Inhibition of Trypanosoma cruzi proline racemase affects host-parasite interactions and the outcome of in vitro infection

Detalhes bibliográficos
Autor(a) principal: Coutinho,Leticia
Data de Publicação: 2009
Outros Autores: Ferreira,Marcelo Alves, Cosson,Alain, Batista,Marcos Meuser, Batista,Denise da Gama Jaén, Minoprio,Paola, Degrave,Wim M, Berneman,Armand, Soeiro,Maria de Nazaré Correia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000800001
Resumo: Proline racemase is an important enzyme of Trypanosoma cruzi and has been shown to be an effective mitogen for B cells, thus contributing to the parasite's immune evasion and persistence in the human host. Recombinant epimastigote parasites overexpressing TcPRAC genes coding for proline racemase present an augmented ability to differentiate into metacyclic infective forms and subsequently penetrate host-cells in vitro. Here we demonstrate that both anti T. cruzi proline racemase antibodies and the specific proline racemase inhibitor pyrrole-2-carboxylic acid significantly affect parasite infection of Vero cells in vitro. This inhibitor also hampers T. cruzi intracellular differentiation.
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spelling Inhibition of Trypanosoma cruzi proline racemase affects host-parasite interactions and the outcome of in vitro infectionTrypanosoma cruziproline racemasehost-parasite interactionenzyme inhibitionProline racemase is an important enzyme of Trypanosoma cruzi and has been shown to be an effective mitogen for B cells, thus contributing to the parasite's immune evasion and persistence in the human host. Recombinant epimastigote parasites overexpressing TcPRAC genes coding for proline racemase present an augmented ability to differentiate into metacyclic infective forms and subsequently penetrate host-cells in vitro. Here we demonstrate that both anti T. cruzi proline racemase antibodies and the specific proline racemase inhibitor pyrrole-2-carboxylic acid significantly affect parasite infection of Vero cells in vitro. This inhibitor also hampers T. cruzi intracellular differentiation.Instituto Oswaldo Cruz, Ministério da Saúde2009-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000800001Memórias do Instituto Oswaldo Cruz v.104 n.8 2009reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762009000800001info:eu-repo/semantics/openAccessCoutinho,LeticiaFerreira,Marcelo AlvesCosson,AlainBatista,Marcos MeuserBatista,Denise da Gama JaénMinoprio,PaolaDegrave,Wim MBerneman,ArmandSoeiro,Maria de Nazaré Correiaeng2020-04-25T17:50:34Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:16:31.625Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Inhibition of Trypanosoma cruzi proline racemase affects host-parasite interactions and the outcome of in vitro infection
title Inhibition of Trypanosoma cruzi proline racemase affects host-parasite interactions and the outcome of in vitro infection
spellingShingle Inhibition of Trypanosoma cruzi proline racemase affects host-parasite interactions and the outcome of in vitro infection
Coutinho,Leticia
Trypanosoma cruzi
proline racemase
host-parasite interaction
enzyme inhibition
title_short Inhibition of Trypanosoma cruzi proline racemase affects host-parasite interactions and the outcome of in vitro infection
title_full Inhibition of Trypanosoma cruzi proline racemase affects host-parasite interactions and the outcome of in vitro infection
title_fullStr Inhibition of Trypanosoma cruzi proline racemase affects host-parasite interactions and the outcome of in vitro infection
title_full_unstemmed Inhibition of Trypanosoma cruzi proline racemase affects host-parasite interactions and the outcome of in vitro infection
title_sort Inhibition of Trypanosoma cruzi proline racemase affects host-parasite interactions and the outcome of in vitro infection
author Coutinho,Leticia
author_facet Coutinho,Leticia
Ferreira,Marcelo Alves
Cosson,Alain
Batista,Marcos Meuser
Batista,Denise da Gama Jaén
Minoprio,Paola
Degrave,Wim M
Berneman,Armand
Soeiro,Maria de Nazaré Correia
author_role author
author2 Ferreira,Marcelo Alves
Cosson,Alain
Batista,Marcos Meuser
Batista,Denise da Gama Jaén
Minoprio,Paola
Degrave,Wim M
Berneman,Armand
Soeiro,Maria de Nazaré Correia
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Coutinho,Leticia
Ferreira,Marcelo Alves
Cosson,Alain
Batista,Marcos Meuser
Batista,Denise da Gama Jaén
Minoprio,Paola
Degrave,Wim M
Berneman,Armand
Soeiro,Maria de Nazaré Correia
dc.subject.por.fl_str_mv Trypanosoma cruzi
proline racemase
host-parasite interaction
enzyme inhibition
topic Trypanosoma cruzi
proline racemase
host-parasite interaction
enzyme inhibition
dc.description.none.fl_txt_mv Proline racemase is an important enzyme of Trypanosoma cruzi and has been shown to be an effective mitogen for B cells, thus contributing to the parasite's immune evasion and persistence in the human host. Recombinant epimastigote parasites overexpressing TcPRAC genes coding for proline racemase present an augmented ability to differentiate into metacyclic infective forms and subsequently penetrate host-cells in vitro. Here we demonstrate that both anti T. cruzi proline racemase antibodies and the specific proline racemase inhibitor pyrrole-2-carboxylic acid significantly affect parasite infection of Vero cells in vitro. This inhibitor also hampers T. cruzi intracellular differentiation.
description Proline racemase is an important enzyme of Trypanosoma cruzi and has been shown to be an effective mitogen for B cells, thus contributing to the parasite's immune evasion and persistence in the human host. Recombinant epimastigote parasites overexpressing TcPRAC genes coding for proline racemase present an augmented ability to differentiate into metacyclic infective forms and subsequently penetrate host-cells in vitro. Here we demonstrate that both anti T. cruzi proline racemase antibodies and the specific proline racemase inhibitor pyrrole-2-carboxylic acid significantly affect parasite infection of Vero cells in vitro. This inhibitor also hampers T. cruzi intracellular differentiation.
publishDate 2009
dc.date.none.fl_str_mv 2009-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000800001
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000800001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762009000800001
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.104 n.8 2009
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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