Germinal center architecture disturbance during Plasmodium berghei ANKA infection in CBA mice

Detalhes bibliográficos
Autor(a) principal: Carvalho, Leonardo José de Moura
Data de Publicação: 2007
Outros Autores: Cruz, Maria de Fátima Ferreira da, Daniel-Ribeiro, Cláudio Tadeu, Machado, Marcelo Pelajo, Lenzi, Henrique Leonel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/3741
Resumo: Acknowledgements to Belmira Santos from the Fiocruz Animal House for providing the CBA mice, and Bruno Eschenazi from the Laboratory of Production and Treatment of Images/IOC/Fiocruz for processing the images. This work was supported with funds of the Laboratory of Malaria Research and the Department of Pathology (POM).
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spelling Carvalho, Leonardo José de MouraCruz, Maria de Fátima Ferreira daDaniel-Ribeiro, Cláudio TadeuMachado, Marcelo PelajoLenzi, Henrique Leonel2012-01-19T15:04:24Z2012-01-19T15:04:24Z2007CARVALHO, L. J. et al. Germinal center architecture disturbance during Plasmodium berghei ANKA infection in CBA mice. Malaria Journal, v.16, n.6, p.59, May 2007.475-2875https://www.arca.fiocruz.br/handle/icict/374110.1186/1475-2875-6-59Acknowledgements to Belmira Santos from the Fiocruz Animal House for providing the CBA mice, and Bruno Eschenazi from the Laboratory of Production and Treatment of Images/IOC/Fiocruz for processing the images. This work was supported with funds of the Laboratory of Malaria Research and the Department of Pathology (POM).Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, BrasilBackground Immune responses to malaria blood stage infection are in general defective, with the need for long-term exposure to the parasite to achieve immunity, and with the development of immunopathology states such as cerebral malaria in many cases. One of the potential reasons for the difficulty in developing protective immunity is the poor development of memory responses. In this paper, the potential association of cellular reactivity in lymphoid organs (spleen, lymph nodes and Peyer's patches) with immunity and pathology was evaluated during Plasmodium berghei ANKA infection in CBA mice. Methods CBA mice were infected with 1 × 106 P. berghei ANKA-parasitized erythrocytes and killed on days 3, 6–8 and 10 of infection. The spleen, lymph nodes and Peyer's patches were collected, fixed in Carson's formalin, cut in 5 μm sections, mounted in glass slides, stained with Lennert's Giemsa and haematoxylin-eosin and analysed with bright-field microscopy. Results Early (day 3) strong activation of T cells in secondary lymphoid organs was observed and, on days 6–8 of infection, there was overwhelming activation of B cells, with loss of conventional germinal center architecture, intense centroblast activation, proliferation and apoptosis but little differentiation to centrocytes. In the spleen, the marginal zone disappeared and the limits between the disorganized germinal center and the red pulp were blurred. Intense plasmacytogenesis was observed in the T cell zone. Conclusion The observed alterations, especially the germinal center architecture disturbance (GCAD) with poor centrocyte differentiation, suggest that B cell responses during P. berghei ANKA infection in mice are defective, with potential impact on B cell memory responses.engBioMed CentralGerminal center architecture disturbance during Plasmodium berghei ANKA infection in CBA miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleMiceGerminal CenterB-LymphocytesImmunologyPlasmodium bergheiT-LymphocytesMalariaMaláriaImunologiaPlasmodium bergheiCentro GerminativoLinfócitos BLinfócitos TCamundongosinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALGerminal center architecture disturbance during Plasmodium.pdfGerminal center architecture disturbance during Plasmodium.pdfapplication/pdf1199963https://www.arca.fiocruz.br/bitstream/icict/3741/1/Germinal%20center%20architecture%20disturbance%20during%20Plasmodium.pdf5cc23473b5511718394ca72caa03f2abMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81648https://www.arca.fiocruz.br/bitstream/icict/3741/2/license.txte095249ac7cacefbfe39684dfe45e706MD52TEXTGerminal