Insulin-like growth factor-I serum levels and their biological effects on Leishmania isolates from different clinical forms of American tegumentary leishmaniasis

Detalhes bibliográficos
Autor(a) principal: Souza, Luana Dias de
Data de Publicação: 2016
Outros Autores: Vendrame, Célia Maria Vieira, Jesus, Amélia Ribeiro de, Carvalho, Márcia Dias Teixeira, Schriefer, Albert, Guimarães, Luiz Henrique, Carvalho Filho, Edgar Marcelino, Goto, Hiro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/18349
Resumo: Carvalho, E. M. Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.
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spelling Souza, Luana Dias deVendrame, Célia Maria VieiraJesus, Amélia Ribeiro deCarvalho, Márcia Dias TeixeiraSchriefer, AlbertGuimarães, Luiz HenriqueCarvalho Filho, Edgar MarcelinoGoto, Hiro2017-04-10T18:25:55Z2017-04-10T18:25:55Z2016SOUZA, L. D. et al. Insulin-like growth factor-I serum levels and their biological effects on Leishmania isolates from different clinical forms of American tegumentary leishmaniasis. Parasites & Vectors, v. 9, p. 335, 2016.1756-3305https://www.arca.fiocruz.br/handle/icict/1834910.1186/s13071-016-1619-xCarvalho, E. M. Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.Fundação de Amparo à Pesquisa do Estado de São Paulo (research fellowship 2008/2209-6 to LDS), Fundação de Amparo à Pesquisa do Estado da Bahia (research fellowship 1780–2007 to CMVV), Conselho Nacional de Pesquisa (fellowship to EMC, ARJ and HG, and grant 484499/2006) and the Soroepidemiology and Immunobiology Laboratory LIM/38 (HC-FMUSP).Universidade de São Paulo. Instituto de Medicina Tropical. Laboratório de Soroepidemiologia e Imunobiologia. São Paulo, SP, BrasilUniversidade de São Paulo. Instituto de Medicina Tropical. Laboratório de Soroepidemiologia e Imunobiologia. São Paulo, SP, Brasil / Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilUniversidade Federal de Sergipe. Laboratório de Biologia Molecular. Departamento de Medicina Interna e Patologia. Aracaju, SE, BrasilUniversidade de São Paulo. Instituto de Medicina Tropical. Laboratório de Soroepidemiologia e Imunobiologia. São Paulo, SP, BrasilUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilUniversidade de São Paulo. Instituto de Medicina Tropical. Laboratório de Soroepidemiologia e Imunobiologia. São Paulo, SP, Brasil /Universidade de São Paulo. Faculdade de Medicina. Departamento de Medicina Preventiva. São Paulo, SP, BrasilAmerican tegumentary leishmaniasis (ATL) in Brazil is mostly caused by Leishmania (Viannia) braziliensis, with known forms of the disease being cutaneous (CL), mucosal (ML) and disseminated (DL) leishmaniasis. The development of the lesion in ATL is related both to the persistence of the Leishmania in the skin and to the parasite-triggered immune and inflammatory responses that ensue lesions. In this context one factor with expected role in the pathogenesis is insulin-like growth factor (IGF)-I with known effects on parasite growth and healing and inflammatory processes. In the present study, we addressed the effect of IGF-I on intracellular amastigote isolates from CL, ML and DL patients within human macrophage and we evaluated the IGF-I and IGF-binding protein-3 (IGFBP3) serum levels in patients presenting different clinical forms and controls from the endemic area. Methods: We evaluated biological variability in the responses of intracellular amastigotes of Leishmania isolates derived from CL, ML, and DL patients from an area for ATL in response to IGF-I. Intracellular amastigote growth was evaluated using the human macrophage cell line THP-1. Arginase activity in infected cells was evaluated quantifying the generated urea concentration. Serum samples from patients and controls were assayed using chemiluminescent immunometric assay to determine IGF-I and IGFBP3 levels. Results: We observed an increase in intracellular parasitism upon IGF-I stimulus in 62.5 % of isolates from CL, in 85.7 % from ML and only 42.8 % from DL cases. In DL, the basal arginase activity was lower than that of CL. We then evaluated the IGF-I and IGFBP3 serum levels in patients, and we observed significantly lower levels in ML and DL than in CL and control samples. Conclusions: The data suggest that IGF-I is modulated distinctly in different clinical forms of tegumentary leishmaniasis. IGF-I seemingly exerts effect on parasite growth likely contributing to its persistence