Drk1, a dimorphism histidine kinase, contributes to morphology, virulence, and stress adaptation in Paracoccidioides brasiliensis
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/50063 |
Resumo: | Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas, Araraquara, SP, Brasil. |
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Marcos, Caroline MariaOliveira, Haroldo Cesar deAssato, Patrícia AkemiCastelli, Rafael FernandoFusco-Almeida, Ana MarisaMendes-Giannini, Maria José Soares2021-11-25T16:47:59Z2021-11-25T16:47:59Z2021MARCOS, Caroline Maria et al. Drk1, a dimorphism histidine kinase, contributes to morphology, virulence, and stress adaptation in Paracoccidioides brasiliensis. J. Fungi, V.7, n. 852, p. 1–22, 2021.2309-608Xhttps://www.arca.fiocruz.br/handle/icict/5006310.3390/jof7100852porMDPIDrk1Histidine KinaseParacoccidioides brasiliensisBroadly Neutralizing AntibodiesRNA SilencingCell WallVirulenceHistidina QuinasaParacoccidioidesAnticuerpos ampliamente neutralizantesInterferencia de ARNPared CelularVirulenciaHistidine kinaseParacoccidioidesInterférence par ARNParoi cellulaireHistidina QuinaseParacoccidioidesAnticorpos Amplamente NeutralizantesInterferência de RNAParede CelularVirulênciaDrk1, a dimorphism histidine kinase, contributes to morphology, virulence, and stress adaptation in Paracoccidioides brasiliensisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Estadual Paulista. Faculdade de Ciências Farmacêuticas, Araraquara, SP, Brasil.Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas, Araraquara, SP, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas, Araraquara, SP, Brasil. / Universidade Estadual Paulista. Faculdade de Ciências Agronômicas. Laboratório Central Multiusuários. Botucatu, SP, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Programa de Pós-Graduação em Biologia Parasitária. Rio de Janeiro, RJ, Brasil.Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas, Araraquara, SP, Brasil.Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas, Araraquara, SP, Brasil.P. brasiliensis is a thermally dimorphic fungus belonging to Paracoccidioides complex, causative of a systemic, endemic mycosis limited to Latin American countries. Signal transduction pathways related to important aspects as surviving, proliferation according to the biological niches are linked to the fungal pathogenicity in many species, but its elucidation in P. brasiliensis remains poorly explored. As Drk1, a hybrid histidine kinase, plays regulators functions in other dimorphic fungi species, mainly in dimorphism and virulence, here we investigated its importance in P. brasilensis. We, therefore generated the respective recombinant protein, anti-PbDrk1 polyclonal antibody and a silenced strain. The Drk1 protein shows a random distribution including cell wall location that change its pattern during osmotic stress condition; moreover the P. brasiliensis treatment with anti-PbDrk1 antibody, which does not modify the fungus’s viability, resulted in decreased virulence in G. mellonella model and reduced interaction with pneumocytes. Down-regulating PbDRK1 yielded phenotypic alterations such as yeast cells with more elongated morphology, virulence attenuation in G. mellonella infection model, lower amount of chitin content, increased resistance to osmotic and cell wall stresses, and also caspofungin, and finally increased sensitivity to itraconazole. These observations highlight the importance of PbDrk1 to P. brasiliensis virulence, stress adaptation, morphology, and cell wall organization, and therefore it an interesting target that could help develop new antifungalsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/50063/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALjof-07-00852OK.pdfjof-07-00852OK.pdfapplication/pdf4430269https://www.arca.fiocruz.br/bitstream/icict/50063/2/jof-07-00852OK.pdfad8eb3f75ac0ad148941df7a9bc93de7MD52icict/500632021-11-25 13:47:59.691oai:www.arca.fiocruz.br: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ório 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dc.title.pt_BR.fl_str_mv |
Drk1, a dimorphism histidine kinase, contributes to morphology, virulence, and stress adaptation in Paracoccidioides brasiliensis |
title |