center architecture disturbance during Plasmodium.pdf.txtGerminal center architecture disturbance during Plasmodium.pdf.txtExtracted texttext/plain39758https://www.arca.fiocruz.br/bitstream/icict/3741/5/Germinal%20center%20architecture%20disturbance%20during%20Plasmodium.pdf.txt9c4efe5d213ef0eb0c39b620fc30f73fMD55THUMBNAILGerminal center architecture disturbance during Plasmodium.pdf.jpgGerminal center architecture disturbance during Plasmodium.pdf.jpgGenerated Thumbnailimage/jpeg2114https://www.arca.fiocruz.br/bitstream/icict/3741/4/Germinal%20center%20architecture%20disturbance%20during%20Plasmodium.pdf.jpg3a5e4a0fec9c627facb2293f789a4f2dMD54icict/37412020-07-17 16:12:26.082oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352020-07-17T19:12:26Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Germinal center architecture disturbance during Plasmodium berghei ANKA infection in CBA mice
title Germinal center architecture disturbance during Plasmodium berghei ANKA infection in CBA mice
spellingShingle Germinal center architecture disturbance during Plasmodium berghei ANKA infection in CBA mice
Carvalho, Leonardo José de Moura
Mice
Germinal Center
B-Lymphocytes
Immunology
Plasmodium berghei
T-Lymphocytes
Malaria
Malária
Imunologia
Plasmodium berghei
Centro Germinativo
Linfócitos B
Linfócitos T
Camundongos
title_short Germinal center architecture disturbance during Plasmodium berghei ANKA infection in CBA mice
title_full Germinal center architecture disturbance during Plasmodium berghei ANKA infection in CBA mice
title_fullStr Germinal center architecture disturbance during Plasmodium berghei ANKA infection in CBA mice
title_full_unstemmed Germinal center architecture disturbance during Plasmodium berghei ANKA infection in CBA mice
title_sort Germinal center architecture disturbance during Plasmodium berghei ANKA infection in CBA mice
author Carvalho, Leonardo José de Moura
author_facet Carvalho, Leonardo José de Moura
Cruz, Maria de Fátima Ferreira da
Daniel-Ribeiro, Cláudio Tadeu
Machado, Marcelo Pelajo
Lenzi, Henrique Leonel
author_role author
author2 Cruz, Maria de Fátima Ferreira da
Daniel-Ribeiro, Cláudio Tadeu
Machado, Marcelo Pelajo
Lenzi, Henrique Leonel
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Carvalho, Leonardo José de Moura
Cruz, Maria de Fátima Ferreira da
Daniel-Ribeiro, Cláudio Tadeu
Machado, Marcelo Pelajo
Lenzi, Henrique Leonel
dc.subject.en.pt_BR.fl_str_mv Mice
Germinal Center
B-Lymphocytes
Immunology
Plasmodium berghei
T-Lymphocytes
Malaria
topic Mice
Germinal Center
B-Lymphocytes
Immunology
Plasmodium berghei
T-Lymphocytes
Malaria
Malária
Imunologia
Plasmodium berghei
Centro Germinativo
Linfócitos B
Linfócitos T
Camundongos
dc.subject.decs.pt_BR.fl_str_mv Malária
Imunologia
Plasmodium berghei
Centro Germinativo
Linfócitos B
Linfócitos T
Camundongos
description Acknowledgements to Belmira Santos from the Fiocruz Animal House for providing the CBA mice, and Bruno Eschenazi from the Laboratory of Production and Treatment of Images/IOC/Fiocruz for processing the images. This work was supported with funds of the Laboratory of Malaria Research and the Department of Pathology (POM).
publishDate 2007
dc.date.issued.fl_str_mv 2007
dc.date.accessioned.fl_str_mv 2012-01-19T15:04:24Z
dc.date.available.fl_str_mv 2012-01-19T15:04:24Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CARVALHO, L. J. et al. Germinal center architecture disturbance during Plasmodium berghei ANKA infection in CBA mice. Malaria Journal, v.16, n.6, p.59, May 2007.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/3741
dc.identifier.issn.none.fl_str_mv 475-2875
dc.identifier.doi.none.fl_str_mv 10.1186/1475-2875-6-59
identifier_str_mv CARVALHO, L. J. et al. Germinal center architecture disturbance during Plasmodium berghei ANKA infection in CBA mice. Malaria Journal, v.16, n.6, p.59, May 2007.
475-2875
10.1186/1475-2875-6-59
url https://www.arca.fiocruz.br/handle/icict/3741
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dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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