Candidatus Liberibacter americanus induces significant reprogramming of the transcriptome of the susceptible citrus genotype

Detalhes bibliográficos
Autor(a) principal: Mafra, Valéria
Data de Publicação: 2013
Outros Autores: Martins, Polyana K., Francisco, Carolina S., Ribeiro-Alves, Marcelo, Freitas-Astúa, Juliana, Machado, Marcos A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/35651
Resumo: Centro de Citricultura Sylvio Moreira. Cordeirópolis, SP, Brasil / Instituto Agronômico de Campinas. Campinas, SP, Brasil / Universidade Estadual de Campinas. Campinas, SP, Brasil.
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spelling Mafra, ValériaMartins, Polyana K.Francisco, Carolina S.Ribeiro-Alves, MarceloFreitas-Astúa, JulianaMachado, Marcos A.2019-09-18T12:29:54Z2019-09-18T12:29:54Z2013MAFRA, Valéria et al. Candidatus Liberibacter americanus induces significant reprogramming of the transcriptome of the susceptible citrus genotype. BMC Genomics, v. 14, p. 1-15, 2013.1471-2164https://www.arca.fiocruz.br/handle/icict/3565110.1186/1471-2164-14-2471471-2164engBMCCandidatus Liberibacter americanus induces significant reprogramming of the transcriptome of the susceptible citrus genotypeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCentro de Citricultura Sylvio Moreira. Cordeirópolis, SP, Brasil / Instituto Agronômico de Campinas. Campinas, SP, Brasil / Universidade Estadual de Campinas. Campinas, SP, Brasil.Centro de Citricultura Sylvio Moreira. Cordeirópolis, SP, Brasil / Instituto Agronômico de Campinas. Campinas, SP, Brasil.Centro de Citricultura Sylvio Moreira. Cordeirópolis, SP, Brasil / Instituto Agronômico de Campinas. Campinas, SP, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Pesquisa em Farmacogenética. Rio de Janeiro, RJ, Brasil.Centro de Citricultura Sylvio Moreira. Cordeirópolis, SP, Brasil / Instituto Agronômico de Campinas. Campinas, SP, Brasil / Embrapa Cassava & Fruits. Cruz das Almas, BA, Brasil.Centro de Citricultura Sylvio Moreira. Cordeirópolis, SP, Brasil / Instituto Agronômico de Campinas. Campinas, SP, Brasil.Background: Citrus huanglongbing (HLB) disease is caused by endogenous, phloem-restricted, Gram negative, uncultured bacteria named Candidatus Liberibacter africanus (CaLaf), Ca. L. asiaticus (CaLas), and Ca. L. americanus (CaLam), depending on the continent where the bacteria were first detected. The Asian citrus psyllid vector, Diaphorina citri, transmits CaLas and CaLam and both Liberibacter species are present in Brazil. Several studies of the transcriptional response of citrus plants manifesting HLB symptoms have been reported, but only for CaLas infection. This study evaluated the transcriptional reprogramming of a susceptible genotype of sweet orange challenged with CaLam, using a customized 385K microarray containing approximately 32,000 unigene transcripts. We analyzed global changes in gene expression of CaLam-infected leaves of sweet orange during the symptomatic stage of infection and compared the results with previously published microarray studies that used CaLas-infected plants. Twenty candidate genes were selected to validate the expression profiles in symptomatic and asymptomatic PCR-positive leaves infected with CaLas or CaLam. Results: The microarray analysis identified 633 differentially expressed genes during the symptomatic stage of CaLam infection. Among them, 418 (66%) were upregulated and 215 (34%) were down regulated. Five hundred and fourteen genes (81%) were orthologs of genes from Arabidopsis thaliana. Gene set enrichment analysis (GSEA) revealed that several of the transcripts encoded transporters associated with the endomembrane system, especially zinc transport. Among the most biologically relevant gene transcripts in GSEA were those related to signaling, metabolism and/or stimulus to hormones, genes responding to stress and pathogenesis, biosynthesis of secondary metabolites, oxidative stress and transcription factors belonging to different families. Real time PCR of 20 candidate genes validated the expression pattern of some genes in symptomatic and asymptomatic leaves infected with CaLam or CaLas. Conclusions: Many gene transcripts and