In vitro initial immune response against Leishmania amazonensis infection is characterized by an increased production of IL-10 and IL-13

Detalhes bibliográficos
Autor(a) principal: Coêlho, Zirlane Castelo Branco
Data de Publicação: 2010
Outros Autores: Teixeira, Maria Jânia, Mota, Erika Freitas, Frutuoso, Mércia Sindeaux, Silva, João Santana da, Barral, Aldina Maria Prado, Barral Netto, Manoel, Pompeu, Margarida Maria de Lima
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7399
Resumo: Universidade Federal do Ceará. Medical School. Fortaleza, CE, Brasil
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spelling Coêlho, Zirlane Castelo BrancoTeixeira, Maria JâniaMota, Erika FreitasFrutuoso, Mércia SindeauxSilva, João Santana daBarral, Aldina Maria PradoBarral Netto, ManoelPompeu, Margarida Maria de Lima2014-03-12T19:12:11Z2014-03-12T19:12:11Z2010COELHO, Z. C. B. et al. In vitro initial immune response against Leishmania amazonensis infection is characterized by an increased production of IL-10 and IL-13. Brazilian Journal of Infectious Diseases, v. 14, n. 5, p.472-482, 2010.1678-4391https://www.arca.fiocruz.br/handle/icict/7399engElsevier Editora LtdaIn vitro initial immune response against Leishmania amazonensis infection is characterized by an increased production of IL-10 and IL-13info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Ceará. Medical School. Fortaleza, CE, BrasilUniversidade Federal do Ceará. Medical School. Fortaleza, CE, BrasilUniversidade Federal do Ceará. Medical School. Fortaleza, CE, BrasilUniversidade Federal do Ceará. Medical School. Fortaleza, CE, BrasilUniversidade de São Paulo. Medical School of Ribeirão Preto. São Paulo, SP, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal do Ceará. Medical School. Fortaleza, CE, BrasilThe initial encounter of Leishmania with its host's immune system is important in the outcome of infection. Previous studies have shown that PBMCs from healthy volunteers (HV) exposed to Leishmania differ in IFN-γ production. We have expanded such observations evaluating the profile and kinetics of cytokines (IFN-γ, IL-12p70, IL-10, IL-13), chemokines (CCL5, CCL3, CCL4, CXCL10), and chemokine receptors (CCR1,CCR5, CXCR3, CCR4) in vitro L. amazonensis-stimulated of HV's PBMCs. HVs were divided in groups of high (HR) or low (LR) IFN-γ responders. In both groups, HR and LR, after L. amazonensis infection there was a predominance of IL-10 and IL-13 over IFN-γ production, while IL-12 was produced in similar amount. Regarding chemokines, a more striking difference was observed for CCL3 expression that was lower at 12 hours and 48 hours post infection in LR than in HR. Interestingly, a downregulation of CCR5 and a greater expression of CCR4 were found in low IFN-γ responders. These data suggest that early after L. amazonensis infection there is a cytokine milieu dominated by IL-13 and IL-10, and despite of this environment, IFN-γ is produced, supporting the complexity of the response. It is noteworthy that the pattern of immune response is mounted in first hours after Leishmania stimulation, with the definition of the differentiation of Th1 versus Th2 cells. It remains to be determined if such an in vitro difference has an in vivo counterpart in terms of susceptibility to infection.Leishmania amazonensisInterleukin-10Interleukin-13Interações Hospedeiro-Parasita/imunologiaImunidade Humoral/imunologiaInterleucina-10/biossínteseInterleucina-13/biossínteseLeishmania mexicana/imunologiaLeucócitos Mononucleares/parasitologiaCitocinas/biossínteseDNA de Protozoário/análiseEnsaio de Imunoadsorção EnzimáticaHumanosInterleucina-10/imunologiaInterleucina-13/imunologiaLeishmania mexicana/fisiologiaLeucócitos Mononucleares/imunologiaRNA de Protozoário/análiseReação em Cadeia da Polimerase Via Transcriptase Reversainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALCoelho Z C B In vitro initial....pdfCoelho Z C B In vitro initial....pdfapplication/pdf1046227https://www.arca.fiocruz.br/bitstream/icict/7399/1/Coelho%20Z%20C%20B%20In%20vitro%20initial....pdf0777355c1e9995b41efa9df842cd23c8MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7399/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTCoelho Z C B In vitro initial....pdf.txtCoelho Z C B In vitro initial....pdf.txtExtracted texttext/plain35354https://www.arca.fiocruz.br/bitstream/icict/7399/5/Coelho%20Z%20C%20B%20In%20vitro%20initial....pdf.txtdd04bddad3ce9ee56060bb1608899989MD55THUMBNAILCoelho Z C B In vitro initial....pdf.jpgCoelho Z C B In vitro initial....pdf.jpgGenerated Thumbnailimage/jpeg2153https://www.arca.fiocruz.br/bitstream/icict/7399/4/Coelho%20Z%20C%20B%20In%20vitro%20initial....pdf.jpgb78447c309b4c6e691a60ef4da4b3b26MD54icict/73992023-03-15 14:33:26.701oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:26Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv In vitro initial immune response against Leishmania amazonensis infection is characterized by an increased production of IL-10 and IL-13
