Interleukin-12 restores interferon-gamma production and cytotoxic responses in visceral leishmaniasis

Detalhes bibliográficos
Autor(a) principal: Bacellar, Maria Olívia Amado Ramos
Data de Publicação: 1996
Outros Autores: Brodskyn, Claudia Ida, Guerreiro, Jaqueline, Barral Netto, Manoel, Costa, Carlos Henrique Nery, Coffman, Robert L, Johnson Junior, Warren D, Carvalho Filho, Edgar Marcelino
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7450
Resumo: Federal University of Bahia. Immunology Service of Hospital Universitario Professor Edgard Santos. Salvador, BA, Brasil
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spelling Bacellar, Maria Olívia Amado RamosBrodskyn, Claudia IdaGuerreiro, JaquelineBarral Netto, ManoelCosta, Carlos Henrique NeryCoffman, Robert LJohnson Junior, Warren DCarvalho Filho, Edgar Marcelino2014-03-26T14:27:51Z2014-03-26T14:27:51Z1996BACELLAR O. et al. Interleukin-12 restores interferon-gamma production and cytotoxic responses in visceral leishmaniasis. The Journal of Infectious Diseases, v. 173, n. 6, p. 1515-1518, 1996.0022-1899https://www.arca.fiocruz.br/handle/icict/7450engThe University of ChicagoInterleukin-12 restores interferon-gamma production and cytotoxic responses in visceral leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFederal University of Bahia. Immunology Service of Hospital Universitario Professor Edgard Santos. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Mycobacteria. Salvador, BA, BrasilFederal University of Piaui. Hospital de Doencas Infecto-Contagiosas. Teresina, PI, BrazilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Mycobacteria. Salvador, BA, BrasilFederal University of Piaui. Hospital de Doencas Infecto-Contagiosas. Teresina, PI, BrazilDNAX Research Institute. Department of Immunology.Palo Alto, CaliforniaCornell University Medical College. Division of International Medicine and Infectious Diseases. New York, New YorkFederal University of Bahia. Immunology Service of Hospital Universitario Professor Edgard Santos. Salvador, BA, BrasilAmerican visceral leishmaniasis (AVL) is associated with the absence of lymphocyte proliferative responses and interleukin (IL)-2 and interferon-y (IFN-y) production upon stimulation with Leishmania antigen. In contrast, cure of AVL is associated with restoration of these T cell functions. In the present study, the ability of IL-12, a cytokine that acts on NK and T cells to restore cellular immune responses in AVL, was evaluated. Participants of the study included 12 patients with AVL and 7 subjects cured of AVL. The [3H]thymidine uptake and IFN-y production in cultures of peripheral blood mononuclear cells (from AVLpatients) stimulated with Leishmaniachagasi antigen were 882 ± 1393cpm and zero, respectively. Addition of IL-12 enhanced the proliferative response to 5097 ± 6429 cpm (P < .001) and IFN-y production to 305 ± 325 pglmL (P < .01). IL-12 also restored cytotoxic activity against the K562 cell line. These results indicate that IL-12 has an important role in the regulation of the cellular immune response in human leishmaniasis.Citotoxicidade Imunológica/imunologiaInterferon gama/biossínteseInterleucina-12/farmacologiaLeishmaniose Visceral/imunologiaAtivação Linfocitária/imunologiaAnimaisAntígenos de Protozoários/imunologiaCélulas CultivadasDNA/biossínteseHumanosInterleucina-10/imunologiaLeishmania infantum/imunologiaLeucócitos Mononucleares/imunologiaProteínas Recombinantes/farmacologiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALBacellar O, Interleukin-12 restores....pdfBacellar O, Interleukin-12 restores....pdfapplication/pdf377896https://www.arca.fiocruz.br/bitstream/icict/7450/1/Bacellar%20O%2c%20Interleukin-12%20restores....pdfe6cf268a43f27edefa85fb46c0f9ae8eMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7450/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTBacellar O, Interleukin-12 restores....pdf.txtBacellar O, Interleukin-12 restores....pdf.txtExtracted texttext/plain18700https://www.arca.fiocruz.br/bitstream/icict/7450/5/Bacellar%20O%2c%20Interleukin-12%20restores....pdf.txtdc5896aef83471edb4ba37d504b33a4fMD55THUMBNAILBacellar O, Interleukin-12 restores....pdf.jpgBacellar O, Interleukin-12 restores....pdf.jpgGenerated Thumbnailimage/jpeg2673https://www.arca.fiocruz.br/bitstream/icict/7450/4/Bacellar%20O%2c%20Interleukin-12%20restores....pdf.jpgd3bfe8f1b26a78cc9823a6cbe7d9018cMD54icict/74502023-03-15 14:33:02.762oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:02Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Interleukin-12 restores interferon-gamma production and cytotoxic responses in visceral leishmaniasis
