Genotyping and drug resistance patterns of Mycobacterium tuberculosis strains observed in a tuberculosis high-burden municipality in Northeast, Brazil
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2013 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/11579 |
Resumo: | Universidade Federal do Ceará. Escola de Medicina. Departamento de Patologia, Fortaleza, CE, Brasil. |
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Luiz, Roberta dos Santos SilvaSuffys, PhillipBarroso, Elizabeth ClaraKerr, Lígía Regina Franco SansigoloDuarte, Cynthia RomarizFreitas, Max Victor CariocaMota, Rosa Maria SalaniFrota, Cristiane Cunha2015-08-19T13:49:35Z2015-08-19T13:49:35Z2013LUIZ, Roberta dos Santos Silva; et al. Genotyping and drug resistance patterns of Mycobacterium tuberculosis strains observed in a tuberculosis high-burden municipality in Northeast, Brazil. Braz J Infect Dis, v.17, n.3, p.338-345, June 2013.1413-8670https://www.arca.fiocruz.br/handle/icict/1157910.1016/j.bjid.2012.10.019engBrazilian Society of Infectious DiseasesGenotyping and drug resistance patterns of Mycobacterium tuberculosis strains observed in a tuberculosis high-burden municipality in Northeast, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Ceará. Escola de Medicina. Departamento de Patologia, Fortaleza, CE, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias.. Rio de Janeiro, RJ, Brasil.Hospital de Messejana. Fortaleza, CE, Brasil.Universidade Federal do Ceará. Escola de Medicina. Departamento de Saúde Comunitária. Fortaleza, CE, Brasil.Universidade Federal do Ceará. Departamento de Geologia. Fortaleza, CE, Brasil.Universidade Federal do Ceará. Escola de Medicina. Departamento de Patologia, Fortaleza, CE, Brasil.Universidade Federal do Ceará. Departamento de Estatística e Matemática Aplicada. Fortaleza, CE, Brasil.Universidade Federal do Ceará. Escola de Medicina. Departamento de Patologia, Fortaleza, CE, Brasil.OBJECTIVES: This study has used a combination of clinical information, spoligotyping, and georeferencing system to elucidate the genetic diversity of the Mycobacterium tuberculosis isolates circulating in a TB-prevalent municipality of Northeast Brazil. METHODS: A total of 115 M. tuberculosis strains were isolated from pulmonary tuberculosis patients from January 2007 to March 2008 in Fortaleza. Drug susceptibility and spoligotyping assays were performed and place of residence of the patients were georeferenced. RESULTS: Of the M. tuberculosis strains studied, 51 (44.3%) isolates were resistant to at least one drug (R-TB) and 64 (55.7%) were sensitive to all the drugs tested (S-TB). A high frequency of resistance was found in previously treated cases (84%) and among new cases (16%; p < 0.001). a total of 74 (64%) isolates were grouped into 22 spoligotyped lineages, while 41 (36%) isolates were identified as new. among the predominant genotypes, 33% were latim american mediterranean (lam), 12% haarlem (h), and 5% u. there was no association of geographic distribution of rt-tb patients as compared to the controls and also the geographic location to the spoligotype patterns. the geospatial analysis revealed that 24 (23%) patients (hot spot zones) either shared the same residence or lived in a close neighborhood of a case. among these concentration zones, the patients lived in the same residence and shared a common genotype pattern and resistance pattern. DISCUSSION: it was observed that the spoligopatterns family distribution was similar to that reported for south america, prevailing the lam and h lineages. a high rate-case among the resistant TB group occurs as a result of transmitted and acquired resistance. A more effective surveillance program is needed in order to succeed in reducing tuberculosis in Northeast Brazil.TuberculosisDrug-resistant tuberculosisGeoreferencingSpoligotypingTuberculoseResistência a Medicamentosinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/11579/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALphilip_suffys_etal_IOC_2013.pdfapplication/pdf899409https://www.arca.fiocruz.br/bitstream/icict/11579/2/philip_suffys_etal_IOC_2013.pdf7ea9b7f69c1019e6b25fff232fcdaed7MD52TEXTphilip_suffys_etal_IOC_2013.pdf.txtphilip_suffys_etal_IOC_2013.pdf.txtExtracted texttext/plain34330https://www.arca.fiocruz.br/bitstream/icict/11579/3/philip_suffys_etal_IOC_2013.pdf.txtafc88eafe36af85066f6e3343bee4f1eMD53icict/115792022-06-24 12:19:37.75oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T15:19:37Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Genotyping and drug resistance patterns of Mycobacterium tuberculosis strains observed in a tuberculosis high-burden municipality in Northeast, Brazil |
