Expression signatures of DNA repair genes correlate with survival prognosis of astrocytoma patients

Detalhes bibliográficos
Autor(a) principal: Sousa, Juliana Ferreira de
Data de Publicação: 2017
Outros Autores: Torrieri, Raul, Serafim, Rodolfo Bortolozo, Di Cristofaro, Luis Fernando Macedo, Escanfella, Fábio Dalbon, Ribeiro, Rodrigo, Zanette, Dalila Lucíola, Larson, Maria Luisa Paçó, Silva, Wilson Araujo da, Tirapelli, Daniela Pretti da Cunha, Neder, Luciano, Carlotti, Carlos Gilberto, Valente, Valeria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/25652
Resumo: Zanette, Dalila Lucíola. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.
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spelling Sousa, Juliana Ferreira deTorrieri, RaulSerafim, Rodolfo BortolozoDi Cristofaro, Luis Fernando MacedoEscanfella, Fábio DalbonRibeiro, RodrigoZanette, Dalila LucíolaLarson, Maria Luisa PaçóSilva, Wilson Araujo daTirapelli, Daniela Pretti da CunhaNeder, LucianoCarlotti, Carlos GilbertoValente, Valeria2018-04-06T13:45:43Z2018-04-06T13:45:43Z2017SOUSA, J. F. et al. Expression signatures of DNA repair genes correlate with survival prognosis of astrocytoma patients1010-4283https://www.arca.fiocruz.br/handle/icict/2565210.1177/1010428317694552Zanette, Dalila Lucíola. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.Fundação de Amparo à Pesquisa do Estado de São Paulo (grant no. 2013/13465-1) and Programa de Apoio ao Desenvolvimento Científico (grant no. 204/2012) from Faculty of Pharmaceutical Sciences of Araraquara. Center for Cell-Based Therapy (CEPID/FAPESP; grant no. 2013/08135-2).University of São Paulo State. Faculty of Pharmaceutical Sciences of Araraquara. Department of Clinical Analysis. Araraquara, SP, Brazil / University of São Paulo. Ribeirão Preto Medical School. Department of Cellular and Molecular Biology. Ribeirão Preto, SP, BrazilUniversity of São Paulo. Ribeirão Preto Medical School. Center for Medical Genomics of the Clinical Hospital. Ribeirão Preto, SP, BrazilUniversity of São Paulo State. Faculty of Pharmaceutical Sciences of Araraquara. Department of Clinical Analysis. Araraquara, SP, Brazil / University of São Paulo. Ribeirão Preto Medical School. Department of Cellular and Molecular Biology. Ribeirão Preto, SP, BrazilUniversity of São Paulo State. Faculty of Pharmaceutical Sciences of Araraquara. Department of Clinical Analysis. Araraquara, SP, Brazil / University of São Paulo. Ribeirão Preto Medical School. Department of Cellular and Molecular Biology. Ribeirão Preto, SP, BrazilUniversity of São Paulo. Ribeirão Preto Medical School. Department of Cellular and Molecular Biology. Ribeirão Preto, SP, BrazilUniversity of São Paulo. Ribeirão Preto Medical School. Department of Cellular and Molecular Biology. Ribeirão Preto, SP, BrazilUniversity of São Paulo. Ribeirão Preto Medical School. Department of Genetics. Ribeirão Preto, SP, Brazil / Regional Blood Center of Ribeirão Preto. Center for Cell-Based Therapy. Ribeirão Preto, SP, Brazil / National Institute of Science and Technology in Stem cell and Cell Therapy. Ribeirão Preto, SP, BrazilUniversity of São Paulo. Ribeirão Preto Medical School. Department of Cellular and Molecular Biology. Ribeirão Preto, SP, BrazilUniversity of São Paulo. Ribeirão Preto Medical School. Department of Genetics. Ribeirão Preto, SP, Brazil / Regional Blood Center of Ribeirão Preto. Center for Cell-Based Therapy. Ribeirão Preto, SP, Brazil / National Institute of Science and Technology in Stem cell and Cell Therapy. Ribeirão Preto, SP, Brazil / University of São Paulo. Center for Integrative Systems Biology. Ribeirão Preto, SP, BrazilUniversity of São Paulo. Ribeirão Preto Medical School. Department of Surgery and Anatomy. Ribeirão Preto, SP, BrazilUniversity of São Paulo. Ribeirão Preto Medical School. Department of Pathology. Ribeirão Preto, SP, BrazilUniversity of São Paulo. Center for Integrative Systems Biology. Ribeirão Preto, SP, Brazil / University of São Paulo. Ribeirão Preto Medical School. Department of Pathology. Ribeirão Preto, SP, BrazilUniversity of São Paulo State. Faculty of Pharmaceutical Sciences of Araraquara. Department of Clinical Analysis. Araraquara, SP, Brazil / University of São Paulo. Ribeirão Preto Medical School. Department of Cellular and Molecular Biology. Ribeirão Preto, SP, Brazil / University of São Paulo. Center for Integrative Systems Biology. Ribeirão Preto, SP, BrazilAstrocytomas are the most common primary brain tumors. They are very resistant to therapies and usually progress rapidly to high-grade lesions. Here, we investigated the potential role of DNA repair genes in astrocytoma progression and resistance. To this aim, we performed a polymerase chain reaction array-based analysis focused on DNA repair genes and searched for correlations between expression patters and survival prognoses. We found 19 genes significantly altered. Combining these genes in all possible arrangements, we found 421 expression signatures strongly associated with