Endovascular infections caused by methicillin-resistant Staphylococcus aureus are linked to clonal complex-specific alterations in binding and invasion domains of fibronectin-binding protein A as well as the occurrence of fnbB
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2015 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/21054 |
Resumo: | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil |
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Xiong, Yan Q.Sharma-Kuinkel, Batu K.Casillas-Ituarte, Nadia N.Fowler, Vance G.Rude, ThomasDiBartola, Alex C.Lins, Roberto D.Abdel-Hady, WessamLower, Steven K.Bayer, Arnold S.2017-09-18T19:57:45Z2017-09-18T19:57:45Z2015XIONG, Y. Q. et al. Endovascular infections caused by methicillin-resistant Staphylococcus aureus are linked to clonal complex-specific alterations in binding and invasion domains of fibronectin-binding protein A as well as the occurrence of fnbB. Infection and Immunity, v. 83, n. 12, p. 4772–4780, dez. 2015.1098-5522https://www.arca.fiocruz.br/handle/icict/2105410.1128/IAI.01074-15porAdesinas BacterianasBacteriemiaFibronectinasStaphylococcus aureus Resistente à MeticilinaIsoformas de ProteínasAdhesins, BacterialAmino Acid SequenceBacteremiaBinding SitesBlood VesselsClone CellsFibronectinsGene ExpressionHost-Pathogen InteractionsHumansImmobilized ProteinsMethicillin-Resistant Staphylococcus aureusMolecular Sequence DataPolymorphism, GeneticProtein BindingProtein IsoformsProtein Structure, TertiaryProtein Structure, TertiaryStaphylococcal InfectionsAdesinas BacterianasBacteremiaSequência de AminoácidosSítios de LigaçãoVasos SanguíneosCélulas ClonaiFibronectinasExpressão GênicaInterações Hospedeiro-PatógenoHumanosProteínas ImobilizadasStaphylococcus aureus Resistente à MeticilinaDados de Sequência MolecularPolimorfismo GenéticoLigação ProteicaIsoformas de ProteínasEstrutura Terciária de ProteínaEndovascular infections caused by methicillin-resistant Staphylococcus aureus are linked to clonal complex-specific alterations in binding and invasion domains of fibronectin-binding protein A as well as the occurrence of fnbBinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, BrasilEndovascular infections caused by Staphylococcus aureus involve interactions with fibronectin present as extracellular matrix or surface ligand on host cells. We examined the expression, structure, and binding activity of the two major S. aureus fibronectin-binding proteins (FnBPA, FnBPB) in 10 distinct, methicillin-resistant clinical isolates from patients with either persistent or resolving bacteremia. The persistent bacteremia isolates (n = 5) formed significantly stronger bonds with immobilized fibronectin as determined by dynamic binding measurements performed with atomic force microscopy. Several notable differences were also observed when the results were grouped by clonal complex 5 (CC5) strains (n = 5) versus CC45 strains (n = 5). Fibronectin-binding receptors on CC5 formed stronger bonds with immobilized fibronectin (P < 0.001). The fnbA gene was expressed at higher levels in CC45, whereas fnbB was found in only CC5 isolates. The fnbB gene was not sequenced because all CC45 isolates lacked this gene. Instead, comparisons were made for fnbA, which was present in all 10 isolates. Sequencing of fnbA revealed discrete differences within high-affinity, fibronectin-binding repeats (FnBRs) of FnBPA that included (i) 5-amino-acid polymorphisms in FnBR-9, FnBR-10, and FnBR-11 involving charged or polar side chains, (ii) an extra, 38-amino-acid repeat inserted between FnBR-9 and FnBR-10 exclusively seen in CC45 isolates, and (iii) CC5 isolates had the SVDFEED epitope in FnBR-11 (a sequence shown to be essential for fibronectin binding), while this sequence was replaced in all CC45 isolates with GIDFVED (a motif known to favor host cell invasion at the cost of reduced fibronectin binding). These complementary sequence and binding data suggest that differences in fnbA and fnbB, particularly polymorphisms and duplications in FnBPA, give S. aureus two distinct advantages in human endovascular infections: (i) FnBPs similar to that of CC5 enhance ligand binding and foster initiation of disease, and (ii) CC45-like FnBPs promote cell invasion, a key attribute in persistent endovascular infections.