biological processes are significantly altered upon CaLam infection. Some of them had been identified in response to CaLas infection, while others had not been previously reported. These data will be useful for selecting target genes for genetic engineering to control HLB.Gene expressionSweet orangeHuanglongbingPlant-pathogen interactioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/35651/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALve_Mafra_Valéria_etal_INI_2013.pdfve_Mafra_Valéria_etal_INI_2013.pdfapplication/pdf1026491https://www.arca.fiocruz.br/bitstream/icict/35651/2/ve_Mafra_Val%c3%a9ria_etal_INI_2013.pdf42f91dba11a9667ee6d575e69d2ebbeeMD52TEXTve_Mafra_Valéria_etal_INI_2013.pdf.txtve_Mafra_Valéria_etal_INI_2013.pdf.txtExtracted texttext/plain77810https://www.arca.fiocruz.br/bitstream/icict/35651/3/ve_Mafra_Val%c3%a9ria_etal_INI_2013.pdf.txt59ba7363a105634dee273d51da44e9ddMD53icict/356512019-09-19 02:01:10.382oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-09-19T05:01:10Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Candidatus Liberibacter americanus induces significant reprogramming of the transcriptome of the susceptible citrus genotype
title Candidatus Liberibacter americanus induces significant reprogramming of the transcriptome of the susceptible citrus genotype
spellingShingle Candidatus Liberibacter americanus induces significant reprogramming of the transcriptome of the susceptible citrus genotype
Mafra, Valéria
Gene expression
Sweet orange
Huanglongbing
Plant-pathogen interaction
title_short Candidatus Liberibacter americanus induces significant reprogramming of the transcriptome of the susceptible citrus genotype
title_full Candidatus Liberibacter americanus induces significant reprogramming of the transcriptome of the susceptible citrus genotype
title_fullStr Candidatus Liberibacter americanus induces significant reprogramming of the transcriptome of the susceptible citrus genotype
title_full_unstemmed Candidatus Liberibacter americanus induces significant reprogramming of the transcriptome of the susceptible citrus genotype
title_sort Candidatus Liberibacter americanus induces significant reprogramming of the transcriptome of the susceptible citrus genotype
author Mafra, Valéria
author_facet Mafra, Valéria
Martins, Polyana K.
Francisco, Carolina S.
Ribeiro-Alves, Marcelo
Freitas-Astúa, Juliana
Machado, Marcos A.
author_role author
author2 Martins, Polyana K.
Francisco, Carolina S.
Ribeiro-Alves, Marcelo
Freitas-Astúa, Juliana
Machado, Marcos A.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Mafra, Valéria
Martins, Polyana K.
Francisco, Carolina S.
Ribeiro-Alves, Marcelo
Freitas-Astúa, Juliana
Machado, Marcos A.
dc.subject.en.pt_BR.fl_str_mv Gene expression
Sweet orange
Huanglongbing
Plant-pathogen interaction
topic Gene expression
Sweet orange
Huanglongbing
Plant-pathogen interaction
description Centro de Citricultura Sylvio Moreira. Cordeirópolis, SP, Brasil / Instituto Agronômico de Campinas. Campinas, SP, Brasil / Universidade Estadual de Campinas. Campinas, SP, Brasil.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2019-09-18T12:29:54Z
dc.date.available.fl_str_mv 2019-09-18T12:29:54Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MAFRA, Valéria et al. Candidatus Liberibacter americanus induces significant reprogramming of the transcriptome of the susceptible citrus genotype. BMC Genomics, v. 14, p. 1-15, 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/35651
dc.identifier.issn.pt_BR.fl_str_mv 1471-2164
dc.identifier.doi.none.fl_str_mv 10.1186/1471-2164-14-247
dc.identifier.eissn.none.fl_str_mv 1471-2164
identifier_str_mv MAFRA, Valéria et al. Candidatus Liberibacter americanus induces significant reprogramming of the transcriptome of the susceptible citrus genotype. BMC Genomics, v. 14, p. 1-15, 2013.
1471-2164
10.1186/1471-2164-14-247
url https://www.arca.fiocruz.br/handle/icict/35651
dc.language.iso.fl_str_mv eng
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dc.publisher.none.fl_str_mv BMC
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dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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