Gene expression network analyses during infection with virulent and avirulent Trypanosoma cruzi strains unveil a role for fibroblasts in neutrophil recruitment and activation
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/43693 |
Resumo: | Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil |
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Oliveira, Antonio Edson R.Pereira, Milton C. A.Belew, Ashton T.Ferreira, Ludmila R. P.Pereira, Larissa Marina NogueiraNeves, Eula G. A.Nunes, Maria do Carmo P.Burleigh, Barbara A.Dutra, Walderez OrnelasEl-Sayed, Najib M.Gazzinelli, Ricardo TostesTeixeira, Santuza M. R.2020-09-29T18:46:31Z2020-09-29T18:46:31Z2020OLIVEIRA, Antonio Edson R et al. Gene expression network analyses during infection with virulent and avirulentTrypanosoma cruzi strains unveil a role for fibroblasts in neutrophil recruitment and activation. PLoSPathog, v. 16, n. 8, e1008781, 2020. .https://doi.org/10.1371/journal.ppat.10087811553-7374https://www.arca.fiocruz.br/handle/icict/4369310.1371/journal.ppat.1008781engPublic Library of ScienceGene expression network analyses during infection with virulent and avirulent Trypanosoma cruzi strains unveil a role for fibroblasts in neutrophil recruitment and activationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, BrazilDepartment of Cell Biology and Molecular Genetics and Center for Bioinformatics and Computational Biology. University of Maryland.College Park, Maryland, United States of AmericaUniversidade Federal de Minas Gerais. Departamento de Morfologia. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Departamento de Morfologia. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Faculdade de Medicina. Departamento de Clínica Médica. Belo Horizonte, MG, BrazilDepartment of Immunology and Infectious Diseases. Harvard T. H. Chan School of Public Health. Boston, MA, United States of AmericaUniversidade Federal de Minas Gerais. Departamento de Morfologia. Belo Horizonte, MG, BrazilDepartment of Cell Biology and Molecular Genetics and Center for Bioinformatics and Computational Biology., University of Maryland.College Park, Maryland, United States of AmericaUniversidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil/Fundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrazilChagas disease is caused by Trypanosoma cruzi, a protozoan parasite that has a heterogeneous population composed of a pool of strains with distinct characteristics, including variable levels of virulence. In previous work, transcriptome analyses of parasite genes after infection of human foreskin fibroblasts (HFF) with virulent (CL Brener) and non-virulent (CL-14) clones derived from the CL strain, revealed a reduced expression of genes encoding parasite surface proteins in CL-14 compared to CL Brener during the final steps of the intracellular differentiation from amastigotes to trypomastigotes. Here we analyzed changes in the expression of host genes during in vitro infection of HFF cells with the CL Brener and CL-14 strains by analyzing total RNA extracted from cells at 60 and 96 hours post-infection (hpi) with each strain, as well as from uninfected cells. Similar transcriptome profiles were observed at 60 hpi with both strains compared to uninfected samples. However, at 96 hpi, significant differences in the number and expression levels of several genes, particularly those involved with immune response and cytoskeleton organization, were observed. Further analyses confirmed the difference in the chemokine/cytokine signaling involved with the recruitment and activation of immune cells such as neutrophils upon T. cruzi infection. These findings suggest that infection with the virulent CL Brener strain induces a more robust inflammatory response when compared with the non-virulent CL-14 strain. Importantly, the RNA-Seq data also exposed an unexplored role of fibroblasts as sentinel cells that may act by recruiting neutrophils to the initial site of infection. This role for fibroblasts in the regulation of the inflammatory response during infection by T. cruzi was corroborated by measurements of levels of different chemokines/cytokines during in vitro infection and in plasma from Chagas disease patients as well as by neutrophil activation and migration assays.Parasitic diseasesTrypanosoma cruziNeutrophilsFibroblastsCytokinesImmune responseGene expressionTranscriptome analysisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/43693/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALGene expression network analyses during .pdfGene expression network analyses during .pdfapplication/pdf14161595https://www.arca.fiocruz.br/bitstream/icict/43693/2/Gene%20expression%20network%20analyses%20during%20.pdf299ddeb923bbaa10c53dbcdd0d6540cdMD52TEXTGene expression network analyses during .pdf.txtGene expression network analyses during .pdf.txtExtracted texttext/plain27https://www.arca.fiocruz.br/bitstream/icict/43693/3/Gene%20expression%20network%20analyses%20during%20.pdf.txt9e469f9f01cf48ac7499fddb60354d66MD53icict/436932020-09-30 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| dc.title.pt_BR.fl_str_mv |
