Sample preparation for lab-on-a-chip systems in molecular diagnosis: a review

Cunha, Mylena Lemes; Silva, Stella Schuster da; Stracke, Mateus Cassaboni; Zanette, Dalila Luciola; Aoki, Mateus Nóbrega; Blanes, Lucas

Sample preparation for lab-on-a-chip systems in molecular diagnosis: a review

Detalhes bibliográficos
Autor(a) principal:	Cunha, Mylena Lemes
Data de Publicação:	2022
Outros Autores:	Silva, Stella Schuster da, Stracke, Mateus Cassaboni, Zanette, Dalila Luciola, Aoki, Mateus Nóbrega, Blanes, Lucas
Tipo de documento:	Artigo
Idioma:	por
Título da fonte:	Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo:	https://www.arca.fiocruz.br/handle/icict/51855
Resumo:	Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciencias e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil.

Metadados do item

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network_acronym_str	CRUZ
network_name_str	Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str	2135
spelling	Cunha, Mylena LemesSilva, Stella Schuster daStracke, Mateus CassaboniZanette, Dalila LuciolaAoki, Mateus NóbregaBlanes, Lucas2022-03-24T14:58:45Z2022-03-24T14:58:45Z2022CUNHA, Mylena Lemes et al. Sample Preparation for Lab-on-a-Chip Systems in Molecular Diagnosis: A Review. Anal. Chem., n. 94, p. 41−58, 2022.1520-6882https://www.arca.fiocruz.br/handle/icict/5185510.1021/acs.analchem.1c04460porAmerican Chemical SocietySample preparation for lab-on-a-chip systems in molecular diagnosis: a reviewinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciencias e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciencias e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciencias e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciencias e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciencias e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciencias e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Rapid and low-cost molecular analysis is especially required for early and specific diagnostics, quick decision-making, and sparing patients from unnecessary tests and hospitals from extra costs. One way to achieve this objective is through automated molecular diagnostic devices. Thus, sample-to-answer microfluidic devices are emerging with the promise of delivering a complete molecular diagnosis system that includes nucleic acid extraction, amplification, and detection steps in a single device. The biggest issue in such equipment is the extraction process, which is normally laborious and time-consuming but extremely important for sensitive and specific detection. Therefore, this Review focuses on automated or semiautomated extraction methodologies used in lab-on-a-chip devices. More than 15 different extraction methods developed over the past 10 years have been analyzed in terms of their advantages and disadvantages to improve extraction procedures in future studies. Herein, we are able to explain the high applicability of the extraction methodologies due to the large variety of samples in which different techniques were employed, showing that their applications are not limited to medical diagnosis. Moreover, we are able to conclude that further research in the field would be beneficial because the methodologies presented can be affordable, portable, time efficient, and easily manipulated, all of which are strong qualities for point-of-care technologies.Molecular Diagnostic TestingLab-On-A-Chip DevicesAnalytic Sample Preparation MethodsPoint-of-Care SystemsTécnicas de Diagnóstico MolecularDispositivos Laboratorio en un ChipMétodos Analíticos de la Preparación de la MuestraSistemas de Atención de PuntoTechniques de diagnostic moléculaireLaboratoires sur pucesMéthodes de préparation d'échantillons analytiquesSystèmes automatisés lit maladeTeste de Diagnóstico MolecularDispositivos Analíticos em MicrochipsMétodos Analíticos de Preparação de AmostrasSistemas Automatizados de Assistência Junto ao Leitoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv	Sample preparation for lab-on-a-chip systems in molecular diagnosis: a review
title	Sample preparation for lab-on-a-chip systems in molecular diagnosis: a review
spellingShingle	Sample preparation for lab-on-a-chip systems in molecular diagnosis: a review Cunha, Mylena Lemes Molecular Diagnostic Testing Lab-On-A-Chip Devices Analytic Sample Preparation Methods Point-of-Care Systems Técnicas de Diagnóstico Molecular Dispositivos Laboratorio en un Chip Métodos Analíticos de la Preparación de la Muestra Sistemas de Atención de Punto Techniques de diagnostic moléculaire Laboratoires sur puces Méthodes de préparation d'échantillons analytiques Systèmes automatisés lit malade Teste de Diagnóstico Molecular Dispositivos Analíticos em Microchips Métodos Analíticos de Preparação de Amostras Sistemas Automatizados de Assistência Junto ao Leito
