Molecular Characterization of Noroviruses and HBGA from Infected Quilombola Children in Espirito Santo State, Brazil
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2013 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/11872 |
Resumo: | Universidade Federal do Espírito Santo. Departamento de Ciências da Saúde. São Mateus, ES, Brasil / Universidade Federal do Espírito Santo. Programa de Pós-Graduação em Doenças Infecciosas. Vitória, ES, Brasil. |
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Vicentini, FernandoDenadai, WilsonGomes, Yohanna MayelleRose, Tatiana L.Ferreira, Mônica S. R.Moulac-Vaidye, Beatrice LePendu, Jacques LeLeite, José Paulo GagliardiMiagostovich, Marize PereiraSpano, Liliana Cruz2015-10-02T12:45:07Z2015-10-02T12:45:07Z2013VICENTINI, Fernando; et al. Molecular Characterization of Noroviruses and HBGA from Infected Quilombola Children in Espirito Santo State, Brazil. Plos One, v.8, n.7, e69348, 9p, 2013.https://www.arca.fiocruz.br/handle/icict/1187210.1371/journal.pone.0069348engPlos OneQuilombolaEspírito SantoNoroviruses (NoV)Quilombola ChildrenChildrenNorovirusCriançasMolecular Characterization of Noroviruses and HBGA from Infected Quilombola Children in Espirito Santo State, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Espírito Santo. Departamento de Ciências da Saúde. São Mateus, ES, Brasil / Universidade Federal do Espírito Santo. Programa de Pós-Graduação em Doenças Infecciosas. Vitória, ES, Brasil.Universidade Federal do Espírito Santo. Departamento de Ciências da Saúde. São Mateus, ES, Brasil.Universidade Federal do Espírito Santo. Departamento de Patologia. Vitória, ES, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.Institut National de la Santé et de la Recherche Médicale. Nantes, France / Centre de Recherche en Cancérologie.Nantes, France / Université de Nantes. Nantes, France.Institut National de la Santé et de la Recherche Médicale. Nantes, France / Centre de Recherche en Cancérologie.Nantes, France / Université de Nantes. Nantes, France.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.Universidade Federal do Espírito Santo. Departamento de Patologia. Vitória, ES, Brasil.Noroviruses (NoV) are the main etiological agents of gastroenteritis outbreaks worldwide and susceptibility to NoV infection has been related to the histo-blood group antigen (HBGA). This study aimed to determine the prevalence of NoV strains and to evaluate the HBGA phenotype and genotype of children from semi-isolated Quilombola communities, descendents of black slaves in Brazil. A total of 397 children up to eleven years old, with and without diarrhea, from Quilombola Communities in the Espirito Santo State, Brazil, were investigated for the presence of NoV from August 2007 to September 2009. Feces were collected from all the children, and blood from the NoV positive children. NoV was screened by reverse transcription-PCR with primers for the RNA-dependent RNA polymerase region; genogroup was determined by PCR with primers for the C and D regions and genotyped by sequencing. HBGA phenotype was performed by gel-spinning and FUT2 and FUT3 were analyzed by PCR or sequencing analysis. NoV were detected in 9.2% (12/131) of diarrheic and 1.5% (4/266) of non-diarrheic children (p,0.05, Fisher’s exact test). GI and GII genogroups were present in 12.5% and 87.5% of the samples, respectively. The following genotypes were characterized: GII.4 (25%), GII.12 (25%), GII.6 (12.5%) and GI.1 (6.3%), GI.3 (12.5%) and GI.4 (6.3%). Children infected with NoV showed the A (n = 6), O (n = 6), and B (n = 2) HBGA phenotypes, and 13 of them were classified as secretors (Se) and one as a non secretor (se). Mutations of Se40, 171,216,357,428,739,960 were found for the FUT2 gene and mutations of Le59, 202, 314 for the FUT3 gene. The only se child was infected by NoV GI, whereas the Se children were indiscriminately infected by GI or GII. This study showed rates of NoV infection in symptomatic and asymptomatic Quilombola children consistent with other studies. However, children under 12 months were seven times more affected than those between 1 and 5 years old. GII.12 was as frequent as GII.4 and GI.1 and GI.4 were described for the first time in Brazil. Owing to the small number of cases studied, no clear pattern of susceptibility and/or HBGA resistance could be inferred.