in the skin in earlier phase. In addition the decreased IGF-I serum levels may affect the modulation of inflammation and lesion healing in chronic phase. In view of potential role of IGF-I in the pathogenesis of ATL we can speculate on therapeutic procedures taking into account the local IGF-I level.engBioMed CentralLeishmania braziliensisLeishmaniose tegumentarTHP-1Nível de IGF-ILeishmania braziliensisTegumentary leishmaniasisTHP-1IGF-I levelInsulin-like growth factor-I serum levels and their biological effects on Leishmania isolates from different clinical forms of American tegumentary leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/18349/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSouza LD Insulin-like....pdfSouza LD 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dc.title.pt_BR.fl_str_mv Insulin-like growth factor-I serum levels and their biological effects on Leishmania isolates from different clinical forms of American tegumentary leishmaniasis
title Insulin-like growth factor-I serum levels and their biological effects on Leishmania isolates from different clinical forms of American tegumentary leishmaniasis
spellingShingle Insulin-like growth factor-I serum levels and their biological effects on Leishmania isolates from different clinical forms of American tegumentary leishmaniasis
Souza, Luana Dias de
Leishmania braziliensis
Leishmaniose tegumentar
THP-1
Nível de IGF-I
Leishmania braziliensis
Tegumentary leishmaniasis
THP-1
IGF-I level
title_short Insulin-like growth factor-I serum levels and their biological effects on Leishmania isolates from different clinical forms of American tegumentary leishmaniasis
title_full Insulin-like growth factor-I serum levels and their biological effects on Leishmania isolates from different clinical forms of American tegumentary leishmaniasis
title_fullStr Insulin-like growth factor-I serum levels and their biological effects on Leishmania isolates from different clinical forms of American tegumentary leishmaniasis
title_full_unstemmed Insulin-like growth factor-I serum levels and their biological effects on Leishmania isolates from different clinical forms of American tegumentary leishmaniasis
title_sort Insulin-like growth factor-I serum levels and their biological effects on Leishmania isolates from different clinical forms of American tegumentary leishmaniasis
author Souza, Luana Dias de
author_facet Souza, Luana Dias de
Vendrame, Célia Maria Vieira
Jesus, Amélia Ribeiro de
Carvalho, Márcia Dias Teixeira
Schriefer, Albert
Guimarães, Luiz Henrique
Carvalho Filho, Edgar Marcelino
Goto, Hiro
author_role author
author2 Vendrame, Célia Maria Vieira
Jesus, Amélia Ribeiro de
Carvalho, Márcia Dias Teixeira
Schriefer, Albert
Guimarães, Luiz Henrique
Carvalho Filho, Edgar Marcelino
Goto, Hiro
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Souza, Luana Dias de
Vendrame, Célia Maria Vieira
Jesus, Amélia Ribeiro de
Carvalho, Márcia Dias Teixeira
Schriefer, Albert
Guimarães, Luiz Henrique
Carvalho Filho, Edgar Marcelino
Goto, Hiro
dc.subject.other.pt_BR.fl_str_mv Leishmania braziliensis
Leishmaniose tegumentar
THP-1
Nível de IGF-I
topic Leishmania braziliensis
Leishmaniose tegumentar
THP-1
Nível de IGF-I
Leishmania braziliensis
Tegumentary leishmaniasis
THP-1
IGF-I level
dc.subject.en.pt_BR.fl_str_mv Leishmania braziliensis
Tegumentary leishmaniasis
THP-1
IGF-I level
description Carvalho, E. M. Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2017-04-10T18:25:55Z
dc.date.available.fl_str_mv 2017-04-10T18:25:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SOUZA, L. D. et al. Insulin-like growth factor-I serum levels and their biological effects on Leishmania isolates from different clinical forms of American tegumentary leishmaniasis. Parasites & Vectors, v. 9, p. 335, 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/18349
dc.identifier.issn.pt_BR.fl_str_mv 1756-3305
dc.identifier.doi.none.fl_str_mv 10.1186/s13071-016-1619-x
identifier_str_mv SOUZA, L. D. et al. Insulin-like growth factor-I serum levels and their biological effects on Leishmania isolates from different clinical forms of American tegumentary leishmaniasis. Parasites & Vectors, v. 9, p. 335, 2016.
1756-3305
10.1186/s13071-016-1619-x
url https://www.arca.fiocruz.br/handle/icict/18349
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/18349/2/Souza%20LD%20Insulin-like....pdf
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