Predicting the progress of colon cancer by DNA methylation markers of the p16 gene in feces - Evidence from an animal model
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300004 |
Resumo: | A new noninvasive screening tool for colorectal neoplasia detects epigenetic alterations exhibited by gastrointestinal tumor cells shed into stool. There is insufficient existing data to determine temporal associations between colorectal cancer (CRC) progression and aberrant DNA methylation. To evaluate the feasibility of using fecal DNA methylation status to determine CRC progression, we collected stool samples from 14 male SD rats aged six weeks, and administered subcutaneous injections of either 1,2-dimethylhydrazine or saline weekly. p16 DNA methylation statuses in tumorous and normal colon tissue, and from stool samples were determined using methylation-specific PCR. Additionally, p16 methylation was detected in stool DNA from 85.7% of the CRC rats. The earliest change in p16 methylation status in the DMH-treated group stool samples occurred during week nine; repeatabilities were 57.1% in week 19 (p = 0.070) and 85.7% in week 34 (p = 0.005). A temporal correlation was evidenced between progression of CRC and p16 methylation status, as evidenced by DMH-induced rat feces. Using fecal DNA methylation status to determine colorectal tissue methylation status can reveal CRC progression. Our data suggests that p16 promoter methylation is a feasible epigenetic marker for the detection and may be useful for CRC screening. |
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Predicting the progress of colon cancer by DNA methylation markers of the p16 gene in feces - Evidence from an animal modelcolorectal cancerDNA methylationstool test for colorectal cancerA new noninvasive screening tool for colorectal neoplasia detects epigenetic alterations exhibited by gastrointestinal tumor cells shed into stool. There is insufficient existing data to determine temporal associations between colorectal cancer (CRC) progression and aberrant DNA methylation. To evaluate the feasibility of using fecal DNA methylation status to determine CRC progression, we collected stool samples from 14 male SD rats aged six weeks, and administered subcutaneous injections of either 1,2-dimethylhydrazine or saline weekly. p16 DNA methylation statuses in tumorous and normal colon tissue, and from stool samples were determined using methylation-specific PCR. Additionally, p16 methylation was detected in stool DNA from 85.7% of the CRC rats. The earliest change in p16 methylation status in the DMH-treated group stool samples occurred during week nine; repeatabilities were 57.1% in week 19 (p = 0.070) and 85.7% in week 34 (p = 0.005). A temporal correlation was evidenced between progression of CRC and p16 methylation status, as evidenced by DMH-induced rat feces. Using fecal DNA methylation status to determine colorectal tissue methylation status can reveal CRC progression. Our data suggests that p16 promoter methylation is a feasible epigenetic marker for the detection and may be useful for CRC screening.Sociedade Brasileira de Genética2013-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300004Genetics and Molecular Biology v.36 n.3 2013reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572013000300004info:eu-repo/semantics/openAccessWu,Wen-ChihHsu,Chih-HsiungKuan,Jen-ChunHsieh,Jih-FuSun,Chien-AnYang,TsanWu,Chang-ChiehChou,Yu-Chingeng2013-08-23T00:00:00Zoai:scielo:S1415-47572013000300004Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2013-08-23T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Predicting the progress of colon cancer by DNA methylation markers of the p16 gene in feces - Evidence from an animal model |
title |
Predicting the progress of colon cancer by DNA methylation markers of the p16 gene in feces - Evidence from an animal model |
spellingShingle |
Predicting the progress of colon cancer by DNA methylation markers of the p16 gene in feces - Evidence from an animal model Wu,Wen-Chih colorectal cancer DNA methylation stool test for colorectal cancer |
title_short |
Predicting the progress of colon cancer by DNA methylation markers of the p16 gene in feces - Evidence from an animal model |
title_full |
Predicting the progress of colon cancer by DNA methylation markers of the p16 gene in feces - Evidence from an animal model |
title_fullStr |
Predicting the progress of colon cancer by DNA methylation markers of the p16 gene in feces - Evidence from an animal model |
title_full_unstemmed |
Predicting the progress of colon cancer by DNA methylation markers of the p16 gene in feces - Evidence from an animal model |
title_sort |
Predicting the progress of colon cancer by DNA methylation markers of the p16 gene in feces - Evidence from an animal model |
author |
Wu,Wen-Chih |
author_facet |
Wu,Wen-Chih Hsu,Chih-Hsiung Kuan,Jen-Chun Hsieh,Jih-Fu Sun,Chien-An Yang,Tsan Wu,Chang-Chieh Chou,Yu-Ching |
author_role |
author |
author2 |
Hsu,Chih-Hsiung Kuan,Jen-Chun Hsieh,Jih-Fu Sun,Chien-An Yang,Tsan Wu,Chang-Chieh Chou,Yu-Ching |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Wu,Wen-Chih Hsu,Chih-Hsiung Kuan,Jen-Chun Hsieh,Jih-Fu Sun,Chien-An Yang,Tsan Wu,Chang-Chieh Chou,Yu-Ching |
dc.subject.por.fl_str_mv |
colorectal cancer DNA methylation stool test for colorectal cancer |
topic |
colorectal cancer DNA methylation stool test for colorectal cancer |
description |
A new noninvasive screening tool for colorectal neoplasia detects epigenetic alterations exhibited by gastrointestinal tumor cells shed into stool. There is insufficient existing data to determine temporal associations between colorectal cancer (CRC) progression and aberrant DNA methylation. To evaluate the feasibility of using fecal DNA methylation status to determine CRC progression, we collected stool samples from 14 male SD rats aged six weeks, and administered subcutaneous injections of either 1,2-dimethylhydrazine or saline weekly. p16 DNA methylation statuses in tumorous and normal colon tissue, and from stool samples were determined using methylation-specific PCR. Additionally, p16 methylation was detected in stool DNA from 85.7% of the CRC rats. The earliest change in p16 methylation status in the DMH-treated group stool samples occurred during week nine; repeatabilities were 57.1% in week 19 (p = 0.070) and 85.7% in week 34 (p = 0.005). A temporal correlation was evidenced between progression of CRC and p16 methylation status, as evidenced by DMH-induced rat feces. Using fecal DNA methylation status to determine colorectal tissue methylation status can reveal CRC progression. Our data suggests that p16 promoter methylation is a feasible epigenetic marker for the detection and may be useful for CRC screening. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300004 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1415-47572013000300004 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.36 n.3 2013 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
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1752122385436246016 |