Molecular characterization of influenza viruses collected from young children in Uberlandia, Brazil - from 2001 to 2010
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2015 |
| Outros Autores: | , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/13850 |
Resumo: | Universidade Federal de Uberlândia (UFU). Instituto de Ciências Biomédicas. Laboratório de Virologia. Uberlândia, MG, Brasil. |
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Oliveira, Thelma Fátima de Mattos SilvaYokosawa, JonnyMotta, Fernando CoutoSiqueira, Marilda Agudo Mendonça Teixeira deSilveira, Hélio Lopes daQueiróz, Divina Aparecida Oliveira2016-04-18T16:05:32Z2016-04-18T16:05:32Z2015OLIVEIRA, Thelma Fátima de Mattos Silva; et al. Molecular characterization of influenza viruses collected from young children in Uberlandia, Brazil - from 2001 to 2010. BMC Infectious Diseases, v.15:71, 9p, 2015.1471-2334https://www.arca.fiocruz.br/handle/icict/1385010.1186/s12879-015-0817-zengBioMed CentralVírus da InfluenzaCriançasProteína HNNeuraminidaseReação em Cadeia da PolimeraseUberlândiaInfluenza virusChildrenRT-PCRSequencingHemagglutininNeuraminidaseMolecular characterization of influenza viruses collected from young children in Uberlandia, Brazil - from 2001 to 2010info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Uberlândia (UFU). Instituto de Ciências Biomédicas. Laboratório de Virologia. Uberlândia, MG, Brasil.Universidade Federal de Uberlândia (UFU). Instituto de Ciências Biomédicas. Laboratório de Virologia. Uberlândia, MG, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratórios. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratórios. Rio de Janeiro, RJ, Brasil.Universidade Federal de Uberlândia (UFU). Faculdade de Medicina. Uberlândia, MG, Brasil.Universidade Federal de Uberlândia (UFU). Instituto de Ciências Biomédicas. Laboratório de Virologia. Uberlândia, MG, Brasil.Background: Influenza remains a major health problem due to the seasonal epidemics that occur every year caused by the emergence of new influenza virus strains. Hemagglutinin (HA) and neuraminidase (NA) glycoproteins are under selective pressure and subjected to frequent changes by antigenic drift. Therefore, our main objective was to investigate the influenza cases in Uberlândia city, Midwestern Brazil, in order to monitor the appearance of new viral strains, despite the availability of a prophylactic vaccine. Methods: Nasopharyngeal samples were collected from 605 children less than five years of age presenting with acute respiratory disease and tested by immunofluorescence assay (IFA) for detection of adenovirus, respiratory syncytial virus, parainfluenza virus types 1, 2, and 3 and influenza virus types A and B. A reverse transcription-PCR (RT-PCR) for influenza viruses A and B was carried out to amplify partial segments of the HA and NA genes. The nucleotide sequences were analyzed and compared with sequences of the virus strains of the vaccine available in the same year of sample collection. Results: Forty samples (6.6%) were tested positive for influenza virus by IFA and RT-PCR, with 39 samples containing virus of type A and one of type B. By RT-PCR, the type A viruses were further characterized in subtypes H3N2, H1N2 and H1N1 (41.0%, 17.9%, and 2.6%, respectively). Deduced amino acid sequence analysis of the partial hemagglutinin sequence compared to sequences from vaccine strains, revealed that all strains found in Uberlândia had variations in the antigenic sites. The sequences of the receptor binding sites were preserved, although substitutions with similar amino acids were observed in few cases. The neuraminidase sequences did not show significant changes. All the H3 isolates detected in the 2001-2003 period had drifted from vaccine strain, unlike the isolates of the 2004-2007 period. Conclusions: These results suggest that the seasonal influenza vaccine effectiveness could be reduced because of A H3N2 variants that circulated in 2001-2003 years. Thus, an early monitoring of variants circulating in the country or in a region may provide important information about the probable efficacy of the vaccine that will be administered in an influenza season.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/13850/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALmarilda2_siqueira_etal_IOC_2015.pdfmarilda2_siqueira_etal_IOC_2015.pdfapplication/pdf469385https://www.arca.fiocruz.br/bitstream/icict/13850/2/marilda2_siqueira_etal_IOC_2015.pdf073d8213a1c0800966464156fabb2e73MD52TEXTmarilda2_siqueira_etal_IOC_2015.pdf.txtmarilda2_siqueira_etal_IOC_2015.pdf.txtExtracted texttext/plain50747https://www.arca.fiocruz.br/bitstream/icict/13850/3/marilda2_siqueira_etal_IOC_2015.pdf.txtf22fbf6d0a7b583f1ed29b66ad8d1d9dMD53icict/138502022-03-23 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| dc.title.pt_BR.fl_str_mv |
