The major circulating immunosuppressive activity in American visceral leishmaniasis patients is associated with a high-molecular weight fraction and is not mediated by IgG, IgG immune complexes or lipoproteins

Detalhes bibliográficos
Autor(a) principal: Soares, Neci Matos
Data de Publicação: 2006
Outros Autores: Ferraz, T. P. L, Nascimento, Eliane Góes, Carvalho Filho, Edgar Marcelino, Pontes-de-Carvalho, Lain Carlos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8305
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Salvador, BA, Brasil
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spelling Soares, Neci MatosFerraz, T. P. LNascimento, Eliane GóesCarvalho Filho, Edgar MarcelinoPontes-de-Carvalho, Lain Carlos2014-09-04T17:40:44Z2014-09-04T17:40:44Z2006SOARES, N. M. et al. The major circulating immunosuppressive activity in American visceral leishmaniasis patients is associated with a high-molecular weight fraction and is not mediated by IgG, IgG immune complexes or lipoproteins. Microbial Pathogenesis, v. 40, n. 6, p. 254-260, 2006.0882-4010https://www.arca.fiocruz.br/handle/icict/830510.1016/j.micpath.2006.02.005engElsevier LtdThe major circulating immunosuppressive activity in American visceral leishmaniasis patients is associated with a high-molecular weight fraction and is not mediated by IgG, IgG immune complexes or lipoproteinsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Salvador, BA, BrasilSecretaria da Saúde do Estado da Bahia. 13a DIRES. Jequié, BA, BrasilUniversidade Federal da Bahia. Hospital Universitário Professor Edgar Santos. Serviço de Imunologia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, BrasilOpportunistic infections, due to disease-related immunosuppression, constitute the major cause of death in American visceral leishmaniasis (AVL). Sera from these patients (AVL sera) non-specifically inhibit the in vitro proliferative response of normal human lymphocytes to lectins or antigens. In the present work, the mediation of this inhibition by IgG, immune complexes and low- or very low-density lipoproteins was studied. AVL serum fractions containing proteins with the molecular weight of IgG, and IgG, purified from AVL sera by anion exchange chromatography, did not suppress the lymphoproliferation. Most of the suppressive activity of AVL sera was associated with a fraction containing molecules with molecular weights above 430 kDa. This would be compatible with it being due to immune complexes and/or lipoproteins, and not to soluble IL-2 receptors as reported previously. However, neither of the two possibilities seem to be the case, as (1) depletion of immune complexes by protein-A followed by protein-G chromatographies did not affect the serum suppressive activity, (2) no correlation between immune complex contents and suppressive activities in individual sera was observed, and (3) plasma lipoproteins (VLDL and LDL), purified from AVL patients and from healthy individuals, had the same degree of immunosuppressive activity.LeishmaniaLymphocyteImmunosuppressionIgGImmune complexesLipoproteinsComplexo Antígeno-Anticorpo/imunologiaImunoglobulina G/imunologiaLeishmaniose Visceral/imunologiaLipoproteínas LDL/sangueLipoproteínas VLDL/sangueAnimaisConcanavalina A/farmacologiaHumanosHospedeiro ImunocomprometidoLeishmania infantumLeishmaniose Visceral/sangueLeucócitos Mononucleares/efeitos de drogasAtivação Linfocitária/efeitos de drogasinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8305/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALSoares NM The major circulating......pdfSoares NM The major circulating......pdfapplication/pdf209490https://www.arca.fiocruz.br/bitstream/icict/8305/2/Soares%20NM%20The%20major%20circulating......pdf1c9f558436a30e44e571c4b9c07b4093MD52TEXTSoares NM The major circulating......pdf.txtSoares NM The major circulating......pdf.txtExtracted