Ultrastructural localization of Hepatitis C virus positive and negative strand RNA and proteins in Hepatocytes of a Rhesus Monkey (Macaca Mulatta)
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2010 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/16884 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil. |
| id |
CRUZ_8af2bf88fdd4c37e6b27be8442d9a506 |
|---|---|
| oai_identifier_str |
oai:www.arca.fiocruz.br:icict/16884 |
| network_acronym_str |
CRUZ |
| network_name_str |
Repositório Institucional da FIOCRUZ (ARCA) |
| repository_id_str |
2135 |
| spelling |
Majerowicz, SelmaGrief, ChristopherPinto, Marcelo AlvesBaptista, Marcia LeitePinhão, Angela TeixeiraAirano, Renata CristinaVitral, Cláudia LamarcaMarchevsky, Renato SergioYoshida, Clara F. T.Barth, Ortrud Monika2017-01-18T11:35:29Z2017-01-18T11:35:29Z2010MAJEROWICZ, Selma; et al. Ultrastructural localization of Hepatitis C Virus positive and negative strand RNA and proteins in Hepatocytes of a Rhesus Monkey (Macaca Mulatta). Virus Reviews & Research, v.14, n.1, 16p, 2009.1519-2563https://www.arca.fiocruz.br/handle/icict/168842357-9323engBrazilian Society of VirologyVírus da hepatite CMacaco rhesusUltraestruturaHibridização in situProteínas viraisrhesus monkeyhepatitis C virusultrastructurein situ hybridizationviral proteinsreplication complexUltrastructural localization of Hepatitis C virus positive and negative strand RNA and proteins in Hepatocytes of a Rhesus Monkey (Macaca Mulatta)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil / NIBSC. Potters Bar. Herts, EN6 3QG, Inglaterra.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.Universidade Federal Fluminense. Instituto Biomédico. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.Morphological alterations of the liver from rhesus macaque (Macaca mulatta) experimentally infected with hepatitis C virus (HCV) were analyzed using electron microscopy. The localization of viral RNA and proteins inside hepatocytes was demonstrated using in situ hybridization and immunoelectron microscopy techniques. The animals were inoculated by different routes. The infection was successful only by use of the intrasplenic approach to HCV infected autogenic hepatocyte transplant. The inoculum used to infect the hepatocytes was characterized as genotype 3 with 107 RNA copies/mL. In situ hybridization was performed using a complementary negative and positive strand probe made with the specific primer. Despite that the level of HCV infection was considered to be low, we were able to detect and localize viral positive and negative RNA strands and viral proteins in altered membranes of the rough endoplasmic reticulum in infected liver cells, showing evidence of viral replication in vivo.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/16884/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALselma_majerowicz_etal_IOC_2010.pdfselma_majerowicz_etal_IOC_2010.pdfapplication/pdf1093154https://www.arca.fiocruz.br/bitstream/icict/16884/2/selma_majerowicz_etal_IOC_2010.pdff2cd42822ebaee8c5987159bbe82f820MD52TEXTselma_majerowicz_etal_IOC_2010.pdf.txtselma_majerowicz_etal_IOC_2010.pdf.txtExtracted texttext/plain30725https://www.arca.fiocruz.br/bitstream/icict/16884/3/selma_majerowicz_etal_IOC_2010.pdf.txtf8b19e29c55fe7f4baef3478fcb89ba7MD53icict/168842018-08-15 03:20:16.106oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-08-15T06:20:16Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Ultrastructural localization of Hepatitis C virus positive and negative strand RNA and proteins in Hepatocytes of a Rhesus Monkey (Macaca Mulatta) |
| title |
Ultrastructural localization of Hepatitis C virus positive and negative strand RNA and proteins in Hepatocytes of a Rhesus Monkey (Macaca Mulatta) |
| spellingShingle |
Ultrastructural localization of Hepatitis C virus positive and negative strand RNA and proteins in Hepatocytes of a Rhesus Monkey (Macaca Mulatta) Majerowicz, Selma Vírus da hepatite C Macaco rhesus Ultraestrutura Hibridização in situ Proteínas virais rhesus monkey hepatitis C virus ultrastructure in situ hybridization viral proteins replication complex |
| title_short |