Molecular characterization of influenza viruses collected from young children in Uberlandia, Brazil - from 2001 to 2010 |
| title |
Molecular characterization of influenza viruses collected from young children in Uberlandia, Brazil - from 2001 to 2010 |
| spellingShingle |
Molecular characterization of influenza viruses collected from young children in Uberlandia, Brazil - from 2001 to 2010 Oliveira, Thelma Fátima de Mattos Silva Vírus da Influenza Crianças Proteína HN Neuraminidase Reação em Cadeia da Polimerase Uberlândia Influenza virus Children RT-PCR Sequencing Hemagglutinin Neuraminidase |
| title_short |
Molecular characterization of influenza viruses collected from young children in Uberlandia, Brazil - from 2001 to 2010 |
| title_full |
Molecular characterization of influenza viruses collected from young children in Uberlandia, Brazil - from 2001 to 2010 |
| title_fullStr |
Molecular characterization of influenza viruses collected from young children in Uberlandia, Brazil - from 2001 to 2010 |
| title_full_unstemmed |
Molecular characterization of influenza viruses collected from young children in Uberlandia, Brazil - from 2001 to 2010 |
| title_sort |
Molecular characterization of influenza viruses collected from young children in Uberlandia, Brazil - from 2001 to 2010 |
| author |
Oliveira, Thelma Fátima de Mattos Silva |
| author_facet |
Oliveira, Thelma Fátima de Mattos Silva Yokosawa, Jonny Motta, Fernando Couto Siqueira, Marilda Agudo Mendonça Teixeira de Silveira, Hélio Lopes da Queiróz, Divina Aparecida Oliveira |
| author_role |
author |
| author2 |
Yokosawa, Jonny Motta, Fernando Couto Siqueira, Marilda Agudo Mendonça Teixeira de Silveira, Hélio Lopes da Queiróz, Divina Aparecida Oliveira |
| author2_role |
author author author author author |
| dc.contributor.author.fl_str_mv |
Oliveira, Thelma Fátima de Mattos Silva Yokosawa, Jonny Motta, Fernando Couto Siqueira, Marilda Agudo Mendonça Teixeira de Silveira, Hélio Lopes da Queiróz, Divina Aparecida Oliveira |
| dc.subject.other.pt_BR.fl_str_mv |
Vírus da Influenza Crianças Proteína HN Neuraminidase Reação em Cadeia da Polimerase Uberlândia |
| topic |
Vírus da Influenza Crianças Proteína HN Neuraminidase Reação em Cadeia da Polimerase Uberlândia Influenza virus Children RT-PCR Sequencing Hemagglutinin Neuraminidase |
| dc.subject.en.pt_BR.fl_str_mv |
Influenza virus Children RT-PCR Sequencing Hemagglutinin Neuraminidase |
| description |
Universidade Federal de Uberlândia (UFU). Instituto de Ciências Biomédicas. Laboratório de Virologia. Uberlândia, MG, Brasil. |
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2015 |
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2015 |
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2016-04-18T16:05:32Z |
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2016-04-18T16:05:32Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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OLIVEIRA, Thelma Fátima de Mattos Silva; et al. Molecular characterization of influenza viruses collected from young children in Uberlandia, Brazil - from 2001 to 2010. BMC Infectious Diseases, v.15:71, 9p, 2015. |
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https://www.arca.fiocruz.br/handle/icict/13850 |
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1471-2334 |
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10.1186/s12879-015-0817-z |
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OLIVEIRA, Thelma Fátima de Mattos Silva; et al. Molecular characterization of influenza viruses collected from young children in Uberlandia, Brazil - from 2001 to 2010. BMC Infectious Diseases, v.15:71, 9p, 2015. 1471-2334 10.1186/s12879-015-0817-z |
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