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/21054/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINAL26416903 2015 xio-end.oa.pdf26416903 2015 xio-end.oa.pdfapplication/pdf1395222https://www.arca.fiocruz.br/bitstream/icict/21054/2/26416903%202015%20xio-end.oa.pdf6bb19e6e755efd7c08aa16c441249ba5MD52TEXT26416903 2015 xio-end.oa.pdf.txt26416903 2015 xio-end.oa.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Endovascular infections caused by methicillin-resistant Staphylococcus aureus are linked to clonal complex-specific alterations in binding and invasion domains of fibronectin-binding protein A as well as the occurrence of fnbB |
| title |
Endovascular infections caused by methicillin-resistant Staphylococcus aureus are linked to clonal complex-specific alterations in binding and invasion domains of fibronectin-binding protein A as well as the occurrence of fnbB |
| spellingShingle |
Endovascular infections caused by methicillin-resistant Staphylococcus aureus are linked to clonal complex-specific alterations in binding and invasion domains of fibronectin-binding protein A as well as the occurrence of fnbB Xiong, Yan Q. Adesinas Bacterianas Bacteriemia Fibronectinas Staphylococcus aureus Resistente à Meticilina Isoformas de Proteínas Adhesins, Bacterial Amino Acid Sequence Bacteremia Binding Sites Blood Vessels Clone Cells Fibronectins Gene Expression Host-Pathogen Interactions Humans Immobilized Proteins Methicillin-Resistant Staphylococcus aureus Molecular Sequence Data Polymorphism, Genetic Protein Binding Protein Isoforms Protein Structure, Tertiary Protein Structure, Tertiary Staphylococcal Infections Adesinas Bacterianas Bacteremia Sequência de Aminoácidos Sítios de Ligação Vasos Sanguíneos Células Clonai Fibronectinas Expressão Gênica Interações Hospedeiro-Patógeno Humanos Proteínas Imobilizadas Staphylococcus aureus Resistente à Meticilina Dados de Sequência Molecular Polimorfismo Genético Ligação Proteica Isoformas de Proteínas Estrutura Terciária de Proteína |
| title_short |
Endovascular infections caused by methicillin-resistant Staphylococcus aureus are linked to clonal complex-specific alterations in binding and invasion domains of fibronectin-binding protein A as well as the occurrence of fnbB |
| title_full |
Endovascular infections caused by methicillin-resistant Staphylococcus aureus are linked to clonal complex-specific alterations in binding and invasion domains of fibronectin-binding protein A as well as the occurrence of fnbB |
| title_fullStr |
Endovascular infections caused by methicillin-resistant Staphylococcus aureus are linked to clonal complex-specific alterations in binding and invasion domains of fibronectin-binding protein A as well as the occurrence of fnbB |
| title_full_unstemmed |
Endovascular infections caused by methicillin-resistant Staphylococcus aureus are linked to clonal complex-specific alterations in binding and invasion domains of fibronectin-binding protein A as well as the occurrence of fnbB |
| title_sort |
Endovascular infections caused by methicillin-resistant Staphylococcus aureus are linked to clonal complex-specific alterations in binding and invasion domains of fibronectin-binding protein A as well as the occurrence of fnbB |
| author |
Xiong, Yan Q. |
| author_facet |
