In situ hybridization of hepatitis C virus RNA in liver cells of an experimentally infected rhesus macaque

Detalhes bibliográficos
Autor(a) principal: Majerowicz, Selma
Data de Publicação: 2004
Outros Autores: Grief, Christopher, Ferguson, Debora, Airano, Renata C., Baptista, Marcia L., Pinto, Marcelo A., Barth, Ortrud Monika
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/40270
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.
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spelling Majerowicz, SelmaGrief, ChristopherFerguson, DeboraAirano, Renata C.Baptista, Marcia L.Pinto, Marcelo A.Barth, Ortrud Monika2020-03-10T12:43:38Z2020-03-10T12:43:38Z2004MAJEROWICZ, Selma et al. In situ hybridization of hepatitis C virus RNA in liver cells of an experimentally infected rhesus macaque. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 99, n. 6, p. 629-631, Oct. 2004.0074-0206https://www.arca.fiocruz.br/handle/icict/4027010.1590/s0074-027620040006000171678-8060engFundação Oswaldo Cruz. Instituto Oswaldo CruzVírus da Hepatite CHibridização In SituMacaca mulattaIn situ hybridizationHepatitis C virusRhesus macaqueMacaca mulattaIn situ hybridization of hepatitis C virus RNA in liver cells of an experimentally infected rhesus macaqueinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.NIBSC, Potters Bar. Herts, UK.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.The liver tissue of a rhesus macaque inoculated with hepatitis C virus (HCV) has been analyzed for the presence of HCV RNA using the technique of in situ hybridization, both at light and electron microscopy levels. The animal was inoculated by the intrasplenic route using a HCV infected autogenic hepatocyte transplant. The serum sample used to infect the hepatocyte cells was characterized by polymerase chain reaction technique and shown to be positive for HCV RNA, genotype 3 with 10(7) RNA copies/ml. In situ hybridization was performed using a complementary negative strand probe made with the specific primer. We were able to detect and localize viral RNA in altered membranes of the rough endoplasmic reticulum of infected liver cells, showing evidence of virus replication in vivo.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/40270/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALDeboraFerguson_OrtrudMBarth_etal_IOC_2004.pdfDeboraFerguson_OrtrudMBarth_etal_IOC_2004.pdfapplication/pdf58294https://www.arca.fiocruz.br/bitstream/icict/40270/2/DeboraFerguson_OrtrudMBarth_etal_IOC_2004.pdf946df2c902194a84f748e8303c24d648MD52TEXTDeboraFerguson_OrtrudMBarth_etal_IOC_2004.pdf.txtDeboraFerguson_OrtrudMBarth_etal_IOC_2004.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv In situ hybridization of hepatitis C virus RNA in liver cells of an experimentally infected rhesus macaque
title In situ hybridization of hepatitis C virus RNA in liver cells of an experimentally infected rhesus macaque
spellingShingle In situ hybridization of hepatitis C virus RNA in liver cells of an experimentally infected rhesus macaque
Majerowicz, Selma
Vírus da Hepatite C
Hibridização In Situ
Macaca mulatta
In situ hybridization
Hepatitis C virus
Rhesus macaque
Macaca mulatta
title_short In situ hybridization of hepatitis C virus RNA in liver cells of an experimentally infected rhesus macaque
title_full In situ hybridization of hepatitis C virus RNA in liver cells of an experimentally infected rhesus macaque
title_fullStr In situ hybridization of hepatitis C virus RNA in liver cells of an experimentally infected rhesus macaque
title_full_unstemmed In situ hybridization of hepatitis C virus RNA in liver cells of an experimentally infected rhesus macaque
title_sort In situ hybridization of hepatitis C virus RNA in liver cells of an experimentally infected rhesus macaque
author Majerowicz, Selma
author_facet Majerowicz, Selma
Grief, Christopher
Ferguson, Debora
Airano, Renata C.
Baptista, Marcia L.
Pinto, Marcelo A.
Barth, Ortrud Monika
author_role author
author2 Grief, Christopher
Ferguson, Debora
Airano, Renata C.
Baptista, Marcia L.
Pinto, Marcelo A.
Barth, Ortrud Monika
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Majerowicz, Selma
Grief, Christopher
Ferguson, Debora
Airano, Renata C.
Baptista, Marcia L.
Pinto, Marcelo A.
Barth, Ortrud Monika
dc.subject.other.pt_BR.fl_str_mv Vírus da Hepatite C
Hibridização In Situ
Macaca mulatta
topic Vírus da Hepatite C
Hibridização In Situ
Macaca mulatta
In situ hybridization
Hepatitis C virus
Rhesus macaque
Macaca mulatta
dc.subject.en.pt_BR.fl_str_mv In situ hybridization
Hepatitis C virus
Rhesus macaque
dc.subject.decs.pt_BR.fl_str_mv Macaca mulatta
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.
publishDate 2004
dc.date.issued.fl_str_mv 2004
dc.date.accessioned.fl_str_mv 2020-03-10T12:43:38Z
dc.date.available.fl_str_mv 2020-03-10T12:43:38Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MAJEROWICZ, Selma et al. In situ hybridization of hepatitis C virus RNA in liver cells of an experimentally infected rhesus macaque. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 99, n. 6, p. 629-631, Oct. 2004.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/40270
dc.identifier.issn.pt_BR.fl_str_mv 0074-0206
dc.identifier.doi.none.fl_str_mv 10.1590/s0074-02762004000600017
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv MAJEROWICZ, Selma et al. In situ hybridization of hepatitis C virus RNA in liver cells of an experimentally infected rhesus macaque. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 99, n. 6, p. 629-631, Oct. 2004.
0074-0206
10.1590/s0074-02762004000600017
1678-8060
url https://www.arca.fiocruz.br/handle/icict/40270
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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