poor survival. Importantly, five genes (DDB2, EXO1, NEIL3, BRCA2, and BRIP1) were independently correlated with worse prognoses, revealing single-gene signatures. Moreover, silencing of EXO1, which is remarkably overexpressed, promoted faster restoration of double-strand breaks, while NEIL3 knockdown, also highly overexpressed, caused an increment in DNA damage and cell death after irradiation of glioblastoma cells. These results disclose the importance of DNA repair pathways for the maintenance of genomic stability of high-grade astrocytomas and suggest that EXO1 and NEIL3 overexpression confers more efficiency for double-strand break repair and resistance to reactive oxygen species, respectively. Thereby, we highlight these two genes as potentially related with tumor aggressiveness and promising candidates as novel therapeutic targets.engSpringer VerlagReparo de DNAAstrocitomaGlioblastomaProgressão tumoralInstabilidade genômicaDNA repairAstrocytomaGlioblastomaTumor progressionGenomic instabilityExpression signatures of DNA repair genes correlate with survival prognosis of astrocytoma patientsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/25652/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSousa JF Expression signatures of DNA repair genes....pdfSousa JF Expression signatures of DNA repair 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dc.title.pt_BR.fl_str_mv Expression signatures of DNA repair genes correlate with survival prognosis of astrocytoma patients
title Expression signatures of DNA repair genes correlate with survival prognosis of astrocytoma patients
spellingShingle Expression signatures of DNA repair genes correlate with survival prognosis of astrocytoma patients
Sousa, Juliana Ferreira de
Reparo de DNA
Astrocitoma
Glioblastoma
Progressão tumoral
Instabilidade genômica
DNA repair
Astrocytoma
Glioblastoma
Tumor progression
Genomic instability
title_short Expression signatures of DNA repair genes correlate with survival prognosis of astrocytoma patients
title_full Expression signatures of DNA repair genes correlate with survival prognosis of astrocytoma patients
title_fullStr Expression signatures of DNA repair genes correlate with survival prognosis of astrocytoma patients
title_full_unstemmed Expression signatures of DNA repair genes correlate with survival prognosis of astrocytoma patients
title_sort Expression signatures of DNA repair genes correlate with survival prognosis of astrocytoma patients
author Sousa, Juliana Ferreira de
author_facet Sousa, Juliana Ferreira de
Torrieri, Raul
Serafim, Rodolfo Bortolozo
Di Cristofaro, Luis Fernando Macedo
Escanfella, Fábio Dalbon
Ribeiro, Rodrigo
Zanette, Dalila Lucíola
Larson, Maria Luisa Paçó
Silva, Wilson Araujo da
Tirapelli, Daniela Pretti da Cunha
Neder, Luciano
Carlotti, Carlos Gilberto
Valente, Valeria
author_role author
author2 Torrieri, Raul
Serafim, Rodolfo Bortolozo
Di Cristofaro, Luis Fernando Macedo
Escanfella, Fábio Dalbon
Ribeiro, Rodrigo
Zanette, Dalila Lucíola
Larson, Maria Luisa Paçó
Silva, Wilson Araujo da
Tirapelli, Daniela Pretti da Cunha
Neder, Luciano
Carlotti, Carlos Gilberto
Valente, Valeria
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Sousa, Juliana Ferreira de
Torrieri, Raul
Serafim, Rodolfo Bortolozo
Di Cristofaro, Luis Fernando Macedo
Escanfella, Fábio Dalbon
Ribeiro, Rodrigo
Zanette, Dalila Lucíola
Larson, Maria Luisa Paçó
Silva, Wilson Araujo da
Tirapelli, Daniela Pretti da Cunha
Neder, Luciano
Carlotti, Carlos Gilberto
Valente, Valeria
dc.subject.other.pt_BR.fl_str_mv Reparo de DNA
Astrocitoma
Glioblastoma
Progressão tumoral
Instabilidade genômica
topic Reparo de DNA
Astrocitoma
Glioblastoma
Progressão tumoral
Instabilidade genômica
DNA repair
Astrocytoma
Glioblastoma
Tumor progression
Genomic instability
dc.subject.en.pt_BR.fl_str_mv DNA repair
Astrocytoma
Glioblastoma
Tumor progression
Genomic instability
description Zanette, Dalila Lucíola. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2018-04-06T13:45:43Z
dc.date.available.fl_str_mv 2018-04-06T13:45:43Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SOUSA, J. F. et al. Expression signatures of DNA repair genes correlate with survival prognosis of astrocytoma patients
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/25652
dc.identifier.issn.pt_BR.fl_str_mv 1010-4283
dc.identifier.doi.none.fl_str_mv 10.1177/1010428317694552
identifier_str_mv SOUSA, J. F. et al. Expression signatures of DNA repair genes correlate with survival prognosis of astrocytoma patients
1010-4283
10.1177/1010428317694552
url https://www.arca.fiocruz.br/handle/icict/25652
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Verlag
publisher.none.fl_str_mv Springer Verlag
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/25652/1/license.txt
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https://www.arca.fiocruz.br/bitstream/icict/25652/3/Sousa%20JF%20Expression%20signatures%20of%20DNA%20repair%20genes....pdf.txt
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