title_short	Sample preparation for lab-on-a-chip systems in molecular diagnosis: a review
title_full	Sample preparation for lab-on-a-chip systems in molecular diagnosis: a review
title_fullStr	Sample preparation for lab-on-a-chip systems in molecular diagnosis: a review
title_full_unstemmed	Sample preparation for lab-on-a-chip systems in molecular diagnosis: a review
title_sort	Sample preparation for lab-on-a-chip systems in molecular diagnosis: a review
author	Cunha, Mylena Lemes
author_facet	Cunha, Mylena Lemes Silva, Stella Schuster da Stracke, Mateus Cassaboni Zanette, Dalila Luciola Aoki, Mateus Nóbrega Blanes, Lucas
author_role	author
author2	Silva, Stella Schuster da Stracke, Mateus Cassaboni Zanette, Dalila Luciola Aoki, Mateus Nóbrega Blanes, Lucas
author2_role	author author author author author
dc.contributor.author.fl_str_mv	Cunha, Mylena Lemes Silva, Stella Schuster da Stracke, Mateus Cassaboni Zanette, Dalila Luciola Aoki, Mateus Nóbrega Blanes, Lucas
dc.subject.en.pt_BR.fl_str_mv	Molecular Diagnostic Testing Lab-On-A-Chip Devices Analytic Sample Preparation Methods Point-of-Care Systems
topic	Molecular Diagnostic Testing Lab-On-A-Chip Devices Analytic Sample Preparation Methods Point-of-Care Systems Técnicas de Diagnóstico Molecular Dispositivos Laboratorio en un Chip Métodos Analíticos de la Preparación de la Muestra Sistemas de Atención de Punto Techniques de diagnostic moléculaire Laboratoires sur puces Méthodes de préparation d'échantillons analytiques Systèmes automatisés lit malade Teste de Diagnóstico Molecular Dispositivos Analíticos em Microchips Métodos Analíticos de Preparação de Amostras Sistemas Automatizados de Assistência Junto ao Leito
dc.subject.es.pt_BR.fl_str_mv	Técnicas de Diagnóstico Molecular Dispositivos Laboratorio en un Chip Métodos Analíticos de la Preparación de la Muestra Sistemas de Atención de Punto
dc.subject.fr.pt_BR.fl_str_mv	Techniques de diagnostic moléculaire Laboratoires sur puces Méthodes de préparation d'échantillons analytiques Systèmes automatisés lit malade
dc.subject.decs.pt_BR.fl_str_mv	Teste de Diagnóstico Molecular Dispositivos Analíticos em Microchips Métodos Analíticos de Preparação de Amostras Sistemas Automatizados de Assistência Junto ao Leito
description	Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciencias e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil.
publishDate	2022
dc.date.accessioned.fl_str_mv	2022-03-24T14:58:45Z
dc.date.available.fl_str_mv	2022-03-24T14:58:45Z
dc.date.issued.fl_str_mv	2022
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.citation.fl_str_mv	CUNHA, Mylena Lemes et al. Sample Preparation for Lab-on-a-Chip Systems in Molecular Diagnosis: A Review. Anal. Chem., n. 94, p. 41−58, 2022.
dc.identifier.uri.fl_str_mv	https://www.arca.fiocruz.br/handle/icict/51855
dc.identifier.issn.pt_BR.fl_str_mv	1520-6882
dc.identifier.doi.none.fl_str_mv	10.1021/acs.analchem.1c04460
identifier_str_mv	CUNHA, Mylena Lemes et al. Sample Preparation for Lab-on-a-Chip Systems in Molecular Diagnosis: A Review. Anal. Chem., n. 94, p. 41−58, 2022. 1520-6882 10.1021/acs.analchem.1c04460
url	https://www.arca.fiocruz.br/handle/icict/51855
dc.language.iso.fl_str_mv	por
language	por
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.publisher.none.fl_str_mv	American Chemical Society
publisher.none.fl_str_mv	American Chemical Society
dc.source.none.fl_str_mv	reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ
instname_str	Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str	FIOCRUZ
institution	FIOCRUZ
reponame_str	Repositório Institucional da FIOCRUZ (ARCA)
collection	Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv	https://www.arca.fiocruz.br/bitstream/icict/51855/1/license.txt https://www.arca.fiocruz.br/bitstream/icict/51855/2/acs.analchem.1c04460-OK.pdf
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bitstream.checksumAlgorithm.fl_str_mv	MD5 MD5
repository.name.fl_str_mv	Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv	repositorio.arca@fiocruz.br
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Sample preparation for lab-on-a-chip systems in molecular diagnosis: a review

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