Predicting the progress of colon cancer by DNA methylation markers of the p16 gene in feces - Evidence from an animal model

Detalhes bibliográficos
Autor(a) principal: Wu,Wen-Chih
Data de Publicação: 2013
Outros Autores: Hsu,Chih-Hsiung, Kuan,Jen-Chun, Hsieh,Jih-Fu, Sun,Chien-An, Yang,Tsan, Wu,Chang-Chieh, Chou,Yu-Ching
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300004
Resumo: A new noninvasive screening tool for colorectal neoplasia detects epigenetic alterations exhibited by gastrointestinal tumor cells shed into stool. There is insufficient existing data to determine temporal associations between colorectal cancer (CRC) progression and aberrant DNA methylation. To evaluate the feasibility of using fecal DNA methylation status to determine CRC progression, we collected stool samples from 14 male SD rats aged six weeks, and administered subcutaneous injections of either 1,2-dimethylhydrazine or saline weekly. p16 DNA methylation statuses in tumorous and normal colon tissue, and from stool samples were determined using methylation-specific PCR. Additionally, p16 methylation was detected in stool DNA from 85.7% of the CRC rats. The earliest change in p16 methylation status in the DMH-treated group stool samples occurred during week nine; repeatabilities were 57.1% in week 19 (p = 0.070) and 85.7% in week 34 (p = 0.005). A temporal correlation was evidenced between progression of CRC and p16 methylation status, as evidenced by DMH-induced rat feces. Using fecal DNA methylation status to determine colorectal tissue methylation status can reveal CRC progression. Our data suggests that p16 promoter methylation is a feasible epigenetic marker for the detection and may be useful for CRC screening.
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spelling Predicting the progress of colon cancer by DNA methylation markers of the p16 gene in feces - Evidence from an animal modelcolorectal cancerDNA methylationstool test for colorectal cancerA new noninvasive screening tool for colorectal neoplasia detects epigenetic alterations exhibited by gastrointestinal tumor cells shed into stool. There is insufficient existing data to determine temporal associations between colorectal cancer (CRC) progression and aberrant DNA methylation. To evaluate the feasibility of using fecal DNA methylation status to determine CRC progression, we collected stool samples from 14 male SD rats aged six weeks, and administered subcutaneous injections of either 1,2-dimethylhydrazine or saline weekly. p16 DNA methylation statuses in tumorous and normal colon tissue, and from stool samples were determined using methylation-specific PCR. Additionally, p16 methylation was detected in stool DNA from 85.7% of the CRC rats. The earliest change in p16 methylation status in the DMH-treated group stool samples occurred during week nine; repeatabilities were 57.1% in week 19 (p = 0.070) and 85.7% in week 34 (p = 0.005). A temporal correlation was evidenced between progression of CRC and p16 methylation status, as evidenced by DMH-induced rat feces. Using fecal DNA methylation status to determine colorectal tissue methylation status can reveal CRC progression. Our data suggests that p16 promoter methylation is a feasible epigenetic marker for the detection and may be useful for CRC screening.Sociedade Brasileira de Genética2013-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300004Genetics and Molecular Biology v.36 n.3 2013reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572013000300004info:eu-repo/semantics/openAccessWu,Wen-ChihHsu,Chih-HsiungKuan,Jen-ChunHsieh,Jih-FuSun,Chien-AnYang,TsanWu,Chang-ChiehChou,Yu-Chingeng2013-08-23T00:00:00Zoai:scielo:S1415-47572013000300004Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2013-08-23T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Predicting the progress of colon cancer by DNA methylation markers of the p16 gene in feces - Evidence from an animal model
title Predicting the progress of colon cancer by DNA methylation markers of the p16 gene in feces - Evidence from an animal model
spellingShingle Predicting the progress of colon cancer by DNA methylation markers of the p16 gene in feces - Evidence from an animal model
Wu,Wen-Chih
colorectal cancer
DNA methylation
stool test for colorectal cancer
title_short Predicting the progress of colon cancer by DNA methylation markers of the p16 gene in feces - Evidence from an animal model
title_full Predicting the progress of colon cancer by DNA methylation markers of the p16 gene in feces - Evidence from an animal model
title_fullStr Predicting the progress of colon cancer by DNA methylation markers of the p16 gene in feces - Evidence from an animal model
title_full_unstemmed Predicting the progress of colon cancer by DNA methylation markers of the p16 gene in feces - Evidence from an animal model
title_sort Predicting the progress of colon cancer by DNA methylation markers of the p16 gene in feces - Evidence from an animal model
author Wu,Wen-Chih
author_facet Wu,Wen-Chih
Hsu,Chih-Hsiung
Kuan,Jen-Chun
Hsieh,Jih-Fu
Sun,Chien-An
Yang,Tsan
Wu,Chang-Chieh
Chou,Yu-Ching
author_role author
author2 Hsu,Chih-Hsiung
Kuan,Jen-Chun
Hsieh,Jih-Fu
Sun,Chien-An
Yang,Tsan
Wu,Chang-Chieh
Chou,Yu-Ching
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Wu,Wen-Chih
Hsu,Chih-Hsiung
Kuan,Jen-Chun
Hsieh,Jih-Fu
Sun,Chien-An
Yang,Tsan
Wu,Chang-Chieh
Chou,Yu-Ching
dc.subject.por.fl_str_mv colorectal cancer
DNA methylation
stool test for colorectal cancer
topic colorectal cancer
DNA methylation
stool test for colorectal cancer
description A new noninvasive screening tool for colorectal neoplasia detects epigenetic alterations exhibited by gastrointestinal tumor cells shed into stool. There is insufficient existing data to determine temporal associations between colorectal cancer (CRC) progression and aberrant DNA methylation. To evaluate the feasibility of using fecal DNA methylation status to determine CRC progression, we collected stool samples from 14 male SD rats aged six weeks, and administered subcutaneous injections of either 1,2-dimethylhydrazine or saline weekly. p16 DNA methylation statuses in tumorous and normal colon tissue, and from stool samples were determined using methylation-specific PCR. Additionally, p16 methylation was detected in stool DNA from 85.7% of the CRC rats. The earliest change in p16 methylation status in the DMH-treated group stool samples occurred during week nine; repeatabilities were 57.1% in week 19 (p = 0.070) and 85.7% in week 34 (p = 0.005). A temporal correlation was evidenced between progression of CRC and p16 methylation status, as evidenced by DMH-induced rat feces. Using fecal DNA methylation status to determine colorectal tissue methylation status can reveal CRC progression. Our data suggests that p16 promoter methylation is a feasible epigenetic marker for the detection and may be useful for CRC screening.
publishDate 2013
dc.date.none.fl_str_mv 2013-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300004
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572013000300004
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.36 n.3 2013
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
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