Inhibition of Trypanosoma cruzi proline racemase affects host-parasite interactions and the outcome of in vitro infection

Detalhes bibliográficos
Autor(a) principal: Coutinho, Leticia
Data de Publicação: 2009
Outros Autores: Ferreira, Marcelo Alves, Cosson, Alain, Batista, Marcos Meuser, Batista, Denise da Gama Jaén, Minoprio, Paola, Degrave, Win M., Berneman, Armand, Soeiro, Maria Nazaré C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/30969
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.
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spelling Coutinho, LeticiaFerreira, Marcelo AlvesCosson, AlainBatista, Marcos MeuserBatista, Denise da Gama JaénMinoprio, PaolaDegrave, Win M.Berneman, ArmandSoeiro, Maria Nazaré C.2019-01-10T15:19:10Z2019-01-10T15:19:10Z2009COUTINHO, Leticia et al. Inhibition of Trypanosoma cruzi proline racemase affects host-parasite interactions and the outcome of in vitro infection. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 104, n. 8, p. 1055-1062, Dec. 2009.0074-0276https://www.arca.fiocruz.br/handle/icict/309691678-8060engFundação Oswaldo Cruz. Instituto Oswaldo CruzTrypanosoma cruziProlina racemaseInibição enzimáticaInteração parasita-hospedeiroTrypanosoma cruziHost-parasite interactionEnzyme inhibitionProline racemaseInhibition of Trypanosoma cruzi proline racemase affects host-parasite interactions and the outcome of in vitro infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Institut Pasteur. Laboratoire d’Immunobiologie des infections à Trypanosoma. Département d’Immunologie. Paris, France.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.Institut Pasteur. Laboratoire d’Immunobiologie des infections à Trypanosoma. Département d’Immunologie. Paris, France.undação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Institut Pasteur. Laboratoire d’Immunobiologie des infections à Trypanosoma. Département d’Immunologie. Paris, France.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.Proline racemase is an important enzyme of Trypanosoma cruzi and has been shown to be an effective mitogen for B cells, thus contributing to the parasite’s immune evasion and persistence in the human host. Recombinant epimastigote parasites overexpressing TcPRAC genes coding for proline racemase present an augmented ability to differentiate into metacyclic infective forms and subsequently penetrate host-cells in vitro. Here we demonstrate that both anti T. cruzi proline racemase antibodies and the specific proline racemase inhibitor pyrrole-2-carboxylic acid significantly affect parasite infection of Vero cells in vitro. This inhibitor also hampers T. cruzi intracellular differentiation.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/30969/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALleticia_coutinho_etal_IOC_2009.pdfleticia_coutinho_etal_IOC_2009.pdfapplication/pdf619523https://www.arca.fiocruz.br/bitstream/icict/30969/2/leticia_coutinho_etal_IOC_2009.pdf7b588428a6a54d8082f550dacc4b92e9MD52TEXTleticia_coutinho_etal_IOC_2009.pdf.txtleticia_coutinho_etal_IOC_2009.pdf.txtExtracted texttext/plain40163https://www.arca.fiocruz.br/bitstream/icict/30969/3/leticia_coutinho_etal_IOC_2009.pdf.txt2740254ae0709f850f29f7354419f8f0MD53icict/309692023-09-14 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dc.title.pt_BR.fl_str_mv Inhibition of Trypanosoma cruzi proline racemase affects host-parasite interactions and the outcome of in vitro infection
title Inhibition of Trypanosoma cruzi proline racemase affects host-parasite interactions and the outcome of in vitro infection
spellingShingle Inhibition of Trypanosoma cruzi proline racemase affects host-parasite interactions and the outcome of in vitro infection
Coutinho, Leticia
Trypanosoma cruzi
Prolina racemase
Inibição enzimática
Interação parasita-hospedeiro
Trypanosoma cruzi
Host-parasite interaction
Enzyme inhibition
Proline racemase
title_short Inhibition of Trypanosoma cruzi proline racemase affects host-parasite interactions and the outcome of in vitro infection
title_full Inhibition of Trypanosoma cruzi proline racemase affects host-parasite interactions and the outcome of in vitro infection
title_fullStr Inhibition of Trypanosoma cruzi proline racemase affects host-parasite interactions and the outcome of in vitro infection
title_full_unstemmed Inhibition of Trypanosoma cruzi proline racemase affects host-parasite interactions and the outcome of in vitro infection
title_sort Inhibition of Trypanosoma cruzi proline racemase affects host-parasite interactions and the outcome of in vitro infection
author Coutinho, Leticia
author_facet Coutinho, Leticia
Ferreira, Marcelo Alves
Cosson, Alain
Batista, Marcos Meuser
Batista, Denise da Gama Jaén
Minoprio, Paola
Degrave, Win M.
Berneman, Armand
Soeiro, Maria Nazaré C.
author_role author
author2 Ferreira, Marcelo Alves
Cosson, Alain
Batista, Marcos Meuser
Batista, Denise da Gama Jaén
Minoprio, Paola
Degrave, Win M.
Berneman, Armand
Soeiro, Maria Nazaré C.
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Coutinho, Leticia
Ferreira, Marcelo Alves
Cosson, Alain
Batista, Marcos Meuser
Batista, Denise da Gama Jaén
Minoprio, Paola
Degrave, Win M.
Berneman, Armand
Soeiro, Maria Nazaré C.
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
Prolina racemase
Inibição enzimática
Interação parasita-hospedeiro
topic Trypanosoma cruzi
Prolina racemase
Inibição enzimática
Interação parasita-hospedeiro
Trypanosoma cruzi
Host-parasite interaction
Enzyme inhibition
Proline racemase
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
Host-parasite interaction
Enzyme inhibition
Proline racemase
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2019-01-10T15:19:10Z
dc.date.available.fl_str_mv 2019-01-10T15:19:10Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv COUTINHO, Leticia et al. Inhibition of Trypanosoma cruzi proline racemase affects host-parasite interactions and the outcome of in vitro infection. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 104, n. 8, p. 1055-1062, Dec. 2009.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/30969
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv COUTINHO, Leticia et al. Inhibition of Trypanosoma cruzi proline racemase affects host-parasite interactions and the outcome of in vitro infection. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 104, n. 8, p. 1055-1062, Dec. 2009.
0074-0276
1678-8060
url https://www.arca.fiocruz.br/handle/icict/30969
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/30969/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/30969/2/leticia_coutinho_etal_IOC_2009.pdf
https://www.arca.fiocruz.br/bitstream/icict/30969/3/leticia_coutinho_etal_IOC_2009.pdf.txt
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