Drk1, a dimorphism histidine kinase, contributes to morphology, virulence, and stress adaptation in Paracoccidioides brasiliensis

Detalhes bibliográficos
Autor(a) principal: Marcos, Caroline Maria
Data de Publicação: 2021
Outros Autores: Oliveira, Haroldo Cesar de, Assato, Patrícia Akemi, Castelli, Rafael Fernando, Fusco-Almeida, Ana Marisa, Mendes-Giannini, Maria José Soares
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/50063
Resumo: Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas, Araraquara, SP, Brasil.
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spelling Marcos, Caroline MariaOliveira, Haroldo Cesar deAssato, Patrícia AkemiCastelli, Rafael FernandoFusco-Almeida, Ana MarisaMendes-Giannini, Maria José Soares2021-11-25T16:47:59Z2021-11-25T16:47:59Z2021MARCOS, Caroline Maria et al. Drk1, a dimorphism histidine kinase, contributes to morphology, virulence, and stress adaptation in Paracoccidioides brasiliensis. J. Fungi, V.7, n. 852, p. 1–22, 2021.2309-608Xhttps://www.arca.fiocruz.br/handle/icict/5006310.3390/jof7100852porMDPIDrk1Histidine KinaseParacoccidioides brasiliensisBroadly Neutralizing AntibodiesRNA SilencingCell WallVirulenceHistidina QuinasaParacoccidioidesAnticuerpos ampliamente neutralizantesInterferencia de ARNPared CelularVirulenciaHistidine kinaseParacoccidioidesInterférence par ARNParoi cellulaireHistidina QuinaseParacoccidioidesAnticorpos Amplamente NeutralizantesInterferência de RNAParede CelularVirulênciaDrk1, a dimorphism histidine kinase, contributes to morphology, virulence, and stress adaptation in Paracoccidioides brasiliensisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Estadual Paulista. Faculdade de Ciências Farmacêuticas, Araraquara, SP, Brasil.Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas, Araraquara, SP, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas, Araraquara, SP, Brasil. / Universidade Estadual Paulista. Faculdade de Ciências Agronômicas. Laboratório Central Multiusuários. Botucatu, SP, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Programa de Pós-Graduação em Biologia Parasitária. Rio de Janeiro, RJ, Brasil.Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas, Araraquara, SP, Brasil.Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas, Araraquara, SP, Brasil.P. brasiliensis is a thermally dimorphic fungus belonging to Paracoccidioides complex, causative of a systemic, endemic mycosis limited to Latin American countries. Signal transduction pathways related to important aspects as surviving, proliferation according to the biological niches are linked to the fungal pathogenicity in many species, but its elucidation in P. brasiliensis remains poorly explored. As Drk1, a hybrid histidine kinase, plays regulators functions in other dimorphic fungi species, mainly in dimorphism and virulence, here we investigated its importance in P. brasilensis. We, therefore generated the respective recombinant protein, anti-PbDrk1 polyclonal antibody and a silenced strain. The Drk1 protein shows a random distribution including cell wall location that change its pattern during osmotic stress condition; moreover the P. brasiliensis treatment with anti-PbDrk1 antibody, which does not modify the fungus’s viability, resulted in decreased virulence in G. mellonella model and reduced interaction with pneumocytes. Down-regulating PbDRK1 yielded phenotypic alterations such as yeast cells with more elongated morphology, virulence attenuation in G. mellonella infection model, lower amount of chitin content, increased resistance to osmotic and cell wall stresses, and also caspofungin, and finally increased sensitivity to itraconazole. These observations highlight the importance of PbDrk1 to P. brasiliensis virulence, stress adaptation, morphology, and cell wall organization, and therefore it an interesting target that could help develop new antifungalsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/50063/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALjof-07-00852OK.pdfjof-07-00852OK.pdfapplication/pdf4430269https://www.arca.fiocruz.br/bitstream/icict/50063/2/jof-07-00852OK.pdfad8eb3f75ac0ad148941df7a9bc93de7MD52icict/500632021-11-25 13:47:59.691oai:www.arca.fiocruz.br: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ório 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dc.title.pt_BR.fl_str_mv Drk1, a dimorphism histidine kinase, contributes to morphology, virulence, and stress adaptation in Paracoccidioides brasiliensis
