Caliciviruses in hospitalized children, São Luís, Maranhão, 1997-1999: detection of norovirus GII.12
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300724 |
Resumo: | ABSTRACT Gastroenteritis is one of the most common diseases during childhood, with norovirus (NoV) and sapovirus (SaV) being two of its main causes. This study reports for the first time the incidence of these viruses in hospitalized children with and without gastroenteritis in São Luís, Maranhão. A total of 136 fecal samples were tested by enzyme immunoassays (EIA) for the detection of NoV and by reverse transcription-polymerase chain reaction (RT-PCR) for detection of both NoV and SaV. Positive samples for both agents were subjected to sequencing. The overall frequency of NoV as detected by EIA and RT-PCR was 17.6% (24/136) and 32.6% (15/46), respectively in diarrheic patients and 10.0% (9/90) in non-diarrheic patients (p < 0.01). Of the diarrheic patients, 17% had fever, vomiting and anorexia, and 13% developed fever, vomiting and abdominal pain. Of the 24 NoV-positive samples, 50% (12/24) were sequenced and classified as genotypes GII.3 (n = 1), GII.4 (6), GII.5 (1), GII.7 (2), GII.12 (1) and GII.16 (1). SaV frequency was 9.8% (11/112), with 22.6% (7/31) in diarrheic patients and 4.9% (4/81) in nondiarrheic (p = 0.04) ones. In diarrheic cases, 27.3% had fever, vomiting and anorexia, whereas 18.2% had fever, anorexia and abdominal pain. One SaV-positive sample was sequenced and classified as GII.1. These results show a high genetic diversity of NoV and higher prevalence of NoV compared to SaV. Our data highlight the importance of NoV and SaV as enteropathogens in São Luís, Maranhão. |
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Caliciviruses in hospitalized children, São Luís, Maranhão, 1997-1999: detection of norovirus GII.12NorovirusSapovirusGastroenteritisChildrenABSTRACT Gastroenteritis is one of the most common diseases during childhood, with norovirus (NoV) and sapovirus (SaV) being two of its main causes. This study reports for the first time the incidence of these viruses in hospitalized children with and without gastroenteritis in São Luís, Maranhão. A total of 136 fecal samples were tested by enzyme immunoassays (EIA) for the detection of NoV and by reverse transcription-polymerase chain reaction (RT-PCR) for detection of both NoV and SaV. Positive samples for both agents were subjected to sequencing. The overall frequency of NoV as detected by EIA and RT-PCR was 17.6% (24/136) and 32.6% (15/46), respectively in diarrheic patients and 10.0% (9/90) in non-diarrheic patients (p < 0.01). Of the diarrheic patients, 17% had fever, vomiting and anorexia, and 13% developed fever, vomiting and abdominal pain. Of the 24 NoV-positive samples, 50% (12/24) were sequenced and classified as genotypes GII.3 (n = 1), GII.4 (6), GII.5 (1), GII.7 (2), GII.12 (1) and GII.16 (1). SaV frequency was 9.8% (11/112), with 22.6% (7/31) in diarrheic patients and 4.9% (4/81) in nondiarrheic (p = 0.04) ones. In diarrheic cases, 27.3% had fever, vomiting and anorexia, whereas 18.2% had fever, anorexia and abdominal pain. One SaV-positive sample was sequenced and classified as GII.1. These results show a high genetic diversity of NoV and higher prevalence of NoV compared to SaV. Our data highlight the importance of NoV and SaV as enteropathogens in São Luís, Maranhão.Sociedade Brasileira de Microbiologia2016-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300724Brazilian Journal of Microbiology v.47 n.3 2016reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1016/j.bjm.2016.04.008info:eu-repo/semantics/openAccessPortal,Thayara MoraisSiqueira,Jones Anderson MonteiroCosta,Larissa Cristina Prado das NevesLima,Ian Carlos Gomes deLucena,Maria Silvia Sousa deBandeira,Renato da SilvaLinhares,Alexandre da CostaLuz,Claudia Regina Nunes Eloi daGabbay,Yvone BenchimolResque,Hugo Reiseng2016-08-02T00:00:00Zoai:scielo:S1517-83822016000300724Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2016-08-02T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Caliciviruses in hospitalized children, São Luís, Maranhão, 1997-1999: detection of norovirus GII.12 |
