Meta-transcriptome Profiling of the Human-Leishmania braziliensis Cutaneous Lesion

Detalhes bibliográficos
Autor(a) principal: Christensen, Stephen M
Data de Publicação: 2016
Outros Autores: Dillon, Laura A L, Carvalho, Lucas Pedreira de, Passos, Sara Timoteo, Novais, Fernanda Oliveira, Hughitt, V Keith, Beiting, Daniel P, Carvalho Filho, Edgar Marcelino, Scott, Phillip, El-Sayed, Najib M, Mosser, David M
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/18368
Resumo: Carvalho Filho, E. M. Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.
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spelling Christensen, Stephen MDillon, Laura A LCarvalho, Lucas Pedreira dePassos, Sara TimoteoNovais, Fernanda OliveiraHughitt, V KeithBeiting, Daniel PCarvalho Filho, Edgar MarcelinoScott, PhillipEl-Sayed, Najib MMosser, David M2017-04-11T13:21:02Z2017-04-11T13:21:02Z2016CHRISTENSEN, S. M. et al. Meta-transcriptome Profiling of the Human-Leishmania braziliensis Cutaneous Lesion. PLoS Neglected Tropical Diseases, v. 10, n. 9, p. e0004992, 2016.1935-2727https://www.arca.fiocruz.br/handle/icict/1836810.1371/journal.pntd.0004992Carvalho Filho, E. M. Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.National Institutes of Health NIAID (AI094773) and NIGMS (GM102589), and na International Collaboration for Infectious Disease Research (ICIDR) grant (U01-AUI088650).University of Maryland. Department of Cell Biology and Molecular Genetics. Maryland, USAUniversity of Maryland. Department of Cell Biology and Molecular Genetics. Maryland, USA / University of Maryland. Center for Bioinformatics and Computational Biology. Maryland, USAUniversidade Federal da Bahia. Salvador, BA, BrasilUniversidade Federal da Bahia. Salvador, BA, BrasilThe University of Pennsylvania. School of Veterinary Medicine. Department of Pathobiology. Philadelphia, Pennsylvania, USAUniversity of Maryland. Department of Cell Biology and Molecular Genetics. Maryland, USA / University of Maryland. Center for Bioinformatics and Computational Biology. Maryland, USAThe University of Pennsylvania. School of Veterinary Medicine. Department of Pathobiology. Philadelphia, Pennsylvania, USAUniversidade Federal da Bahia. Salvador, BA, BrasilThe University of Pennsylvania. School of Veterinary Medicine. Department of Pathobiology. Philadelphia, Pennsylvania, USAUniversity of Maryland. Department of Cell Biology and Molecular Genetics. Maryland, USA / University of Maryland. Center for Bioinformatics and Computational Biology. Maryland, USAUniversity of Maryland. Department of Cell Biology and Molecular Genetics. Maryland, USAHost and parasite gene expression in skin biopsies from Leishmania braziliensis-infected patients were simultaneously analyzed using high throughput RNA-sequencing. Biopsies were taken from 8 patients with early cutaneous leishmaniasis and 17 patients with late cutaneous leishmaniasis. Although parasite DNA was found in all patient lesions at the time of biopsy, the patients could be stratified into two groups: one lacking detectable parasite transcripts (PTNeg) in lesions, and another in which parasite transcripts were readily detected (PTPos). These groups exhibited substantial differences in host responses to infection. PTPos biopsies contained an unexpected increase in B lymphocyte-specific and immunoglobulin transcripts in the lesions, and an upregulation of immune inhibitory molecules. Biopsies without detectable parasite transcripts showed decreased evidence for B cell activation, but increased expression of antimicrobial genes and genes encoding skin barrier functions. The composition and abundance of L. braziliensis transcripts in PTPos lesions were surprisingly conserved among all six patients, with minimal meaningful differences between lesions from patients with early and late cutaneous leishmaniasis. The most abundant parasite transcripts expressed in lesions were distinct from transcripts expressed in vitro in human macrophage cultures infected with L. amazonensis or L. major. Therefore in vitro gene expression in