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/11872/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALmonica_ferreira_etal_IOC_2013.pdfapplication/pdf1638011https://www.arca.fiocruz.br/bitstream/icict/11872/2/monica_ferreira_etal_IOC_2013.pdf2d31083792c82cb4b2cbc9b37d72d041MD52TEXTmonica_ferreira_etal_IOC_2013.pdf.txtmonica_ferreira_etal_IOC_2013.pdf.txtExtracted texttext/plain46231https://www.arca.fiocruz.br/bitstream/icict/11872/3/monica_ferreira_etal_IOC_2013.pdf.txt1041de0b74ae58fd0c532aed73f1734eMD53icict/118722022-06-24 13:12:35.679oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T16:12:35Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Molecular Characterization of Noroviruses and HBGA from Infected Quilombola Children in Espirito Santo State, Brazil |
| title |
Molecular Characterization of Noroviruses and HBGA from Infected Quilombola Children in Espirito Santo State, Brazil |
| spellingShingle |
Molecular Characterization of Noroviruses and HBGA from Infected Quilombola Children in Espirito Santo State, Brazil Vicentini, Fernando Quilombola Espírito Santo Noroviruses (NoV) Quilombola Children Children Norovirus Crianças |
| title_short |
Molecular Characterization of Noroviruses and HBGA from Infected Quilombola Children in Espirito Santo State, Brazil |
| title_full |
Molecular Characterization of Noroviruses and HBGA from Infected Quilombola Children in Espirito Santo State, Brazil |
| title_fullStr |
Molecular Characterization of Noroviruses and HBGA from Infected Quilombola Children in Espirito Santo State, Brazil |
| title_full_unstemmed |
Molecular Characterization of Noroviruses and HBGA from Infected Quilombola Children in Espirito Santo State, Brazil |
| title_sort |
Molecular Characterization of Noroviruses and HBGA from Infected Quilombola Children in Espirito Santo State, Brazil |
| author |
Vicentini, Fernando |
| author_facet |
Vicentini, Fernando Denadai, Wilson Gomes, Yohanna Mayelle Rose, Tatiana L. Ferreira, Mônica S. R. Moulac-Vaidye, Beatrice Le Pendu, Jacques Le Leite, José Paulo Gagliardi Miagostovich, Marize Pereira Spano, Liliana Cruz |
| author_role |
author |
| author2 |
Denadai, Wilson Gomes, Yohanna Mayelle Rose, Tatiana L. Ferreira, Mônica S. R. Moulac-Vaidye, Beatrice Le Pendu, Jacques Le Leite, José Paulo Gagliardi Miagostovich, Marize Pereira Spano, Liliana Cruz |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Vicentini, Fernando Denadai, Wilson Gomes, Yohanna Mayelle Rose, Tatiana L. Ferreira, Mônica S. R. Moulac-Vaidye, Beatrice Le Pendu, Jacques Le Leite, José Paulo Gagliardi Miagostovich, Marize Pereira Spano, Liliana Cruz |
| dc.subject.other.pt_BR.fl_str_mv |
Quilombola Espírito Santo |
| topic |
Quilombola Espírito Santo Noroviruses (NoV) Quilombola Children Children Norovirus Crianças |
| dc.subject.en.pt_BR.fl_str_mv |
Noroviruses (NoV) Quilombola Children Children |
| dc.subject.decs.pt_BR.fl_str_mv |
Norovirus Crianças |
| description |
Universidade Federal do Espírito Santo. Departamento de Ciências da Saúde. São Mateus, ES, Brasil / Universidade Federal do Espírito Santo. Programa de Pós-Graduação em Doenças Infecciosas. Vitória, ES, Brasil. |
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2013 |
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2013 |
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2015-10-02T12:45:07Z |
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2015-10-02T12:45:07Z |
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info:eu-repo/semantics/article |
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VICENTINI, Fernando; et al. Molecular Characterization of Noroviruses and HBGA from Infected Quilombola Children in Espirito Santo State, Brazil. Plos One, v.8, n.7, e69348, 9p, 2013. |
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https://www.arca.fiocruz.br/handle/icict/11872 |
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10.1371/journal.pone.0069348 |
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VICENTINI, Fernando; et al. Molecular Characterization of Noroviruses and HBGA from Infected Quilombola Children in Espirito Santo State, Brazil. Plos One, v.8, n.7, e69348, 9p, 2013. 10.1371/journal.pone.0069348 |
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