title In vitro initial immune response against Leishmania amazonensis infection is characterized by an increased production of IL-10 and IL-13
spellingShingle In vitro initial immune response against Leishmania amazonensis infection is characterized by an increased production of IL-10 and IL-13
Coêlho, Zirlane Castelo Branco
Leishmania amazonensis
Interleukin-10
Interleukin-13
Interações Hospedeiro-Parasita/imunologia
Imunidade Humoral/imunologia
Interleucina-10/biossíntese
Interleucina-13/biossíntese
Leishmania mexicana/imunologia
Leucócitos Mononucleares/parasitologia
Citocinas/biossíntese
DNA de Protozoário/análise
Ensaio de Imunoadsorção Enzimática
Humanos
Interleucina-10/imunologia
Interleucina-13/imunologia
Leishmania mexicana/fisiologia
Leucócitos Mononucleares/imunologia
RNA de Protozoário/análise
Reação em Cadeia da Polimerase Via Transcriptase Reversa
title_short In vitro initial immune response against Leishmania amazonensis infection is characterized by an increased production of IL-10 and IL-13
title_full In vitro initial immune response against Leishmania amazonensis infection is characterized by an increased production of IL-10 and IL-13
title_fullStr In vitro initial immune response against Leishmania amazonensis infection is characterized by an increased production of IL-10 and IL-13
title_full_unstemmed In vitro initial immune response against Leishmania amazonensis infection is characterized by an increased production of IL-10 and IL-13
title_sort In vitro initial immune response against Leishmania amazonensis infection is characterized by an increased production of IL-10 and IL-13
author Coêlho, Zirlane Castelo Branco
author_facet Coêlho, Zirlane Castelo Branco
Teixeira, Maria Jânia
Mota, Erika Freitas
Frutuoso, Mércia Sindeaux
Silva, João Santana da
Barral, Aldina Maria Prado
Barral Netto, Manoel
Pompeu, Margarida Maria de Lima
author_role author
author2 Teixeira, Maria Jânia
Mota, Erika Freitas
Frutuoso, Mércia Sindeaux
Silva, João Santana da
Barral, Aldina Maria Prado
Barral Netto, Manoel
Pompeu, Margarida Maria de Lima
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Coêlho, Zirlane Castelo Branco
Teixeira, Maria Jânia
Mota, Erika Freitas
Frutuoso, Mércia Sindeaux
Silva, João Santana da
Barral, Aldina Maria Prado
Barral Netto, Manoel
Pompeu, Margarida Maria de Lima
dc.subject.en.pt_BR.fl_str_mv Leishmania amazonensis
Interleukin-10
Interleukin-13
topic Leishmania amazonensis
Interleukin-10
Interleukin-13
Interações Hospedeiro-Parasita/imunologia
Imunidade Humoral/imunologia
Interleucina-10/biossíntese
Interleucina-13/biossíntese
Leishmania mexicana/imunologia
Leucócitos Mononucleares/parasitologia
Citocinas/biossíntese
DNA de Protozoário/análise
Ensaio de Imunoadsorção Enzimática
Humanos
Interleucina-10/imunologia
Interleucina-13/imunologia
Leishmania mexicana/fisiologia
Leucócitos Mononucleares/imunologia
RNA de Protozoário/análise
Reação em Cadeia da Polimerase Via Transcriptase Reversa
dc.subject.decs.pt_BR.fl_str_mv Interações Hospedeiro-Parasita/imunologia
Imunidade Humoral/imunologia
Interleucina-10/biossíntese
Interleucina-13/biossíntese
Leishmania mexicana/imunologia
Leucócitos Mononucleares/parasitologia
Citocinas/biossíntese
DNA de Protozoário/análise
Ensaio de Imunoadsorção Enzimática
Humanos
Interleucina-10/imunologia
Interleucina-13/imunologia
Leishmania mexicana/fisiologia
Leucócitos Mononucleares/imunologia
RNA de Protozoário/análise
Reação em Cadeia da Polimerase Via Transcriptase Reversa
description Universidade Federal do Ceará. Medical School. Fortaleza, CE, Brasil
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2014-03-12T19:12:11Z
dc.date.available.fl_str_mv 2014-03-12T19:12:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv COELHO, Z. C. B. et al. In vitro initial immune response against Leishmania amazonensis infection is characterized by an increased production of IL-10 and IL-13. Brazilian Journal of Infectious Diseases, v. 14, n. 5, p.472-482, 2010.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7399
dc.identifier.issn.none.fl_str_mv 1678-4391
identifier_str_mv COELHO, Z. C. B. et al. In vitro initial immune response against Leishmania amazonensis infection is characterized by an increased production of IL-10 and IL-13. Brazilian Journal of Infectious Diseases, v. 14, n. 5, p.472-482, 2010.
1678-4391
url https://www.arca.fiocruz.br/handle/icict/7399
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier Editora Ltda
publisher.none.fl_str_mv Elsevier Editora Ltda
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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