MLST reveals a clonal population structure for Cryptococcus neoformans molecular type VNI isolates from clinical sources in Amazonas, Northern-Brazil

Detalhes bibliográficos
Autor(a) principal: Rocha, Diego Fernando Silva
Data de Publicação: 2018
Outros Autores: Cruz, Katia Santana, Santos, Carla Silvana da Silva, Menescal, Lizandra Stephanny Fernandes, Silva Neto, João Ricardo da, Pinheiro, Silviane Bezerra, Silva, Lucyane Mendes, Trilles, Luciana, Souza, João Vicente Braga de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/33973
Resumo: Tropical Medicine Foundation Dr. Heitor Vieira Dourado. Medical Mycology Laboratory. Manaus, AM, Brazil / Environment and Health of National Research Institute of Amazonia. Coordination of Society. Mycology Laboratory. Manaus, AM, Brazil.
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spelling Rocha, Diego Fernando SilvaCruz, Katia SantanaSantos, Carla Silvana da SilvaMenescal, Lizandra Stephanny FernandesSilva Neto, João Ricardo daPinheiro, Silviane BezerraSilva, Lucyane MendesTrilles, LucianaSouza, João Vicente Braga de2019-07-11T12:16:19Z2019-07-11T12:16:19Z2018ROCHA, Diego Fernando Silva et al. MLST reveals a clonal population structure for Cryptococcus neoformans molecular type VNI isolates from clinical sources in Amazonas, Northern-Brazil. Plos One, p. 1-15, June 2018.1932-6203https://www.arca.fiocruz.br/handle/icict/3397310.1371/journal.pone.01978411932-6203engPublic Library of ScienceMLST reveals a clonal population structure for Cryptococcus neoformans molecular type VNI isolates from clinical sources in Amazonas, Northern-Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleTropical Medicine Foundation Dr. Heitor Vieira Dourado. Medical Mycology Laboratory. Manaus, AM, Brazil / Environment and Health of National Research Institute of Amazonia. Coordination of Society. Mycology Laboratory. Manaus, AM, Brazil.Tropical Medicine Foundation Dr. Heitor Vieira Dourado. Medical Mycology Laboratory. Manaus, AM, Brazil.Tropical Medicine Foundation Dr. Heitor Vieira Dourado. Medical Mycology Laboratory. Manaus, AM, Brazil.Tropical Medicine Foundation Dr. Heitor Vieira Dourado. Medical Mycology Laboratory. Manaus, AM, Brazil.Tropical Medicine Foundation Dr. Heitor Vieira Dourado. Medical Mycology Laboratory. Manaus, AM, Brazil.Environment and Health of National Research Institute of Amazonia. Coordination of Society. Mycology Laboratory. Manaus, AM, Brazil.Environment and Health of National Research Institute of Amazonia. Coordination of Society. Mycology Laboratory. Manaus, AM, Brazil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Environment and Health of National Research Institute of Amazonia. Coordination of Society. Mycology Laboratory. Manaus, AM, Brazil.Cryptococcosis is considered endemic in Amazonas state, occurring more frequently in individuals with AIDS, who are predominantly infected by Cryptococcus neoformans molecular type VNI. Infections by Cryptococcus gattii VGII predominate in immunocompetent hosts from the American continent and are associated with outbreaks in North America, particularly the subtypes VGIIa and VGIIb, which are also present in the Brazilian Amazon region. Despite few environmental studies, several aspects of the molecular epidemiology of this disease in Amazonas remain unclear, including the limited use of multilocus sequence typing (MLST) to evaluate the genetic population structure of clinical isolates, mainly C. neoformans. Therefore, we used MLST to identify the sequence types of 38 clinical isolates of C. neoformans VNI and C. gattii VGII and used phylogenetic analysis to evaluate their genetic relationship to global isolates. Records of 30 patients were analyzed to describe the current scenario of cryptococcosis in the region and their associations with the different subtypes. Broth microdilution was also performed to determine the susceptibility profile to the antifungals amphotericin B, fluconazole and itraconazole. MLST identified that patients with HIV (n = 26) were exclusively affected by VNI strains with ST93, and among the VGII strains (n = 4), three STs (ST5, ST172 and the new ST445) were identified. An in-hospital lethality of 54% was observed in the HIV group, and there were no significant differences in the clinical aspects of the disease between the HIV and non-HIV groups of patients. In addition, all isolates were susceptible to the antifungals tested. Therefore, in Amazonas state, VNI isolates are a genetically monotypic group, with ST93 being highly important in HIV individuals.Cryptococcus