Evolution of equine influenza viruses (H3N8) during a Brazilian outbreak, 2015

Detalhes bibliográficos
Autor(a) principal: Favaro,Patricia Filippsen
Data de Publicação: 2018
Outros Autores: Fernandes,Wilson Roberto, Reischak,Dilmara, Brandão,Paulo Eduardo, Silva,Sheila Oliveira de Souza, Richtzenhain,Leonardo José
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200336
Resumo: Abstract Equine influenza is one of the major respiratory infectious diseases in horses. An equine influenza virus outbreak was identified in vaccinated and unvaccinated horses in a veterinary school hospital in São Paulo, SP, Brazil, in September 2015. The twelve equine influenza viruses isolated belonged to Florida Clade 1. The hemagglutinin and neuraminidase amino acid sequences were compared with the recent isolates from North and South America and the World Organisation for Animal Health recommended Florida Clade 1 vaccine strain. The hemagglutinin amino acid sequences had nine substitutions, compared with the vaccine strain. Two of them were in antigenic site A (A138S and G142R), one in antigenic site E (R62K) and another not in antigenic site (K304E). The four substitutions changed the hydrophobicity of hemagglutinin. Three distinct genetic variants were identified during the outbreak. Eleven variants were found in four quasispecies, which suggests the equine influenza virus evolved during the outbreak. The use of an out of date vaccine strain or updated vaccines without the production of protective antibody titers might be the major contributing factors on virus dissemination during this outbreak.
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spelling Evolution of equine influenza viruses (H3N8) during a Brazilian outbreak, 2015Equine influenzaH3N8HemagglutininNeuraminidaseEvolutionary analysisAbstract Equine influenza is one of the major respiratory infectious diseases in horses. An equine influenza virus outbreak was identified in vaccinated and unvaccinated horses in a veterinary school hospital in São Paulo, SP, Brazil, in September 2015. The twelve equine influenza viruses isolated belonged to Florida Clade 1. The hemagglutinin and neuraminidase amino acid sequences were compared with the recent isolates from North and South America and the World Organisation for Animal Health recommended Florida Clade 1 vaccine strain. The hemagglutinin amino acid sequences had nine substitutions, compared with the vaccine strain. Two of them were in antigenic site A (A138S and G142R), one in antigenic site E (R62K) and another not in antigenic site (K304E). The four substitutions changed the hydrophobicity of hemagglutinin. Three distinct genetic variants were identified during the outbreak. Eleven variants were found in four quasispecies, which suggests the equine influenza virus evolved during the outbreak. The use of an out of date vaccine strain or updated vaccines without the production of protective antibody titers might be the major contributing factors on virus dissemination during this outbreak.Sociedade Brasileira de Microbiologia2018-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200336Brazilian Journal of Microbiology v.49 n.2 2018reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1016/j.bjm.2017.07.003info:eu-repo/semantics/openAccessFavaro,Patricia FilippsenFernandes,Wilson RobertoReischak,DilmaraBrandão,Paulo EduardoSilva,Sheila Oliveira de SouzaRichtzenhain,Leonardo Joséeng2018-04-17T00:00:00Zoai:scielo:S1517-83822018000200336Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2018-04-17T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Evolution of equine influenza viruses (H3N8) during a Brazilian outbreak, 2015
title Evolution of equine influenza viruses (H3N8) during a Brazilian outbreak, 2015
spellingShingle Evolution of equine influenza viruses (H3N8) during a Brazilian outbreak, 2015
Favaro,Patricia Filippsen
Equine influenza
H3N8
Hemagglutinin
Neuraminidase
Evolutionary analysis
title_short Evolution of equine influenza viruses (H3N8) during a Brazilian outbreak, 2015
title_full Evolution of equine influenza viruses (H3N8) during a Brazilian outbreak, 2015
title_fullStr Evolution of equine influenza viruses (H3N8) during a Brazilian outbreak, 2015
title_full_unstemmed Evolution of equine influenza viruses (H3N8) during a Brazilian outbreak, 2015
title_sort Evolution of equine influenza viruses (H3N8) during a Brazilian outbreak, 2015
author Favaro,Patricia Filippsen
author_facet Favaro,Patricia Filippsen
Fernandes,Wilson Roberto
Reischak,Dilmara
Brandão,Paulo Eduardo
Silva,Sheila Oliveira de Souza
Richtzenhain,Leonardo José
author_role author
author2 Fernandes,Wilson Roberto
Reischak,Dilmara
Brandão,Paulo Eduardo
Silva,Sheila Oliveira de Souza
Richtzenhain,Leonardo José
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Favaro,Patricia Filippsen
Fernandes,Wilson Roberto
Reischak,Dilmara
Brandão,Paulo Eduardo
Silva,Sheila Oliveira de Souza
Richtzenhain,Leonardo José
dc.subject.por.fl_str_mv Equine influenza
H3N8
Hemagglutinin
Neuraminidase
Evolutionary analysis
topic Equine influenza
H3N8
Hemagglutinin
Neuraminidase
Evolutionary analysis
description Abstract Equine influenza is one of the major respiratory infectious diseases in horses. An equine influenza virus outbreak was identified in vaccinated and unvaccinated horses in a veterinary school hospital in São Paulo, SP, Brazil, in September 2015. The twelve equine influenza viruses isolated belonged to Florida Clade 1. The hemagglutinin and neuraminidase amino acid sequences were compared with the recent isolates from North and South America and the World Organisation for Animal Health recommended Florida Clade 1 vaccine strain. The hemagglutinin amino acid sequences had nine substitutions, compared with the vaccine strain. Two of them were in antigenic site A (A138S and G142R), one in antigenic site E (R62K) and another not in antigenic site (K304E). The four substitutions changed the hydrophobicity of hemagglutinin. Three distinct genetic variants were identified during the outbreak. Eleven variants were found in four quasispecies, which suggests the equine influenza virus evolved during the outbreak. The use of an out of date vaccine strain or updated vaccines without the production of protective antibody titers might be the major contributing factors on virus dissemination during this outbreak.
publishDate 2018
dc.date.none.fl_str_mv 2018-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200336
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200336
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1016/j.bjm.2017.07.003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.49 n.2 2018
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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