texttext/plain31272https://www.arca.fiocruz.br/bitstream/icict/8305/3/Soares%20NM%20The%20major%20circulating......pdf.txt3533553af6402d45b9754925075d361fMD53icict/83052023-03-15 14:33:21.231oai:www.arca.fiocruz.br:icict/8305TElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTy1FWENMVVNJVkEKCkFvIGNvbmNvcmRhciBlIGFjZWl0YXIgZXN0YSBsaWNlbsOnYSB2b2PDqiAoYXV0b3Igb3UgZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzKToKCmEpIERlY2xhcmEgcXVlIGNvbmhlY2UgYSBwb2zDrXRpY2EgZGUgY29weXJpZ2h0IGRhIGVkaXRvcmEgZG8gc2V1IGRvY3VtZW50by4KCmIpIERlY2xhcmEgcXVlIGNvbmhlY2UgZSBhY2VpdGEgYXMgRGlyZXRyaXplcyBwYXJhIG8gUmVwb3NpdMOzcmlvIEluc3RpdHVjaW9uYWwgZGEgRnVuZGHDp8OjbyBPc3dhbGRvIENydXogKEZJT0NSVVopLgoKYykgQ29uY2VkZSDDoCBGSU9DUlVaIG8gZGlyZWl0byBuw6NvLWV4Y2x1c2l2byBkZSBhcnF1aXZhciwgcmVwcm9kdXppciwgY29udmVydGVyIChjb21vIGRlZmluaWRvIGEgc2VndWlyKSwgY29tdW5pY2FyCiAKZS9vdSBkaXN0cmlidWlyIG5vIFJlcG9zaXTDs3JpbyBkYSBGSU9DUlVaLCBvIGRvY3VtZW50byBlbnRyZWd1ZSAoaW5jbHVpbmRvIG8gcmVzdW1vL2Fic3RyYWN0KSBlbSBmb3JtYXRvIGRpZ2l0YWwgb3UgCgpwb3IgcXVhbHF1ZXIgb3V0cm8gbWVpby4KCmQpIERlY2xhcmEgcXVlIGF1dG9yaXphIGEgRklPQ1JVWiBhIGFycXVpdmFyIG1haXMgZGUgdW1hIGPDs3BpYSBkZXN0ZSBkb2N1bWVudG8gZSBjb252ZXJ0w6otbG8sIHNlbSBhbHRlcmFyIG8gc2V1IGNvbnRlw7pkbywgCgpwYXJhIHF1YWxxdWVyIGZvcm1hdG8gZGUgYXJxdWl2bywgbWVpbyBvdSBzdXBvcnRlLCBwYXJhIGVmZWl0b3MgZGUgc2VndXJhbsOnYSwgcHJlc2VydmHDp8OjbyAoYmFja3VwKSBlIGFjZXNzby4KCmUpIERlY2xhcmEgcXVlIG8gZG9jdW1lbnRvIHN1Ym1ldGlkbyDDqSBvIHNldSB0cmFiYWxobyBvcmlnaW5hbCwgZSBxdWUgZGV0w6ltIG8gZGlyZWl0byBkZSBjb25jZWRlciBhIHRlcmNlaXJvcyBvcyBkaXJlaXRvcyAKCmNvbnRpZG9zIG5lc3RhIGxpY2Vuw6dhLiBEZWNsYXJhIHRhbWLDqW0gcXVlIGEgZW50cmVnYSBkbyBkb2N1bWVudG8gbsOjbyBpbmZyaW5nZSBvcyBkaXJlaXRvcyBkZSBxdWFscXVlciBvdXRyYSBwZXNzb2Egb3UgZW50aWRhZGUuCgpmKSBEZWNsYXJhIHF1ZSwgbm8gY2FzbyBkbyBkb2N1bWVudG8gc3VibWV0aWRvIGNvbnRlciBtYXRlcmlhbCBkbyBxdWFsIG7Do28gZGV0w6ltIG9zIGRpcmVpdG9zIGRlIGF1dG9yLCBvYnRldmUgYSBhdXRvcml6YcOnw6NvIAoKaXJyZXN0cml0YSBkbyByZXNwZWN0aXZvIGRldGVudG9yIGRlc3NlcyBkaXJlaXRvcywgcGFyYSBjZWRlciBhIEZJT0NSVVogb3MgZGlyZWl0b3MgcmVxdWVyaWRvcyBwb3IgZXN0YSBMaWNlbsOnYSBlIGF1dG9yaXphciBhIAoKdXRpbGl6w6EtbG9zIGxlZ2FsbWVudGUuIERlY2xhcmEgdGFtYsOpbSBxdWUgZXNzZSBtYXRlcmlhbCBjdWpvcyBkaXJlaXRvcyBzw6NvIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIGlkZW50aWZpY2FkbyBlIHJlY29uaGVjaWRvIAoKbm8gdGV4dG8gb3UgY29udGXDumRvIGRvIGRvY3VtZW50byBlbnRyZWd1ZS4KCmcpIFNFIE8gRE9DVU1FTlRPIEVOVFJFR1VFIMOJIEJBU0VBRE8gRU0gVFJBQkFMSE8gRklOQU5DSUFETyBPVSBBUE9JQURPIFBPUiBPVVRSQSBJTlNUSVRVScOHw4NPIFFVRSBOw4NPIEEgRklPQ1JVWiwgREVDTEFSQSBRVUUgQ1VNUFJJVSAKClFVQUlTUVVFUiBPQlJJR0HDh8OVRVMgRVhJR0lEQVMgUEVMTyBSRVNQRUNUSVZPIENPTlRSQVRPIE9VIEFDT1JETy4gQSBGSU9DUlVaIGlkZW50aWZpY2Fyw6EgY2xhcmFtZW50ZSBvKHMpIG5vbWUocykgZG8ocykgYXV0b3IoZXMpIGRvcyAKCmRpcmVpdG9zIGRvIGRvY3VtZW50byBlbnRyZWd1ZSBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIHBhcmEgYWzDqW0gZG8gcHJldmlzdG8gbmEgYWzDrW5lYSBjKS4KRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:21Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv The major circulating immunosuppressive activity in American visceral leishmaniasis patients is associated with a high-molecular weight fraction and is not mediated by IgG, IgG immune complexes or lipoproteins
title The major circulating immunosuppressive activity in American visceral leishmaniasis patients is associated with a high-molecular weight fraction and is not mediated by IgG, IgG immune complexes or lipoproteins
spellingShingle The major circulating immunosuppressive activity in American visceral leishmaniasis patients is associated with a high-molecular weight fraction and is not mediated by IgG, IgG immune complexes or lipoproteins
Soares, Neci Matos
Leishmania
Lymphocyte
Immunosuppression
IgG
Immune complexes
Lipoproteins
Complexo Antígeno-Anticorpo/imunologia
Imunoglobulina G/imunologia
Leishmaniose Visceral/imunologia
Lipoproteínas LDL/sangue
Lipoproteínas VLDL/sangue
Animais
Concanavalina A/farmacologia
Humanos
Hospedeiro Imunocomprometido
Leishmania infantum
Leishmaniose Visceral/sangue
Leucócitos Mononucleares/efeitos de drogas