Xiong, Yan Q. Sharma-Kuinkel, Batu K. Casillas-Ituarte, Nadia N. Fowler, Vance G. Rude, Thomas DiBartola, Alex C. Lins, Roberto D. Abdel-Hady, Wessam Lower, Steven K. Bayer, Arnold S. |
| author_role |
author |
| author2 |
Sharma-Kuinkel, Batu K. Casillas-Ituarte, Nadia N. Fowler, Vance G. Rude, Thomas DiBartola, Alex C. Lins, Roberto D. Abdel-Hady, Wessam Lower, Steven K. Bayer, Arnold S. |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Xiong, Yan Q. Sharma-Kuinkel, Batu K. Casillas-Ituarte, Nadia N. Fowler, Vance G. Rude, Thomas DiBartola, Alex C. Lins, Roberto D. Abdel-Hady, Wessam Lower, Steven K. Bayer, Arnold S. |
| dc.subject.other.pt_BR.fl_str_mv |
Adesinas Bacterianas Bacteriemia Fibronectinas Staphylococcus aureus Resistente à Meticilina Isoformas de Proteínas |
| topic |
Adesinas Bacterianas Bacteriemia Fibronectinas Staphylococcus aureus Resistente à Meticilina Isoformas de Proteínas Adhesins, Bacterial Amino Acid Sequence Bacteremia Binding Sites Blood Vessels Clone Cells Fibronectins Gene Expression Host-Pathogen Interactions Humans Immobilized Proteins Methicillin-Resistant Staphylococcus aureus Molecular Sequence Data Polymorphism, Genetic Protein Binding Protein Isoforms Protein Structure, Tertiary Protein Structure, Tertiary Staphylococcal Infections Adesinas Bacterianas Bacteremia Sequência de Aminoácidos Sítios de Ligação Vasos Sanguíneos Células Clonai Fibronectinas Expressão Gênica Interações Hospedeiro-Patógeno Humanos Proteínas Imobilizadas Staphylococcus aureus Resistente à Meticilina Dados de Sequência Molecular Polimorfismo Genético Ligação Proteica Isoformas de Proteínas Estrutura Terciária de Proteína |
| dc.subject.en.pt_BR.fl_str_mv |
Adhesins, Bacterial Amino Acid Sequence Bacteremia Binding Sites Blood Vessels Clone Cells Fibronectins Gene Expression Host-Pathogen Interactions Humans Immobilized Proteins Methicillin-Resistant Staphylococcus aureus Molecular Sequence Data Polymorphism, Genetic Protein Binding Protein Isoforms Protein Structure, Tertiary Protein Structure, Tertiary Staphylococcal Infections |
| dc.subject.decs.pt_BR.fl_str_mv |
Adesinas Bacterianas Bacteremia Sequência de Aminoácidos Sítios de Ligação Vasos Sanguíneos Células Clonai Fibronectinas Expressão Gênica Interações Hospedeiro-Patógeno Humanos Proteínas Imobilizadas Staphylococcus aureus Resistente à Meticilina Dados de Sequência Molecular Polimorfismo Genético Ligação Proteica Isoformas de Proteínas Estrutura Terciária de Proteína |
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Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil |
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2015 |
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2015 |
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2017-09-18T19:57:45Z |
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2017-09-18T19:57:45Z |
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XIONG, Y. Q. et al. Endovascular infections caused by methicillin-resistant Staphylococcus aureus are linked to clonal complex-specific alterations in binding and invasion domains of fibronectin-binding protein A as well as the occurrence of fnbB. Infection and Immunity, v. 83, n. 12, p. 4772–4780, dez. 2015. |
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https://www.arca.fiocruz.br/handle/icict/21054 |
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1098-5522 |
| dc.identifier.eissn.none.fl_str_mv |
10.1128/IAI.01074-15 |
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XIONG, Y. Q. et al. Endovascular infections caused by methicillin-resistant Staphylococcus aureus are linked to clonal complex-specific alterations in binding and invasion domains of fibronectin-binding protein A as well as the occurrence of fnbB. Infection and Immunity, v. 83, n. 12, p. 4772–4780, dez. 2015. 1098-5522 10.1128/IAI.01074-15 |
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