title Interleukin-12 restores interferon-gamma production and cytotoxic responses in visceral leishmaniasis
spellingShingle Interleukin-12 restores interferon-gamma production and cytotoxic responses in visceral leishmaniasis
Bacellar, Maria Olívia Amado Ramos
Citotoxicidade Imunológica/imunologia
Interferon gama/biossíntese
Interleucina-12/farmacologia
Leishmaniose Visceral/imunologia
Ativação Linfocitária/imunologia
Animais
Antígenos de Protozoários/imunologia
Células Cultivadas
DNA/biossíntese
Humanos
Interleucina-10/imunologia
Leishmania infantum/imunologia
Leucócitos Mononucleares/imunologia
Proteínas Recombinantes/farmacologia
title_short Interleukin-12 restores interferon-gamma production and cytotoxic responses in visceral leishmaniasis
title_full Interleukin-12 restores interferon-gamma production and cytotoxic responses in visceral leishmaniasis
title_fullStr Interleukin-12 restores interferon-gamma production and cytotoxic responses in visceral leishmaniasis
title_full_unstemmed Interleukin-12 restores interferon-gamma production and cytotoxic responses in visceral leishmaniasis
title_sort Interleukin-12 restores interferon-gamma production and cytotoxic responses in visceral leishmaniasis
author Bacellar, Maria Olívia Amado Ramos
author_facet Bacellar, Maria Olívia Amado Ramos
Brodskyn, Claudia Ida
Guerreiro, Jaqueline
Barral Netto, Manoel
Costa, Carlos Henrique Nery
Coffman, Robert L
Johnson Junior, Warren D
Carvalho Filho, Edgar Marcelino
author_role author
author2 Brodskyn, Claudia Ida
Guerreiro, Jaqueline
Barral Netto, Manoel
Costa, Carlos Henrique Nery
Coffman, Robert L
Johnson Junior, Warren D
Carvalho Filho, Edgar Marcelino
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Bacellar, Maria Olívia Amado Ramos
Brodskyn, Claudia Ida
Guerreiro, Jaqueline
Barral Netto, Manoel
Costa, Carlos Henrique Nery
Coffman, Robert L
Johnson Junior, Warren D
Carvalho Filho, Edgar Marcelino
dc.subject.decs.pt_BR.fl_str_mv Citotoxicidade Imunológica/imunologia
Interferon gama/biossíntese
Interleucina-12/farmacologia
Leishmaniose Visceral/imunologia
Ativação Linfocitária/imunologia
Animais
Antígenos de Protozoários/imunologia
Células Cultivadas
DNA/biossíntese
Humanos
Interleucina-10/imunologia
Leishmania infantum/imunologia
Leucócitos Mononucleares/imunologia
Proteínas Recombinantes/farmacologia
topic Citotoxicidade Imunológica/imunologia
Interferon gama/biossíntese
Interleucina-12/farmacologia
Leishmaniose Visceral/imunologia
Ativação Linfocitária/imunologia
Animais
Antígenos de Protozoários/imunologia
Células Cultivadas
DNA/biossíntese
Humanos
Interleucina-10/imunologia
Leishmania infantum/imunologia
Leucócitos Mononucleares/imunologia
Proteínas Recombinantes/farmacologia
description Federal University of Bahia. Immunology Service of Hospital Universitario Professor Edgard Santos. Salvador, BA, Brasil
publishDate 1996
dc.date.issued.fl_str_mv 1996
dc.date.accessioned.fl_str_mv 2014-03-26T14:27:51Z
dc.date.available.fl_str_mv 2014-03-26T14:27:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BACELLAR O. et al. Interleukin-12 restores interferon-gamma production and cytotoxic responses in visceral leishmaniasis. The Journal of Infectious Diseases, v. 173, n. 6, p. 1515-1518, 1996.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7450
dc.identifier.issn.none.fl_str_mv 0022-1899
identifier_str_mv BACELLAR O. et al. Interleukin-12 restores interferon-gamma production and cytotoxic responses in visceral leishmaniasis. The Journal of Infectious Diseases, v. 173, n. 6, p. 1515-1518, 1996.
0022-1899
url https://www.arca.fiocruz.br/handle/icict/7450
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv The University of Chicago
publisher.none.fl_str_mv The University of Chicago
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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institution FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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