Expression of Hepatitis B Virus Surface Antigen (HBsAg) from Genotypes A, D and F and Influence of Amino Acid Variations Related or Not to Genotypes on HBsAg Detection

Detalhes bibliográficos
Autor(a) principal: Araujo, Natalia M.
Data de Publicação: 2009
Outros Autores: Vianna, Carlos O. A., Moraes, Marcia T. B., Gomes, Selma A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/27720
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ. Brasil.
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spelling Araujo, Natalia M.Vianna, Carlos O. A.Moraes, Marcia T. B.Gomes, Selma A.2018-07-30T17:44:12Z2018-07-30T17:44:12Z2009ARAUJO, Natalia M. et al. Expression of Hepatitis B Virus Surface Antigen (HBsAg) from Genotypes A, D and F and Influence of Amino Acid Variations Related or Not to Genotypes on HBsAg Detection. The Brazilian Journal of Infectious Diseases, v. 13, n. 4, p. 266-271, 2009.1413-8670https://www.arca.fiocruz.br/handle/icict/2772010.1590/S1413-867020090004000051678-4391engElsevierVirus da Hepatite BAntígeno de superfícieTransfecçãoVariações de aminoácidosHepatitis B virusSurface antigenAmino acid variationsTransfectionExpression of Hepatitis B Virus Surface Antigen (HBsAg) from Genotypes A, D and F and Influence of Amino Acid Variations Related or Not to Genotypes on HBsAg Detectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Vice Direção de Desenvolvimento Tecnológico. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia Recombinante. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ. Brasil.The impact of hepatitis B virus (HBV) genotypes on the sensitivity of surface antigen (HBsAg) detection assays has been poorly investigated. Here, plasmids carrying consensus or variant coding sequences for HBV surface proteins from genotypes A, D and F, were constructed. HBsAg levels were evaluated in medium and extracts of transfected CHO cells by a commercial polyclonal-based assay. We show that HBsAg detection values of consensus forms from genotypes D and F were, respectively, 37% and 30% lower than those obtained by genotype A. However, the presence of two single variations, T143M in genotype A, and T125M in genotype D, produced a decrease of 44% and an increase of 34%, respectively, on HBsAg mean values in comparison with their consensus forms. In conclusion, HBsAg detection levels varied among HBV genotypes. However, unique amino acid substitutions not linked to genotypes, such as T125M and T143M described here, should have more implications in HBV immunological diagnostics than the set of variations characteristic of each HBV genotype.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/27720/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALnataliam_araujo_etal_IOC_2009.pdfnataliam_araujo_etal_IOC_2009.pdfapplication/pdf500445https://www.arca.fiocruz.br/bitstream/icict/27720/2/nataliam_araujo_etal_IOC_2009.pdfe38f1b87d6f3d8cd28d8ca0681bf5edaMD52TEXTnataliam_araujo_etal_IOC_2009.pdf.txtnataliam_araujo_etal_IOC_2009.pdf.txtExtracted texttext/plain25297https://www.arca.fiocruz.br/bitstream/icict/27720/3/nataliam_araujo_etal_IOC_2009.pdf.txt58d60ce4ae3b2c590ec460f41464b0e6MD53icict/277202019-04-05 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dc.title.pt_BR.fl_str_mv Expression of Hepatitis B Virus Surface Antigen (HBsAg) from Genotypes A, D and F and Influence of Amino Acid Variations Related or Not to Genotypes on HBsAg Detection
title Expression of Hepatitis B Virus Surface Antigen (HBsAg) from Genotypes A, D and F and Influence of Amino Acid Variations Related or Not to Genotypes on HBsAg Detection
spellingShingle Expression of Hepatitis B Virus Surface Antigen (HBsAg) from Genotypes A, D and F and Influence of Amino Acid Variations Related or Not to Genotypes on HBsAg Detection
Araujo, Natalia M.
Virus da Hepatite B
Antígeno de superfície
Transfecção
Variações de aminoácidos
Hepatitis B virus
Surface antigen
Amino acid variations
Transfection
title_short Expression of Hepatitis B Virus Surface Antigen (HBsAg) from Genotypes A, D and F and Influence of Amino Acid Variations Related or Not to Genotypes on HBsAg Detection
title_full Expression of Hepatitis B Virus Surface Antigen (HBsAg) from Genotypes A, D and F and Influence of Amino Acid Variations Related or Not to Genotypes on HBsAg Detection
title_fullStr Expression of Hepatitis B Virus Surface Antigen (HBsAg) from Genotypes A, D and F and Influence of Amino Acid Variations Related or Not to Genotypes on HBsAg Detection
title_full_unstemmed Expression of Hepatitis B Virus Surface Antigen (HBsAg) from Genotypes A, D and F and Influence of Amino Acid Variations Related or Not to Genotypes on HBsAg Detection
title_sort Expression of Hepatitis B Virus Surface Antigen (HBsAg) from Genotypes A, D and F and Influence of Amino Acid Variations Related or Not to Genotypes on HBsAg Detection
author Araujo, Natalia M.
author_facet Araujo, Natalia M.
Vianna, Carlos O. A.
Moraes, Marcia T. B.
Gomes, Selma A.
author_role author
author2 Vianna, Carlos O. A.
Moraes, Marcia T. B.
Gomes, Selma A.
author2_role author
author
author
dc.contributor.author.fl_str_mv Araujo, Natalia M.
Vianna, Carlos O. A.
Moraes, Marcia T. B.
Gomes, Selma A.
dc.subject.other.pt_BR.fl_str_mv Virus da Hepatite B
Antígeno de superfície
Transfecção
Variações de aminoácidos
topic Virus da Hepatite B
Antígeno de superfície
Transfecção
Variações de aminoácidos
Hepatitis B virus
Surface antigen
Amino acid variations
Transfection
dc.subject.en.pt_BR.fl_str_mv Hepatitis B virus
Surface antigen
Amino acid variations
Transfection
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ. Brasil.
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2018-07-30T17:44:12Z
dc.date.available.fl_str_mv 2018-07-30T17:44:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ARAUJO, Natalia M. et al. Expression of Hepatitis B Virus Surface Antigen (HBsAg) from Genotypes A, D and F and Influence of Amino Acid Variations Related or Not to Genotypes on HBsAg Detection. The Brazilian Journal of Infectious Diseases, v. 13, n. 4, p. 266-271, 2009.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/27720
dc.identifier.issn.pt_BR.fl_str_mv 1413-8670
dc.identifier.doi.pt_BR.fl_str_mv 10.1590/S1413-86702009000400005
dc.identifier.eissn.none.fl_str_mv 1678-4391
identifier_str_mv ARAUJO, Natalia M. et al. Expression of Hepatitis B Virus Surface Antigen (HBsAg) from Genotypes A, D and F and Influence of Amino Acid Variations Related or Not to Genotypes on HBsAg Detection. The Brazilian Journal of Infectious Diseases, v. 13, n. 4, p. 266-271, 2009.
1413-8670
10.1590/S1413-86702009000400005
1678-4391
url https://www.arca.fiocruz.br/handle/icict/27720
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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