The Schistosomula tegument antigen as a potential candidate for the erly serological diagnosis of esquistossomoseISTOSOMIASIS MANSONI

Detalhes bibliográficos
Autor(a) principal: Queiroz, Rafaella Fortini Grenfell e
Data de Publicação: 2013
Outros Autores: Martins, Watson, Moraes, Vanessa Silva, Araujo, Neusa, Oliveira, Edward José ds, Toscano, Cristina Fonseca, Coelho, Paulo Marcos Zech
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1590/S0036-46652013000200002
Texto Completo: https://www.arca.fiocruz.br/handle/icict/50544
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brazil
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spelling Queiroz, Rafaella Fortini Grenfell eMartins, WatsonMoraes, Vanessa SilvaAraujo, NeusaOliveira, Edward José dsToscano, Cristina FonsecaCoelho, Paulo Marcos Zech2021-12-28T15:38:22Z2021-12-28T15:38:22Z2013QUEIROZ, Rafaella Fortini Grenfell e et al. Antígenos de tegumento de esquistossômulos são candidatos em potencial para o diagnóstico de fase aguda da esquistossomose mansoni. Rev. Inst. Med. Trop. Sao Paulo, v. 55, n. 2, p. 75-78, 2013.1678-9946https://www.arca.fiocruz.br/handle/icict/5054410.1590/S0036-46652013000200002engInstituto de Medicina Tropical de São PauloThe Schistosomula tegument antigen as a potential candidate for the erly serological diagnosis of esquistossomoseISTOSOMIASIS MANSONIinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Pesquisa Clínicas. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, BrazilIf Schistosoma mansoni infection could be detected in its early stages, especially before the egg deposition in the host tissues, the development of severe pathologic lesions could be efficiently prevented. We therefore developed an indirect enzyme-linked immunosorbent assay based on the detection of specific IgG against schistosomula antigens (ELISA-SmTeg). The assay was applied in sera samples from non-infected and infected mice collected seven and 15 days post-infection. The results were compared to the number of adult worms obtained by perfusion of the murine hepatic system 50 days post-infection. The sensitivity and specificity of the ELISA-SmTeg were 100% (p = 0.0032 and 0.0048 respectively for seven and 15 days of infection) with a cutoff value of 0.15 (p = 0.0002). Our findings show a novel low-cost serological assay using antigens which are easy to obtain, which was able to detect all the infected mice as early as seven days post-infectionExperimental approachAcute schistosomiasisDiagnosisImmunological assaySchistosomula antigensinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/50544/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALTHE SCHISTOSOMULA TEGUMENT ANTIGEN.pdfTHE SCHISTOSOMULA TEGUMENT ANTIGEN.pdfapplication/pdf186718https://www.arca.fiocruz.br/bitstream/icict/50544/2/THE%20SCHISTOSOMULA%20TEGUMENT%20ANTIGEN.pdf7b86dfa4b4efc15129b0008cbb6c663aMD52icict/505442021-12-28 12:38:22.354oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352021-12-28T15:38:22Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv The Schistosomula tegument antigen as a potential candidate for the erly serological diagnosis of esquistossomoseISTOSOMIASIS MANSONI
title The Schistosomula tegument antigen as a potential candidate for the erly serological diagnosis of esquistossomoseISTOSOMIASIS MANSONI
spellingShingle The Schistosomula tegument antigen as a potential candidate for the erly serological diagnosis of esquistossomoseISTOSOMIASIS MANSONI
Queiroz, Rafaella Fortini Grenfell e
Experimental approach
Acute schistosomiasis
Diagnosis
Immunological assay
Schistosomula antigens
title_short The Schistosomula tegument antigen as a potential candidate for the erly serological diagnosis of esquistossomoseISTOSOMIASIS MANSONI
title_full The Schistosomula tegument antigen as a potential candidate for the erly serological diagnosis of esquistossomoseISTOSOMIASIS MANSONI
title_fullStr The Schistosomula tegument antigen as a potential candidate for the erly serological diagnosis of esquistossomoseISTOSOMIASIS MANSONI
title_full_unstemmed The Schistosomula tegument antigen as a potential candidate for the erly serological diagnosis of esquistossomoseISTOSOMIASIS MANSONI
title_sort The Schistosomula tegument antigen as a potential candidate for the erly serological diagnosis of esquistossomoseISTOSOMIASIS MANSONI
author Queiroz, Rafaella Fortini Grenfell e
author_facet Queiroz, Rafaella Fortini Grenfell e
Martins, Watson
Moraes, Vanessa Silva
Araujo, Neusa
Oliveira, Edward José ds
Toscano, Cristina Fonseca
Coelho, Paulo Marcos Zech
author_role author
author2 Martins, Watson
Moraes, Vanessa Silva
Araujo, Neusa
Oliveira, Edward José ds
Toscano, Cristina Fonseca
Coelho, Paulo Marcos Zech
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Queiroz, Rafaella Fortini Grenfell e
Martins, Watson
Moraes, Vanessa Silva
Araujo, Neusa
Oliveira, Edward José ds
Toscano, Cristina Fonseca
Coelho, Paulo Marcos Zech
dc.subject.en.pt_BR.fl_str_mv Experimental approach
Acute schistosomiasis
Diagnosis
Immunological assay
Schistosomula antigens
topic Experimental approach
Acute schistosomiasis
Diagnosis
Immunological assay
Schistosomula antigens
description Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brazil
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2021-12-28T15:38:22Z
dc.date.available.fl_str_mv 2021-12-28T15:38:22Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv QUEIROZ, Rafaella Fortini Grenfell e et al. Antígenos de tegumento de esquistossômulos são candidatos em potencial para o diagnóstico de fase aguda da esquistossomose mansoni. Rev. Inst. Med. Trop. Sao Paulo, v. 55, n. 2, p. 75-78, 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/50544
dc.identifier.issn.pt_BR.fl_str_mv 1678-9946
dc.identifier.doi.none.fl_str_mv 10.1590/S0036-46652013000200002
identifier_str_mv QUEIROZ, Rafaella Fortini Grenfell e et al. Antígenos de tegumento de esquistossômulos são candidatos em potencial para o diagnóstico de fase aguda da esquistossomose mansoni. Rev. Inst. Med. Trop. Sao Paulo, v. 55, n. 2, p. 75-78, 2013.
1678-9946
10.1590/S0036-46652013000200002
url https://www.arca.fiocruz.br/handle/icict/50544
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dc.publisher.none.fl_str_mv Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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