Gene expression network analyses during infection with virulent and avirulent Trypanosoma cruzi strains unveil a role for fibroblasts in neutrophil recruitment and activation |
| title |
Gene expression network analyses during infection with virulent and avirulent Trypanosoma cruzi strains unveil a role for fibroblasts in neutrophil recruitment and activation |
| spellingShingle |
Gene expression network analyses during infection with virulent and avirulent Trypanosoma cruzi strains unveil a role for fibroblasts in neutrophil recruitment and activation Oliveira, Antonio Edson R. Parasitic diseases Trypanosoma cruzi Neutrophils Fibroblasts Cytokines Immune response Gene expression Transcriptome analysis |
| title_short |
Gene expression network analyses during infection with virulent and avirulent Trypanosoma cruzi strains unveil a role for fibroblasts in neutrophil recruitment and activation |
| title_full |
Gene expression network analyses during infection with virulent and avirulent Trypanosoma cruzi strains unveil a role for fibroblasts in neutrophil recruitment and activation |
| title_fullStr |
Gene expression network analyses during infection with virulent and avirulent Trypanosoma cruzi strains unveil a role for fibroblasts in neutrophil recruitment and activation |
| title_full_unstemmed |
Gene expression network analyses during infection with virulent and avirulent Trypanosoma cruzi strains unveil a role for fibroblasts in neutrophil recruitment and activation |
| title_sort |
Gene expression network analyses during infection with virulent and avirulent Trypanosoma cruzi strains unveil a role for fibroblasts in neutrophil recruitment and activation |
| author |
Oliveira, Antonio Edson R. |
| author_facet |
Oliveira, Antonio Edson R. Pereira, Milton C. A. Belew, Ashton T. Ferreira, Ludmila R. P. Pereira, Larissa Marina Nogueira Neves, Eula G. A. Nunes, Maria do Carmo P. Burleigh, Barbara A. Dutra, Walderez Ornelas El-Sayed, Najib M. Gazzinelli, Ricardo Tostes Teixeira, Santuza M. R. |
| author_role |
author |
| author2 |
Pereira, Milton C. A. Belew, Ashton T. Ferreira, Ludmila R. P. Pereira, Larissa Marina Nogueira Neves, Eula G. A. Nunes, Maria do Carmo P. Burleigh, Barbara A. Dutra, Walderez Ornelas El-Sayed, Najib M. Gazzinelli, Ricardo Tostes Teixeira, Santuza M. R. |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Oliveira, Antonio Edson R. Pereira, Milton C. A. Belew, Ashton T. Ferreira, Ludmila R. P. Pereira, Larissa Marina Nogueira Neves, Eula G. A. Nunes, Maria do Carmo P. Burleigh, Barbara A. Dutra, Walderez Ornelas El-Sayed, Najib M. Gazzinelli, Ricardo Tostes Teixeira, Santuza M. R. |
| dc.subject.en.pt_BR.fl_str_mv |
Parasitic diseases Trypanosoma cruzi Neutrophils Fibroblasts Cytokines Immune response Gene expression Transcriptome analysis |
| topic |
Parasitic diseases Trypanosoma cruzi Neutrophils Fibroblasts Cytokines Immune response Gene expression Transcriptome analysis |
| description |
Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil |
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2020 |
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2020-09-29T18:46:31Z |
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2020-09-29T18:46:31Z |
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2020 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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OLIVEIRA, Antonio Edson R et al. Gene expression network analyses during infection with virulent and avirulentTrypanosoma cruzi strains unveil a role for fibroblasts in neutrophil recruitment and activation. PLoSPathog, v. 16, n. 8, e1008781, 2020. .https://doi.org/10.1371/journal.ppat.1008781 |
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https://www.arca.fiocruz.br/handle/icict/43693 |
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1553-7374 |
| dc.identifier.doi.none.fl_str_mv |
10.1371/journal.ppat.1008781 |
| identifier_str_mv |
OLIVEIRA, Antonio Edson R et al. Gene expression network analyses during infection with virulent and avirulentTrypanosoma cruzi strains unveil a role for fibroblasts in neutrophil recruitment and activation. PLoSPathog, v. 16, n. 8, e1008781, 2020. .https://doi.org/10.1371/journal.ppat.1008781 1553-7374 10.1371/journal.ppat.1008781 |
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Public Library of Science |
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Public Library of Science |
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