Ultrastructural localization of Hepatitis C virus positive and negative strand RNA and proteins in Hepatocytes of a Rhesus Monkey (Macaca Mulatta) |
| title_full |
Ultrastructural localization of Hepatitis C virus positive and negative strand RNA and proteins in Hepatocytes of a Rhesus Monkey (Macaca Mulatta) |
| title_fullStr |
Ultrastructural localization of Hepatitis C virus positive and negative strand RNA and proteins in Hepatocytes of a Rhesus Monkey (Macaca Mulatta) |
| title_full_unstemmed |
Ultrastructural localization of Hepatitis C virus positive and negative strand RNA and proteins in Hepatocytes of a Rhesus Monkey (Macaca Mulatta) |
| title_sort |
Ultrastructural localization of Hepatitis C virus positive and negative strand RNA and proteins in Hepatocytes of a Rhesus Monkey (Macaca Mulatta) |
| author |
Majerowicz, Selma |
| author_facet |
Majerowicz, Selma Grief, Christopher Pinto, Marcelo Alves Baptista, Marcia Leite Pinhão, Angela Teixeira Airano, Renata Cristina Vitral, Cláudia Lamarca Marchevsky, Renato Sergio Yoshida, Clara F. T. Barth, Ortrud Monika |
| author_role |
author |
| author2 |
Grief, Christopher Pinto, Marcelo Alves Baptista, Marcia Leite Pinhão, Angela Teixeira Airano, Renata Cristina Vitral, Cláudia Lamarca Marchevsky, Renato Sergio Yoshida, Clara F. T. Barth, Ortrud Monika |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Majerowicz, Selma Grief, Christopher Pinto, Marcelo Alves Baptista, Marcia Leite Pinhão, Angela Teixeira Airano, Renata Cristina Vitral, Cláudia Lamarca Marchevsky, Renato Sergio Yoshida, Clara F. T. Barth, Ortrud Monika |
| dc.subject.other.pt_BR.fl_str_mv |
Vírus da hepatite C Macaco rhesus Ultraestrutura Hibridização in situ Proteínas virais |
| topic |
Vírus da hepatite C Macaco rhesus Ultraestrutura Hibridização in situ Proteínas virais rhesus monkey hepatitis C virus ultrastructure in situ hybridization viral proteins replication complex |
| dc.subject.en.pt_BR.fl_str_mv |
rhesus monkey hepatitis C virus ultrastructure in situ hybridization viral proteins replication complex |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil. |
| publishDate |
2010 |
| dc.date.issued.fl_str_mv |
2010 |
| dc.date.accessioned.fl_str_mv |
2017-01-18T11:35:29Z |
| dc.date.available.fl_str_mv |
2017-01-18T11:35:29Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.citation.fl_str_mv |
MAJEROWICZ, Selma; et al. Ultrastructural localization of Hepatitis C Virus positive and negative strand RNA and proteins in Hepatocytes of a Rhesus Monkey (Macaca Mulatta). Virus Reviews & Research, v.14, n.1, 16p, 2009. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/16884 |
| dc.identifier.issn.pt_BR.fl_str_mv |
1519-2563 |
| dc.identifier.eissn.none.fl_str_mv |
2357-9323 |
| identifier_str_mv |
MAJEROWICZ, Selma; et al. Ultrastructural localization of Hepatitis C Virus positive and negative strand RNA and proteins in Hepatocytes of a Rhesus Monkey (Macaca Mulatta). Virus Reviews & Research, v.14, n.1, 16p, 2009. 1519-2563 2357-9323 |
| url |
https://www.arca.fiocruz.br/handle/icict/16884 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
Brazilian Society of Virology |
| publisher.none.fl_str_mv |
Brazilian Society of Virology |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
| instname_str |
Fundação Oswaldo Cruz (FIOCRUZ) |
| instacron_str |
FIOCRUZ |
| institution |
FIOCRUZ |
| reponame_str |
Repositório Institucional da FIOCRUZ (ARCA) |
| collection |
Repositório Institucional da FIOCRUZ (ARCA) |
| bitstream.url.fl_str_mv |
https://www.arca.fiocruz.br/bitstream/icict/16884/1/license.txt https://www.arca.fiocruz.br/bitstream/icict/16884/2/selma_majerowicz_etal_IOC_2010.pdf https://www.arca.fiocruz.br/bitstream/icict/16884/3/selma_majerowicz_etal_IOC_2010.pdf.txt |
| bitstream.checksum.fl_str_mv |
5a560609d32a3863062d77ff32785d58 f2cd42822ebaee8c5987159bbe82f820 f8b19e29c55fe7f4baef3478fcb89ba7 |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
| repository.name.fl_str_mv |
Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ) |
| repository.mail.fl_str_mv |
repositorio.arca@fiocruz.br |
| _version_ |
1822791571042467840 |