Colorectal cancer DNA methylation patterns from patients in Manaus, Brazil
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2015 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/15494 |
Resumo: | CAPES, CNPq, Cancer Foundation (Oncobiologia Grant), and FAPEAM |
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Almeida, Fabiana Greyce OliveiraAquino, Priscila Ferreira deSouza, Afonso Duarte Leão deSouza, Antonia Queiroz Lima deVinhote, Sonia do CarmoMac-Cormick, Thaís MessiasSilva Marcelo Soares da MotaChalub, Sidney Raimundo SilvaFischer, Juliana de Saldanha da GamaCarvalho, Paulo CostaCarvalho, Maria da Gloria da Costa2016-08-26T16:31:19Z2016-08-26T16:31:19Z2015Almeida FGO, de Aquino PF, de Souza ADL, et al. Colorectal cancer DNA methylation patterns from patients in Manaus, Brazil. Biological Research, v. 48, n. 1, p. 50, 2015.0716-9760https://www.arca.fiocruz.br/handle/icict/1549410.1186/s40659-015-0042-7CAPES, CNPq, Cancer Foundation (Oncobiologia Grant), and FAPEAMFederal University of Amazonas. Institute of Exact Sciences. Laboratory of Chromatography and Mass Spectrometry. Manaus, AM, Brazil.Oswaldo Cruz Fundation. Instituto Leonidas e Maria Deane. Manaus, AM, Brazil.Amazon State University. High School of Health. Manaus, AM, Brazil.Federal University of Rio de Janeiro. Department of Pathology. Laboratory of Molecular Pathology. Rio de Janeiro, RJ, Brazil.Oncology Control Center Foundation of Amazon State. Department of Abdominal Surgery. Manaus, AM, Brazil.Oswaldo Cruz Fundation. Carlos Chagas Institute. Laboratory of Proteomics and Protein Engineering. Curitiba, PR, Brazil.Background DNA methylation is commonly linked with the silencing of the gene expression for many tumor suppressor genes. As such, determining DNA methylation patterns should aid, in times to come, in the diagnosis and personal treatment for various types of cancers. Here, we analyzed the methylation pattern from five colorectal cancer patients from the Amazon state in Brazil for four tumor suppressor genes, viz.: DAPK, CDH1, CDKN2A, and TIMP2 by employing a polymerase chain reaction (PCR) specific to methylation. Efforts in the study of colorectal cancer are fundamental as it is the third most of highest incidence in the world. Results Tumor biopsies were methylated in 1/5 (20 %), 2/5 (40 %), 4/5 (80 %), and 4/5 (80 %) for CDH1, CDKN2A, DAPK, and TIMP2 genes, respectively. The margin biopsies were methylated in 3/7 (43 %), 2/7 (28 %), 7/7 (100 %), and 6/7 (86 %) for CDH1, CDKN2A, DAPK, and TIMP2, respectively. Conclusions Our findings showed DAPK and TIMP2 to be methylated in most samples from both tumor tissues and adjacent non-neoplastic margins; thus presenting distinct methylation patterns. This emphasizes the importance of better understanding of the relation of these patterns with cancer in the context of different populations.porColorectal cancer DNA methylation patterns from patients in Manaus, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleMethylationColorectal cancerAmazonEpigeneticinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/15494/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALColorectal cancer DNA methyolation patterns from patients in Manaus, Brazil.pdfColorectal cancer DNA methyolation patterns from patients in Manaus, Brazil.pdfapplication/pdf1058445https://www.arca.fiocruz.br/bitstream/icict/15494/2/Colorectal%20cancer%20DNA%20methyolation%20patterns%20from%20patients%20in%20Manaus%2c%20Brazil.pdf309836b0a370d8ddc2ab281f81de6959MD52TEXTColorectal cancer DNA methyolation patterns from patients in Manaus, Brazil.pdf.txtColorectal cancer DNA methyolation patterns from patients in Manaus, Brazil.pdf.txtExtracted texttext/plain30624https://www.arca.fiocruz.br/bitstream/icict/15494/3/Colorectal%20cancer%20DNA%20methyolation%20patterns%20from%20patients%20in%20Manaus%2c%20Brazil.pdf.txtcea2fbefc356770637851cdf73ce7457MD53icict/154942019-10-31 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| dc.title.pt_BR.fl_str_mv |
Colorectal cancer DNA methylation patterns from patients in Manaus, Brazil |
| title |
Colorectal cancer DNA methylation patterns from patients in Manaus, Brazil |
| spellingShingle |
Colorectal cancer DNA methylation patterns from patients in Manaus, Brazil Almeida, Fabiana Greyce Oliveira Methylation Colorectal cancer Amazon Epigenetic |
| title_short |
Colorectal cancer DNA methylation patterns from patients in Manaus, Brazil |
| title_full |
Colorectal cancer DNA methylation patterns from patients in Manaus, Brazil |
| title_fullStr |
Colorectal cancer DNA methylation patterns from patients in Manaus, Brazil |
| title_full_unstemmed |
Colorectal cancer DNA methylation patterns from patients in Manaus, Brazil |
| title_sort |
Colorectal cancer DNA methylation patterns from patients in Manaus, Brazil |
| author |
Almeida, Fabiana Greyce Oliveira |
| author_facet |
Almeida, Fabiana Greyce Oliveira Aquino, Priscila Ferreira de Souza, Afonso Duarte Leão de Souza, Antonia Queiroz Lima de Vinhote, Sonia do Carmo Mac-Cormick, Thaís Messias Silva Marcelo Soares da Mota Chalub, Sidney Raimundo Silva Fischer, Juliana de Saldanha da Gama Carvalho, Paulo Costa Carvalho, Maria da Gloria da Costa |
| author_role |
author |
| author2 |
Aquino, Priscila Ferreira de Souza, Afonso Duarte Leão de Souza, Antonia Queiroz Lima de Vinhote, Sonia do Carmo Mac-Cormick, Thaís Messias Silva Marcelo Soares da Mota Chalub, Sidney Raimundo Silva Fischer, Juliana de Saldanha da Gama Carvalho, Paulo Costa Carvalho, Maria da Gloria da Costa |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Almeida, Fabiana Greyce Oliveira Aquino, Priscila Ferreira de Souza, Afonso Duarte Leão de Souza, Antonia Queiroz Lima de Vinhote, Sonia do Carmo Mac-Cormick, Thaís Messias Silva Marcelo Soares da Mota Chalub, Sidney Raimundo Silva Fischer, Juliana de Saldanha da Gama Carvalho, Paulo Costa Carvalho, Maria da Gloria da Costa |
| dc.subject.en.pt_BR.fl_str_mv |
Methylation Colorectal cancer Amazon Epigenetic |
| topic |
Methylation Colorectal cancer Amazon Epigenetic |
| description |
CAPES, CNPq, Cancer Foundation (Oncobiologia Grant), and FAPEAM |
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2015 |
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2015 |
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2016-08-26T16:31:19Z |
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2016-08-26T16:31:19Z |
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Almeida FGO, de Aquino PF, de Souza ADL, et al. Colorectal cancer DNA methylation patterns from patients in Manaus, Brazil. Biological Research, v. 48, n. 1, p. 50, 2015. |
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https://www.arca.fiocruz.br/handle/icict/15494 |
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0716-9760 |
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10.1186/s40659-015-0042-7 |
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Almeida FGO, de Aquino PF, de Souza ADL, et al. Colorectal cancer DNA methylation patterns from patients in Manaus, Brazil. Biological Research, v. 48, n. 1, p. 50, 2015. 0716-9760 10.1186/s40659-015-0042-7 |
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