELISA pode ser utilizado como um método específico e sensível para substituir o teste de neutralização para detecção de anticorpos IgM em vacinados contra febre amarela.
publishDate 1992
dc.date.issued.fl_str_mv 1992
dc.date.accessioned.fl_str_mv 2019-10-01T13:04:48Z
dc.date.available.fl_str_mv 2019-10-01T13:04:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv NOGUEIRA, Rita M. et al. Use of MAC-ELISA for evaluation of yellow fever vaccination. Revista do Instituto de Medicina Tropical de São Paulo, v. 34, n. 5, p. 447-450, set./out. 1992.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/36028
dc.identifier.issn.pt_BR.fl_str_mv 0036-4665
dc.identifier.doi.none.fl_str_mv 10.1590/s0036-46651992000500012
dc.identifier.eissn.none.fl_str_mv 1678-9946
identifier_str_mv NOGUEIRA, Rita M. et al. Use of MAC-ELISA for evaluation of yellow fever vaccination. Revista do Instituto de Medicina Tropical de São Paulo, v. 34, n. 5, p. 447-450, set./out. 1992.
0036-4665
10.1590/s0036-46651992000500012
1678-9946
url https://www.arca.fiocruz.br/handle/icict/36028
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dc.publisher.none.fl_str_mv Instituto de Medicina Tropical de São Paulo
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