title Drk1, a dimorphism histidine kinase, contributes to morphology, virulence, and stress adaptation in Paracoccidioides brasiliensis
spellingShingle Drk1, a dimorphism histidine kinase, contributes to morphology, virulence, and stress adaptation in Paracoccidioides brasiliensis
Marcos, Caroline Maria
Drk1
Histidine Kinase
Paracoccidioides brasiliensis
Broadly Neutralizing Antibodies
RNA Silencing
Cell Wall
Virulence
Histidina Quinasa
Paracoccidioides
Anticuerpos ampliamente neutralizantes
Interferencia de ARN
Pared Celular
Virulencia
Histidine kinase
Paracoccidioides
Interférence par ARN
Paroi cellulaire
Histidina Quinase
Paracoccidioides
Anticorpos Amplamente Neutralizantes
Interferência de RNA
Parede Celular
Virulência
title_short Drk1, a dimorphism histidine kinase, contributes to morphology, virulence, and stress adaptation in Paracoccidioides brasiliensis
title_full Drk1, a dimorphism histidine kinase, contributes to morphology, virulence, and stress adaptation in Paracoccidioides brasiliensis
title_fullStr Drk1, a dimorphism histidine kinase, contributes to morphology, virulence, and stress adaptation in Paracoccidioides brasiliensis
title_full_unstemmed Drk1, a dimorphism histidine kinase, contributes to morphology, virulence, and stress adaptation in Paracoccidioides brasiliensis
title_sort Drk1, a dimorphism histidine kinase, contributes to morphology, virulence, and stress adaptation in Paracoccidioides brasiliensis
author Marcos, Caroline Maria
author_facet Marcos, Caroline Maria
Oliveira, Haroldo Cesar de
Assato, Patrícia Akemi
Castelli, Rafael Fernando
Fusco-Almeida, Ana Marisa
Mendes-Giannini, Maria José Soares
author_role author
author2 Oliveira, Haroldo Cesar de
Assato, Patrícia Akemi
Castelli, Rafael Fernando
Fusco-Almeida, Ana Marisa
Mendes-Giannini, Maria José Soares
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Marcos, Caroline Maria
Oliveira, Haroldo Cesar de
Assato, Patrícia Akemi
Castelli, Rafael Fernando
Fusco-Almeida, Ana Marisa
Mendes-Giannini, Maria José Soares
dc.subject.other.pt_BR.fl_str_mv Drk1
topic Drk1
Histidine Kinase
Paracoccidioides brasiliensis
Broadly Neutralizing Antibodies
RNA Silencing
Cell Wall
Virulence
Histidina Quinasa
Paracoccidioides
Anticuerpos ampliamente neutralizantes
Interferencia de ARN
Pared Celular
Virulencia
Histidine kinase
Paracoccidioides
Interférence par ARN
Paroi cellulaire
Histidina Quinase
Paracoccidioides
Anticorpos Amplamente Neutralizantes
Interferência de RNA
Parede Celular
Virulência
dc.subject.en.pt_BR.fl_str_mv Histidine Kinase
Paracoccidioides brasiliensis
Broadly Neutralizing Antibodies
RNA Silencing
Cell Wall
Virulence
dc.subject.es.pt_BR.fl_str_mv Histidina Quinasa
Paracoccidioides
Anticuerpos ampliamente neutralizantes
Interferencia de ARN
Pared Celular
Virulencia
dc.subject.fr.pt_BR.fl_str_mv Histidine kinase
Paracoccidioides
Interférence par ARN
Paroi cellulaire
dc.subject.decs.pt_BR.fl_str_mv Histidina Quinase
Paracoccidioides
Anticorpos Amplamente Neutralizantes
Interferência de RNA
Parede Celular
Virulência
description Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas, Araraquara, SP, Brasil.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-11-25T16:47:59Z
dc.date.available.fl_str_mv 2021-11-25T16:47:59Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MARCOS, Caroline Maria et al. Drk1, a dimorphism histidine kinase, contributes to morphology, virulence, and stress adaptation in Paracoccidioides brasiliensis. J. Fungi, V.7, n. 852, p. 1–22, 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/50063
dc.identifier.issn.pt_BR.fl_str_mv 2309-608X
dc.identifier.doi.none.fl_str_mv 10.3390/jof7100852
identifier_str_mv MARCOS, Caroline Maria et al. Drk1, a dimorphism histidine kinase, contributes to morphology, virulence, and stress adaptation in Paracoccidioides brasiliensis. J. Fungi, V.7, n. 852, p. 1–22, 2021.
2309-608X
10.3390/jof7100852
url https://www.arca.fiocruz.br/handle/icict/50063
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/50063/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/50063/2/jof-07-00852OK.pdf
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repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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