macrophage monolayers may not be a strong predictor of gene expression in lesions. Some of the most highly expressed in vivo transcripts encoded amastin-like proteins, hypothetical genes, putative parasite virulence factors, as well as histones and tubulin. In summary, RNA sequencing allowed us to simultaneously analyze human and L. braziliensis transcriptomes in lesions of infected patients, and identify unexpected differences in host immune responses which correlated with active transcription of parasite genes.engPublic Library of ScienceLeishmania braziliensisLeishmaniose cutâneaPeleHumanoBiópsiaDNAInfecçãoLeishmania braziliensisLeishmaniose cutâneaSkinHumansBiopsiesDNAInfectionMeta-transcriptome Profiling of the Human-Leishmania braziliensis Cutaneous Lesioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/18368/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALChristensen SM Meta-transctriptome....pdfChristensen SM 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dc.title.pt_BR.fl_str_mv Meta-transcriptome Profiling of the Human-Leishmania braziliensis Cutaneous Lesion
title Meta-transcriptome Profiling of the Human-Leishmania braziliensis Cutaneous Lesion
spellingShingle Meta-transcriptome Profiling of the Human-Leishmania braziliensis Cutaneous Lesion
Christensen, Stephen M
Leishmania braziliensis
Leishmaniose cutânea
Pele
Humano
Biópsia
DNA
Infecção
Leishmania braziliensis
Leishmaniose cutânea
Skin
Humans
Biopsies
DNA
Infection
title_short Meta-transcriptome Profiling of the Human-Leishmania braziliensis Cutaneous Lesion
title_full Meta-transcriptome Profiling of the Human-Leishmania braziliensis Cutaneous Lesion
title_fullStr Meta-transcriptome Profiling of the Human-Leishmania braziliensis Cutaneous Lesion
title_full_unstemmed Meta-transcriptome Profiling of the Human-Leishmania braziliensis Cutaneous Lesion
title_sort Meta-transcriptome Profiling of the Human-Leishmania braziliensis Cutaneous Lesion
author Christensen, Stephen M
author_facet Christensen, Stephen M
Dillon, Laura A L
Carvalho, Lucas Pedreira de
Passos, Sara Timoteo
Novais, Fernanda Oliveira
Hughitt, V Keith
Beiting, Daniel P
Carvalho Filho, Edgar Marcelino
Scott, Phillip
El-Sayed, Najib M
Mosser, David M
author_role author
author2 Dillon, Laura A L
Carvalho, Lucas Pedreira de
Passos, Sara Timoteo
Novais, Fernanda Oliveira
Hughitt, V Keith
Beiting, Daniel P
Carvalho Filho, Edgar Marcelino
Scott, Phillip
El-Sayed, Najib M
Mosser, David M
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Christensen, Stephen M
Dillon, Laura A L
Carvalho, Lucas Pedreira de
Passos, Sara Timoteo
Novais, Fernanda Oliveira
Hughitt, V Keith
Beiting, Daniel P
Carvalho Filho, Edgar Marcelino
Scott, Phillip
El-Sayed, Najib M
Mosser, David M
dc.subject.other.pt_BR.fl_str_mv Leishmania braziliensis
Leishmaniose cutânea
Pele
Humano
Biópsia
DNA
Infecção
topic Leishmania braziliensis
Leishmaniose cutânea
Pele
Humano
Biópsia
DNA
Infecção
Leishmania braziliensis
Leishmaniose cutânea
Skin
Humans
Biopsies
DNA
Infection
dc.subject.en.pt_BR.fl_str_mv Leishmania braziliensis
Leishmaniose cutânea
Skin
Humans
Biopsies
DNA
Infection
description Carvalho Filho, E. M. Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2017-04-11T13:21:02Z
dc.date.available.fl_str_mv 2017-04-11T13:21:02Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CHRISTENSEN, S. M. et al. Meta-transcriptome Profiling of the Human-Leishmania braziliensis Cutaneous Lesion. PLoS Neglected Tropical Diseases, v. 10, n. 9, p. e0004992, 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/18368
dc.identifier.issn.pt_BR.fl_str_mv 1935-2727
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0004992
identifier_str_mv CHRISTENSEN, S. M. et al. Meta-transcriptome Profiling of the Human-Leishmania braziliensis Cutaneous Lesion. PLoS Neglected Tropical Diseases, v. 10, n. 9, p. e0004992, 2016.
1935-2727
10.1371/journal.pntd.0004992
url https://www.arca.fiocruz.br/handle/icict/18368
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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