title |
Caliciviruses in hospitalized children, São Luís, Maranhão, 1997-1999: detection of norovirus GII.12 |
spellingShingle |
Caliciviruses in hospitalized children, São Luís, Maranhão, 1997-1999: detection of norovirus GII.12 Portal,Thayara Morais Norovirus Sapovirus Gastroenteritis Children |
title_short |
Caliciviruses in hospitalized children, São Luís, Maranhão, 1997-1999: detection of norovirus GII.12 |
title_full |
Caliciviruses in hospitalized children, São Luís, Maranhão, 1997-1999: detection of norovirus GII.12 |
title_fullStr |
Caliciviruses in hospitalized children, São Luís, Maranhão, 1997-1999: detection of norovirus GII.12 |
title_full_unstemmed |
Caliciviruses in hospitalized children, São Luís, Maranhão, 1997-1999: detection of norovirus GII.12 |
title_sort |
Caliciviruses in hospitalized children, São Luís, Maranhão, 1997-1999: detection of norovirus GII.12 |
author |
Portal,Thayara Morais |
author_facet |
Portal,Thayara Morais Siqueira,Jones Anderson Monteiro Costa,Larissa Cristina Prado das Neves Lima,Ian Carlos Gomes de Lucena,Maria Silvia Sousa de Bandeira,Renato da Silva Linhares,Alexandre da Costa Luz,Claudia Regina Nunes Eloi da Gabbay,Yvone Benchimol Resque,Hugo Reis |
author_role |
author |
author2 |
Siqueira,Jones Anderson Monteiro Costa,Larissa Cristina Prado das Neves Lima,Ian Carlos Gomes de Lucena,Maria Silvia Sousa de Bandeira,Renato da Silva Linhares,Alexandre da Costa Luz,Claudia Regina Nunes Eloi da Gabbay,Yvone Benchimol Resque,Hugo Reis |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Portal,Thayara Morais Siqueira,Jones Anderson Monteiro Costa,Larissa Cristina Prado das Neves Lima,Ian Carlos Gomes de Lucena,Maria Silvia Sousa de Bandeira,Renato da Silva Linhares,Alexandre da Costa Luz,Claudia Regina Nunes Eloi da Gabbay,Yvone Benchimol Resque,Hugo Reis |
dc.subject.por.fl_str_mv |
Norovirus Sapovirus Gastroenteritis Children |
topic |
Norovirus Sapovirus Gastroenteritis Children |
description |
ABSTRACT Gastroenteritis is one of the most common diseases during childhood, with norovirus (NoV) and sapovirus (SaV) being two of its main causes. This study reports for the first time the incidence of these viruses in hospitalized children with and without gastroenteritis in São Luís, Maranhão. A total of 136 fecal samples were tested by enzyme immunoassays (EIA) for the detection of NoV and by reverse transcription-polymerase chain reaction (RT-PCR) for detection of both NoV and SaV. Positive samples for both agents were subjected to sequencing. The overall frequency of NoV as detected by EIA and RT-PCR was 17.6% (24/136) and 32.6% (15/46), respectively in diarrheic patients and 10.0% (9/90) in non-diarrheic patients (p < 0.01). Of the diarrheic patients, 17% had fever, vomiting and anorexia, and 13% developed fever, vomiting and abdominal pain. Of the 24 NoV-positive samples, 50% (12/24) were sequenced and classified as genotypes GII.3 (n = 1), GII.4 (6), GII.5 (1), GII.7 (2), GII.12 (1) and GII.16 (1). SaV frequency was 9.8% (11/112), with 22.6% (7/31) in diarrheic patients and 4.9% (4/81) in nondiarrheic (p = 0.04) ones. In diarrheic cases, 27.3% had fever, vomiting and anorexia, whereas 18.2% had fever, anorexia and abdominal pain. One SaV-positive sample was sequenced and classified as GII.1. These results show a high genetic diversity of NoV and higher prevalence of NoV compared to SaV. Our data highlight the importance of NoV and SaV as enteropathogens in São Luís, Maranhão. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300724 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300724 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1016/j.bjm.2016.04.008 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.47 n.3 2016 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
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1752122208457588736 |