| title |
Genotyping and drug resistance patterns of Mycobacterium tuberculosis strains observed in a tuberculosis high-burden municipality in Northeast, Brazil |
| spellingShingle |
Genotyping and drug resistance patterns of Mycobacterium tuberculosis strains observed in a tuberculosis high-burden municipality in Northeast, Brazil Luiz, Roberta dos Santos Silva Tuberculosis Drug-resistant tuberculosis Georeferencing Spoligotyping Tuberculose Resistência a Medicamentos |
| title_short |
Genotyping and drug resistance patterns of Mycobacterium tuberculosis strains observed in a tuberculosis high-burden municipality in Northeast, Brazil |
| title_full |
Genotyping and drug resistance patterns of Mycobacterium tuberculosis strains observed in a tuberculosis high-burden municipality in Northeast, Brazil |
| title_fullStr |
Genotyping and drug resistance patterns of Mycobacterium tuberculosis strains observed in a tuberculosis high-burden municipality in Northeast, Brazil |
| title_full_unstemmed |
Genotyping and drug resistance patterns of Mycobacterium tuberculosis strains observed in a tuberculosis high-burden municipality in Northeast, Brazil |
| title_sort |
Genotyping and drug resistance patterns of Mycobacterium tuberculosis strains observed in a tuberculosis high-burden municipality in Northeast, Brazil |
| author |
Luiz, Roberta dos Santos Silva |
| author_facet |
Luiz, Roberta dos Santos Silva Suffys, Phillip Barroso, Elizabeth Clara Kerr, Lígía Regina Franco Sansigolo Duarte, Cynthia Romariz Freitas, Max Victor Carioca Mota, Rosa Maria Salani Frota, Cristiane Cunha |
| author_role |
author |
| author2 |
Suffys, Phillip Barroso, Elizabeth Clara Kerr, Lígía Regina Franco Sansigolo Duarte, Cynthia Romariz Freitas, Max Victor Carioca Mota, Rosa Maria Salani Frota, Cristiane Cunha |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Luiz, Roberta dos Santos Silva Suffys, Phillip Barroso, Elizabeth Clara Kerr, Lígía Regina Franco Sansigolo Duarte, Cynthia Romariz Freitas, Max Victor Carioca Mota, Rosa Maria Salani Frota, Cristiane Cunha |
| dc.subject.en.pt_BR.fl_str_mv |
Tuberculosis Drug-resistant tuberculosis Georeferencing Spoligotyping |
| topic |
Tuberculosis Drug-resistant tuberculosis Georeferencing Spoligotyping Tuberculose Resistência a Medicamentos |
| dc.subject.decs.pt_BR.fl_str_mv |
Tuberculose Resistência a Medicamentos |
| description |
Universidade Federal do Ceará. Escola de Medicina. Departamento de Patologia, Fortaleza, CE, Brasil. |
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2013 |
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2013 |
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2015-08-19T13:49:35Z |
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2015-08-19T13:49:35Z |
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LUIZ, Roberta dos Santos Silva; et al. Genotyping and drug resistance patterns of Mycobacterium tuberculosis strains observed in a tuberculosis high-burden municipality in Northeast, Brazil. Braz J Infect Dis, v.17, n.3, p.338-345, June 2013. |
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1413-8670 |
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10.1016/j.bjid.2012.10.019 |
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LUIZ, Roberta dos Santos Silva; et al. Genotyping and drug resistance patterns of Mycobacterium tuberculosis strains observed in a tuberculosis high-burden municipality in Northeast, Brazil. Braz J Infect Dis, v.17, n.3, p.338-345, June 2013. 1413-8670 10.1016/j.bjid.2012.10.019 |
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Brazilian Society of Infectious Diseases |
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