neoformansMLSTVNIAmazonasNorthern-Brazilinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83104https://www.arca.fiocruz.br/bitstream/icict/33973/1/license.txt79178e5f2a0eb066867a274556814938MD51ORIGINALve_Rocha_Diego_etal_INI_2018.pdfve_Rocha_Diego_etal_INI_2018.pdfapplication/pdf3157025https://www.arca.fiocruz.br/bitstream/icict/33973/2/ve_Rocha_Diego_etal_INI_2018.pdffb56bab5e5dd5ee5f9df2a494390a313MD52TEXTve_Rocha_Diego_etal_INI_2018.pdf.txtve_Rocha_Diego_etal_INI_2018.pdf.txtExtracted texttext/plain56180https://www.arca.fiocruz.br/bitstream/icict/33973/3/ve_Rocha_Diego_etal_INI_2018.pdf.txt807c9220a624d2d68b3e2a5e8100c1a0MD53icict/339732019-08-07 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dc.title.pt_BR.fl_str_mv MLST reveals a clonal population structure for Cryptococcus neoformans molecular type VNI isolates from clinical sources in Amazonas, Northern-Brazil
title MLST reveals a clonal population structure for Cryptococcus neoformans molecular type VNI isolates from clinical sources in Amazonas, Northern-Brazil
spellingShingle MLST reveals a clonal population structure for Cryptococcus neoformans molecular type VNI isolates from clinical sources in Amazonas, Northern-Brazil
Rocha, Diego Fernando Silva
Cryptococcus neoformans
MLST
VNI
Amazonas
Northern-Brazil
title_short MLST reveals a clonal population structure for Cryptococcus neoformans molecular type VNI isolates from clinical sources in Amazonas, Northern-Brazil
title_full MLST reveals a clonal population structure for Cryptococcus neoformans molecular type VNI isolates from clinical sources in Amazonas, Northern-Brazil
title_fullStr MLST reveals a clonal population structure for Cryptococcus neoformans molecular type VNI isolates from clinical sources in Amazonas, Northern-Brazil
title_full_unstemmed MLST reveals a clonal population structure for Cryptococcus neoformans molecular type VNI isolates from clinical sources in Amazonas, Northern-Brazil
title_sort MLST reveals a clonal population structure for Cryptococcus neoformans molecular type VNI isolates from clinical sources in Amazonas, Northern-Brazil
author Rocha, Diego Fernando Silva
author_facet Rocha, Diego Fernando Silva
Cruz, Katia Santana
Santos, Carla Silvana da Silva
Menescal, Lizandra Stephanny Fernandes
Silva Neto, João Ricardo da
Pinheiro, Silviane Bezerra
Silva, Lucyane Mendes
Trilles, Luciana
Souza, João Vicente Braga de
author_role author
author2 Cruz, Katia Santana
Santos, Carla Silvana da Silva
Menescal, Lizandra Stephanny Fernandes
Silva Neto, João Ricardo da
Pinheiro, Silviane Bezerra
Silva, Lucyane Mendes
Trilles, Luciana
Souza, João Vicente Braga de
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rocha, Diego Fernando Silva
Cruz, Katia Santana
Santos, Carla Silvana da Silva
Menescal, Lizandra Stephanny Fernandes
Silva Neto, João Ricardo da
Pinheiro, Silviane Bezerra
Silva, Lucyane Mendes
Trilles, Luciana
Souza, João Vicente Braga de
dc.subject.en.pt_BR.fl_str_mv Cryptococcus neoformans
MLST
VNI
Amazonas
Northern-Brazil
topic Cryptococcus neoformans
MLST
VNI
Amazonas
Northern-Brazil
description Tropical Medicine Foundation Dr. Heitor Vieira Dourado. Medical Mycology Laboratory. Manaus, AM, Brazil / Environment and Health of National Research Institute of Amazonia. Coordination of Society. Mycology Laboratory. Manaus, AM, Brazil.
publishDate 2018
dc.date.issued.fl_str_mv 2018
dc.date.accessioned.fl_str_mv 2019-07-11T12:16:19Z
dc.date.available.fl_str_mv 2019-07-11T12:16:19Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ROCHA, Diego Fernando Silva et al. MLST reveals a clonal population structure for Cryptococcus neoformans molecular type VNI isolates from clinical sources in Amazonas, Northern-Brazil. Plos One, p. 1-15, June 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/33973
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0197841
dc.identifier.eissn.none.fl_str_mv 1932-6203
identifier_str_mv ROCHA, Diego Fernando Silva et al. MLST reveals a clonal population structure for Cryptococcus neoformans molecular type VNI isolates from clinical sources in Amazonas, Northern-Brazil. Plos One, p. 1-15, June 2018.
1932-6203
10.1371/journal.pone.0197841
url https://www.arca.fiocruz.br/handle/icict/33973
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/33973/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/33973/2/ve_Rocha_Diego_etal_INI_2018.pdf
https://www.arca.fiocruz.br/bitstream/icict/33973/3/ve_Rocha_Diego_etal_INI_2018.pdf.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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