Ativação Linfocitária/efeitos de drogas
title_short The major circulating immunosuppressive activity in American visceral leishmaniasis patients is associated with a high-molecular weight fraction and is not mediated by IgG, IgG immune complexes or lipoproteins
title_full The major circulating immunosuppressive activity in American visceral leishmaniasis patients is associated with a high-molecular weight fraction and is not mediated by IgG, IgG immune complexes or lipoproteins
title_fullStr The major circulating immunosuppressive activity in American visceral leishmaniasis patients is associated with a high-molecular weight fraction and is not mediated by IgG, IgG immune complexes or lipoproteins
title_full_unstemmed The major circulating immunosuppressive activity in American visceral leishmaniasis patients is associated with a high-molecular weight fraction and is not mediated by IgG, IgG immune complexes or lipoproteins
title_sort The major circulating immunosuppressive activity in American visceral leishmaniasis patients is associated with a high-molecular weight fraction and is not mediated by IgG, IgG immune complexes or lipoproteins
author Soares, Neci Matos
author_facet Soares, Neci Matos
Ferraz, T. P. L
Nascimento, Eliane Góes
Carvalho Filho, Edgar Marcelino
Pontes-de-Carvalho, Lain Carlos
author_role author
author2 Ferraz, T. P. L
Nascimento, Eliane Góes
Carvalho Filho, Edgar Marcelino
Pontes-de-Carvalho, Lain Carlos
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Soares, Neci Matos
Ferraz, T. P. L
Nascimento, Eliane Góes
Carvalho Filho, Edgar Marcelino
Pontes-de-Carvalho, Lain Carlos
dc.subject.en.pt_BR.fl_str_mv Leishmania
Lymphocyte
Immunosuppression
IgG
Immune complexes
Lipoproteins
topic Leishmania
Lymphocyte
Immunosuppression
IgG
Immune complexes
Lipoproteins
Complexo Antígeno-Anticorpo/imunologia
Imunoglobulina G/imunologia
Leishmaniose Visceral/imunologia
Lipoproteínas LDL/sangue
Lipoproteínas VLDL/sangue
Animais
Concanavalina A/farmacologia
Humanos
Hospedeiro Imunocomprometido
Leishmania infantum
Leishmaniose Visceral/sangue
Leucócitos Mononucleares/efeitos de drogas
Ativação Linfocitária/efeitos de drogas
dc.subject.decs.pt_BR.fl_str_mv Complexo Antígeno-Anticorpo/imunologia
Imunoglobulina G/imunologia
Leishmaniose Visceral/imunologia
Lipoproteínas LDL/sangue
Lipoproteínas VLDL/sangue
Animais
Concanavalina A/farmacologia
Humanos
Hospedeiro Imunocomprometido
Leishmania infantum
Leishmaniose Visceral/sangue
Leucócitos Mononucleares/efeitos de drogas
Ativação Linfocitária/efeitos de drogas
description Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Salvador, BA, Brasil
publishDate 2006
dc.date.issued.fl_str_mv 2006
dc.date.accessioned.fl_str_mv 2014-09-04T17:40:44Z
dc.date.available.fl_str_mv 2014-09-04T17:40:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SOARES, N. M. et al. The major circulating immunosuppressive activity in American visceral leishmaniasis patients is associated with a high-molecular weight fraction and is not mediated by IgG, IgG immune complexes or lipoproteins. Microbial Pathogenesis, v. 40, n. 6, p. 254-260, 2006.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8305
dc.identifier.issn.none.fl_str_mv 0882-4010
dc.identifier.doi.none.fl_str_mv 10.1016/j.micpath.2006.02.005
identifier_str_mv SOARES, N. M. et al. The major circulating immunosuppressive activity in American visceral leishmaniasis patients is associated with a high-molecular weight fraction and is not mediated by IgG, IgG immune complexes or lipoproteins. Microbial Pathogenesis, v. 40, n. 6, p. 254-260, 2006.
0882-4010
10.1016/j.micpath.2006.02.005
url https://www.arca.fiocruz.br/handle/icict/8305
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier Ltd
publisher.none.fl_str_mv Elsevier Ltd
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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instacron_str FIOCRUZ
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