Small interference ITGA6 gene targeting in the human thymic epithelium differentially regulates the expression of immunological synapse-related genes

Detalhes bibliográficos
Autor(a) principal: Golbert, Daiane Cristina F.
Data de Publicação: 2017
Outros Autores: Santana-Van-Vliet, Eliane, Ribeiro-Alves, Marcelo, Fonsêca, Marbella Maria B. da, Lepletier, Ailin, Mendes-da-Cruz, Daniella A., Loss, Guilherme, Cotta-de-Almeida, Vinícius, Vasconcelos, Ana Tereza R., Savino, Wilson
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/25612
Resumo: 2018-05-31
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spelling Golbert, Daiane Cristina F.Santana-Van-Vliet, ElianeRibeiro-Alves, MarceloFonsêca, Marbella Maria B. daLepletier, AilinMendes-da-Cruz, Daniella A.Loss, GuilhermeCotta-de-Almeida, ViníciusVasconcelos, Ana Tereza R.Savino, Wilson2018-04-04T15:17:12Z2018-04-04T15:17:12Z2017GOLBERT, Daiane Cristina F. et al. Small interference ITGA6 gene targeting in the human thymic epithelium differentially regulates the expression of immunological synapse-related genes. Cell Adhesion & Migration, v. 11, p. 1-28, May. 2017.1933-6918https://www.arca.fiocruz.br/handle/icict/2561210.1080/19336918.2017.1327513engTaylor & FrancisSmall interference ITGA6 gene targeting in the human thymic epithelium differentially regulates the expression of immunological synapse-related genesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2018-05-31Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil / Laboratório Nacional de Computação Científica. Laboratório de Bioinformática. Petrópolis, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, BrasilNuffield Department of Clinical Medicine. Structural Genomics Consortium. University of Oxford. Oxford, England.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Laboratório Nacional de Computação Científica. Laboratório de Bioinformática. Petropolis, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Laboratório Nacional de Computação Científica. Laboratório de Bioinformática. Petrópolis, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.The thymus supports differentiation of T cell precursors. This process requires relocation of developing thymocytes throughout multiple microenvironments of the organ, mainly with thymic epithelial cells (TEC), which control intrathymic T cell differentiation influencing the formation and maintenance of the immunological synapse. In addition to the proteins of the major histocompatibility complex (MHC), this structure is supported by several adhesion molecules. During the process of thymopoiesis, we previously showed that laminin-mediated interactions are involved in the entrance of T-cell precursors into the thymus, as well as migration of differentiating thymocytes within the organ. Using small interference RNA strategy, we knocked-down the ITGA6 gene (which encodes the CD49f integrin α-chain) in cultured human TEC, generating a decrease in the expression of the corresponding CD49f subunit, in addition to modulation in several other genes related to cell adhesion and migration. Thymocyte adhesion to TEC was significantly impaired, comprising both immature and mature thymocyte subsets. Moreover, we found a modulation of the MHC, with a decrease in membrane expression of HLA-ABC, in contrast with increase in the expression of HLA-DR. Interestingly, the knockdown of the B2M gene (encoding the β-2 microglobulin of the HLA-ABC complex) increased CD49f expression levels, thus unraveling the existence of a cross-talk event in the reciprocal control of CD49f and HLA-ABC. Our data suggest that the expression levels of CD49f may be relevant in the general control of MHC expression by TEC and consequently the corresponding synapse with developing thymocytes mediated by the T-cell receptor.Thymic epithelial cellsIntegrin alpha6RNA interferenceHLA-ABCHLA-DRExtracellular matrixIntegrin alpha6RNA interferenceHLA-DR antigensExtracellular matrixinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv Small interference ITGA6 gene targeting in the human thymic epithelium differentially regulates the expression of immunological synapse-related genes
title Small interference ITGA6 gene targeting in the human thymic epithelium differentially regulates the expression of immunological synapse-related genes
spellingShingle Small interference ITGA6 gene targeting in the human thymic epithelium differentially regulates the expression of immunological synapse-related genes
Golbert, Daiane Cristina F.