Drk1, a dimorphism histidine kinase, contributes to morphology, virulence, and stress adaptation in Paracoccidioides brasiliensis |
spellingShingle |
Drk1, a dimorphism histidine kinase, contributes to morphology, virulence, and stress adaptation in Paracoccidioides brasiliensis Marcos, Caroline Maria Drk1 Histidine Kinase Paracoccidioides brasiliensis Broadly Neutralizing Antibodies RNA Silencing Cell Wall Virulence Histidina Quinasa Paracoccidioides Anticuerpos ampliamente neutralizantes Interferencia de ARN Pared Celular Virulencia Histidine kinase Paracoccidioides Interférence par ARN Paroi cellulaire Histidina Quinase Paracoccidioides Anticorpos Amplamente Neutralizantes Interferência de RNA Parede Celular Virulência |
title_short |
Drk1, a dimorphism histidine kinase, contributes to morphology, virulence, and stress adaptation in Paracoccidioides brasiliensis |
title_full |
Drk1, a dimorphism histidine kinase, contributes to morphology, virulence, and stress adaptation in Paracoccidioides brasiliensis |
title_fullStr |
Drk1, a dimorphism histidine kinase, contributes to morphology, virulence, and stress adaptation in Paracoccidioides brasiliensis |
title_full_unstemmed |
Drk1, a dimorphism histidine kinase, contributes to morphology, virulence, and stress adaptation in Paracoccidioides brasiliensis |
title_sort |
Drk1, a dimorphism histidine kinase, contributes to morphology, virulence, and stress adaptation in Paracoccidioides brasiliensis |
author |
Marcos, Caroline Maria |
author_facet |
Marcos, Caroline Maria Oliveira, Haroldo Cesar de Assato, Patrícia Akemi Castelli, Rafael Fernando Fusco-Almeida, Ana Marisa Mendes-Giannini, Maria José Soares |
author_role |
author |
author2 |
Oliveira, Haroldo Cesar de Assato, Patrícia Akemi Castelli, Rafael Fernando Fusco-Almeida, Ana Marisa Mendes-Giannini, Maria José Soares |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Marcos, Caroline Maria Oliveira, Haroldo Cesar de Assato, Patrícia Akemi Castelli, Rafael Fernando Fusco-Almeida, Ana Marisa Mendes-Giannini, Maria José Soares |
dc.subject.other.pt_BR.fl_str_mv |
Drk1 |
topic |
Drk1 Histidine Kinase Paracoccidioides brasiliensis Broadly Neutralizing Antibodies RNA Silencing Cell Wall Virulence Histidina Quinasa Paracoccidioides Anticuerpos ampliamente neutralizantes Interferencia de ARN Pared Celular Virulencia Histidine kinase Paracoccidioides Interférence par ARN Paroi cellulaire Histidina Quinase Paracoccidioides Anticorpos Amplamente Neutralizantes Interferência de RNA Parede Celular Virulência |
dc.subject.en.pt_BR.fl_str_mv |
Histidine Kinase Paracoccidioides brasiliensis Broadly Neutralizing Antibodies RNA Silencing Cell Wall Virulence |
dc.subject.es.pt_BR.fl_str_mv |
Histidina Quinasa Paracoccidioides Anticuerpos ampliamente neutralizantes Interferencia de ARN Pared Celular Virulencia |
dc.subject.fr.pt_BR.fl_str_mv |
Histidine kinase Paracoccidioides Interférence par ARN Paroi cellulaire |
dc.subject.decs.pt_BR.fl_str_mv |
Histidina Quinase Paracoccidioides Anticorpos Amplamente Neutralizantes Interferência de RNA Parede Celular Virulência |
description |
Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas, Araraquara, SP, Brasil. |
publishDate |
2021 |
dc.date.accessioned.fl_str_mv |
2021-11-25T16:47:59Z |
dc.date.available.fl_str_mv |
2021-11-25T16:47:59Z |
dc.date.issued.fl_str_mv |
2021 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
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article |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
MARCOS, Caroline Maria et al. Drk1, a dimorphism histidine kinase, contributes to morphology, virulence, and stress adaptation in Paracoccidioides brasiliensis. J. Fungi, V.7, n. 852, p. 1–22, 2021. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/50063 |
dc.identifier.issn.pt_BR.fl_str_mv |
2309-608X |
dc.identifier.doi.none.fl_str_mv |
10.3390/jof7100852 |
identifier_str_mv |
MARCOS, Caroline Maria et al. Drk1, a dimorphism histidine kinase, contributes to morphology, virulence, and stress adaptation in Paracoccidioides brasiliensis. J. Fungi, V.7, n. 852, p. 1–22, 2021. 2309-608X 10.3390/jof7100852 |
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https://www.arca.fiocruz.br/handle/icict/50063 |
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