Gene Expression Signatures in AML-12 Hepatocyte Cells upon Dengue virus Infection and Acetaminophen Treatment

Detalhes bibliográficos
Autor(a) principal: Ferreira, Jorge Gomes Goulart
Data de Publicação: 2020
Outros Autores: Gava, Sandra Grossi, Oliveira, Eneida Santos de, Batista, Izabella Cristina Andrade, Fernandes, Gabriel da Rocha, Mourão, Marina de Moraes, Silva, Carlos Eduardo Calzavara
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/58725
Resumo: Fundação Oswaldo Cruz. Instituto René Rachou. Imunologia Celular e Molecular Belo Horizonte, MG, Brazil
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spelling Ferreira, Jorge Gomes GoulartGava, Sandra GrossiOliveira, Eneida Santos deBatista, Izabella Cristina AndradeFernandes, Gabriel da RochaMourão, Marina de MoraesSilva, Carlos Eduardo Calzavara2023-05-25T16:53:48Z2023-05-25T16:53:48Z2020FERREIRA, Jorge Gomes Goulart et al. Gene Expression Signatures in AML-12 Hepatocyte Cells upon Dengue virus Infection and Acetaminophen Treatment. Viruses, v. 12, n. 11, 1284, 2020. doi: 10.3390/v121112841999-4915https://www.arca.fiocruz.br/handle/icict/5872510.3390/v12111284engMDPIGene Expression Signatures in AML-12 Hepatocyte Cells upon Dengue virus Infection and Acetaminophen Treatmentinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto René Rachou. Imunologia Celular e Molecular Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto René Rachou. Helmintologia e Malacologia Médica. Belo Horizonte, MG, BrazilPrefeitura de Belo Horizonte. Secretaria Municipal de Saúde. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto René Rachou. Imunologia Celular e Molecular Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto René Rachou. Informática de Biossistemas. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto René Rachou. Helmintologia e Malacologia Médica. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto René Rachou. Imunologia Celular e Molecular Belo Horizonte, MG, BrazilDengue is an acute viral disease caused by Dengue virus (DENV) and is considered to be the most common arbovirus worldwide. The clinical characteristics of dengue may vary from asymptomatic to severe complications and severe organ impairment, particularly affecting the liver. Dengue treatment is palliative with acetaminophen (APAP), usually known as Paracetamol, being the most used drug aiming to relieve the mild symptoms of dengue. APAP is a safe and effective drug but, like dengue, can trigger the development of liver disorders. Given this scenario, it is necessary to investigate the effects of combining these two factors on hepatocyte homeostasis. Therefore, this study aimed to evaluate the molecular changes in hepatocytes resulting from the association between DENV infection and treatment with sub-toxic APAP concentrations. Using an in vitro experimental model of DENV-2 infected hepatocytes (AML-12 cells) treated with APAP, we evaluated the influence of the virus and drug association on the transcriptome of these hepatocytes by RNA sequencing (RNAseq). The virus-drug association was able to induce changes in the gene expression profile of AML-12 cells and here we highlight and explore these changes and its putative influence on biological processes for cellular homeostasis.DengueDENVacetaminophenhepatocytestranscriptomeRNA sequencing (RNAseq)info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALGene Expression Signatures in AML-12 Hepatocyte Cells upon Dengue virus Infection and Acetaminophen Treatment.pdfGene Expression Signatures in AML-12 Hepatocyte Cells upon Dengue virus Infection and Acetaminophen Treatment.pdfapplication/pdf4774655https://www.arca.fiocruz.br/bitstream/icict/58725/2/Gene%20Expression%20Signatures%20in%20AML-12%20Hepatocyte%20Cells%20upon%20Dengue%20virus%20Infection%20and%20Acetaminophen%20Treatment.pdfbf75d2d18794da90b423711042673f6eMD52LICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/58725/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51icict/587252023-05-25 13:53:48.8oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-05-25T16:53:48Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en_US.fl_str_mv Gene Expression Signatures in AML-12 Hepatocyte Cells upon Dengue virus Infection and Acetaminophen Treatment
title Gene Expression Signatures in AML-12 Hepatocyte Cells upon Dengue virus Infection and Acetaminophen Treatment
spellingShingle Gene Expression Signatures in AML-12 Hepatocyte Cells upon Dengue virus Infection and Acetaminophen Treatment
Ferreira, Jorge Gomes Goulart
Dengue
DENV
acetaminophen
hepatocytes
transcriptome
RNA sequencing (RNAseq)
title_short Gene Expression Signatures in AML-12 Hepatocyte Cells upon Dengue virus Infection and Acetaminophen Treatment
title_full Gene Expression Signatures in AML-12 Hepatocyte Cells upon Dengue virus Infection and Acetaminophen Treatment
title_fullStr Gene Expression Signatures in AML-12 Hepatocyte Cells upon Dengue virus Infection and Acetaminophen Treatment
title_full_unstemmed Gene Expression Signatures in AML-12 Hepatocyte Cells upon Dengue virus Infection and Acetaminophen Treatment
title_sort Gene Expression Signatures in AML-12 Hepatocyte Cells upon Dengue virus Infection and Acetaminophen Treatment
author Ferreira, Jorge Gomes Goulart
author_facet Ferreira, Jorge Gomes Goulart
Gava, Sandra Grossi
Oliveira, Eneida Santos de
Batista, Izabella Cristina Andrade
Fernandes, Gabriel da Rocha
Mourão, Marina de Moraes
Silva, Carlos Eduardo Calzavara
author_role author
author2 Gava, Sandra Grossi
Oliveira, Eneida Santos de
Batista, Izabella Cristina Andrade
Fernandes, Gabriel da Rocha
Mourão, Marina de Moraes
Silva, Carlos Eduardo Calzavara
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Ferreira, Jorge Gomes Goulart
Gava, Sandra Grossi
Oliveira, Eneida Santos de
Batista, Izabella Cristina Andrade
Fernandes, Gabriel da Rocha
Mourão, Marina de Moraes
Silva, Carlos Eduardo Calzavara
dc.subject.en.en_US.fl_str_mv Dengue
DENV
acetaminophen
hepatocytes
transcriptome
RNA sequencing (RNAseq)
topic Dengue
DENV
acetaminophen
hepatocytes
transcriptome
RNA sequencing (RNAseq)
description Fundação Oswaldo Cruz. Instituto René Rachou. Imunologia Celular e Molecular Belo Horizonte, MG, Brazil
publishDate 2020
dc.date.issued.fl_str_mv 2020
dc.date.accessioned.fl_str_mv 2023-05-25T16:53:48Z
dc.date.available.fl_str_mv 2023-05-25T16:53:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FERREIRA, Jorge Gomes Goulart et al. Gene Expression Signatures in AML-12 Hepatocyte Cells upon Dengue virus Infection and Acetaminophen Treatment. Viruses, v. 12, n. 11, 1284, 2020. doi: 10.3390/v12111284
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/58725
dc.identifier.issn.en_US.fl_str_mv 1999-4915
dc.identifier.doi.none.fl_str_mv 10.3390/v12111284
identifier_str_mv FERREIRA, Jorge Gomes Goulart et al. Gene Expression Signatures in AML-12 Hepatocyte Cells upon Dengue virus Infection and Acetaminophen Treatment. Viruses, v. 12, n. 11, 1284, 2020. doi: 10.3390/v12111284
1999-4915
10.3390/v12111284
url https://www.arca.fiocruz.br/handle/icict/58725
dc.language.iso.fl_str_mv eng
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
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