Thymic epithelial cells
Integrin alpha6
RNA interference
HLA-ABC
HLA-DR
Extracellular matrix
Integrin alpha6
RNA interference
HLA-DR antigens
Extracellular matrix
title_short Small interference ITGA6 gene targeting in the human thymic epithelium differentially regulates the expression of immunological synapse-related genes
title_full Small interference ITGA6 gene targeting in the human thymic epithelium differentially regulates the expression of immunological synapse-related genes
title_fullStr Small interference ITGA6 gene targeting in the human thymic epithelium differentially regulates the expression of immunological synapse-related genes
title_full_unstemmed Small interference ITGA6 gene targeting in the human thymic epithelium differentially regulates the expression of immunological synapse-related genes
title_sort Small interference ITGA6 gene targeting in the human thymic epithelium differentially regulates the expression of immunological synapse-related genes
author Golbert, Daiane Cristina F.
author_facet Golbert, Daiane Cristina F.
Santana-Van-Vliet, Eliane
Ribeiro-Alves, Marcelo
Fonsêca, Marbella Maria B. da
Lepletier, Ailin
Mendes-da-Cruz, Daniella A.
Loss, Guilherme
Cotta-de-Almeida, Vinícius
Vasconcelos, Ana Tereza R.
Savino, Wilson
author_role author
author2 Santana-Van-Vliet, Eliane
Ribeiro-Alves, Marcelo
Fonsêca, Marbella Maria B. da
Lepletier, Ailin
Mendes-da-Cruz, Daniella A.
Loss, Guilherme
Cotta-de-Almeida, Vinícius
Vasconcelos, Ana Tereza R.
Savino, Wilson
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Golbert, Daiane Cristina F.
Santana-Van-Vliet, Eliane
Ribeiro-Alves, Marcelo
Fonsêca, Marbella Maria B. da
Lepletier, Ailin
Mendes-da-Cruz, Daniella A.
Loss, Guilherme
Cotta-de-Almeida, Vinícius
Vasconcelos, Ana Tereza R.
Savino, Wilson
dc.subject.en.pt_BR.fl_str_mv Thymic epithelial cells
Integrin alpha6
RNA interference
HLA-ABC
HLA-DR
Extracellular matrix
topic Thymic epithelial cells
Integrin alpha6
RNA interference
HLA-ABC
HLA-DR
Extracellular matrix
Integrin alpha6
RNA interference
HLA-DR antigens
Extracellular matrix
dc.subject.decs.pt_BR.fl_str_mv Integrin alpha6
RNA interference
HLA-DR antigens
Extracellular matrix
description 2018-05-31
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2018-04-04T15:17:12Z
dc.date.available.fl_str_mv 2018-04-04T15:17:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GOLBERT, Daiane Cristina F. et al. Small interference ITGA6 gene targeting in the human thymic epithelium differentially regulates the expression of immunological synapse-related genes. Cell Adhesion & Migration, v. 11, p. 1-28, May. 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/25612
dc.identifier.issn.pt_BR.fl_str_mv 1933-6918
dc.identifier.doi.none.fl_str_mv 10.1080/19336918.2017.1327513
identifier_str_mv GOLBERT, Daiane Cristina F. et al. Small interference ITGA6 gene targeting in the human thymic epithelium differentially regulates the expression of immunological synapse-related genes. Cell Adhesion & Migration, v. 11, p. 1-28, May. 2017.
1933-6918
10.1080/19336918.2017.1327513
url https://www.arca.fiocruz.br/handle/icict/25612
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/25612/1/license.txt
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https://www.arca.fiocruz.br/bitstream/icict/25612/3/ve_Daiane_Golbert_etal_INI_Lapclin_2017.pdf.txt
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