Genotyping of Mycobacterium tuberculosis isolates from low endemic setting in south of Brazil

Detalhes bibliográficos
Autor(a) principal: Noguti, Erika Noda
Data de Publicação: 2009
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da Universidade Estadual de Maringá (RI-UEM)
Texto Completo: http://repositorio.uem.br:8080/jspui/handle/1/1901
Resumo: Tuberculosis (TB) is an important public health problem worldwide and the leading cause of deaths by an infectious agent. The objective of this study was provide information about genetic diversity of Mycobacterium tuberculosis, isolated from patients with pulmonary TB attended at the Laboratório de Ensino e Pesquisa em Análises Clínicas (LEPAC), using Spoligotyping and MIRU-VNTR methodologies. The results were reported in the paper "Genotyping of Mycobacterium tuberculosis isolates from low endemic setting in south of Brazil". From the Spoligotyping and MIRU-VNTR patterns of M. tuberculosis isolates dendrograms were constructed to evaluate the relationship of the isolates. The results show that TB in our region predominantly develops from endogenous reactivation of a latent infection. It was also carried out a comparison of discriminatory power between the methodologies used (Spoligotyping and MIRU-VNTR), obtaining better discriminatory capacity of the isolates combining the two methodologies.
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spelling Genotyping of Mycobacterium tuberculosis isolates from low endemic setting in south of BrazilTuberculoseMycobacterium tuberculosisEpidemiologia molecularSpoligotypingMIRUBrasil.TuberculosisMycobacterium tuberculosisMolecular EpidemiologySpoligotypingMIRUBrazil.Ciências da SaúdeFarmáciaTuberculosis (TB) is an important public health problem worldwide and the leading cause of deaths by an infectious agent. The objective of this study was provide information about genetic diversity of Mycobacterium tuberculosis, isolated from patients with pulmonary TB attended at the Laboratório de Ensino e Pesquisa em Análises Clínicas (LEPAC), using Spoligotyping and MIRU-VNTR methodologies. The results were reported in the paper "Genotyping of Mycobacterium tuberculosis isolates from low endemic setting in south of Brazil". From the Spoligotyping and MIRU-VNTR patterns of M. tuberculosis isolates dendrograms were constructed to evaluate the relationship of the isolates. The results show that TB in our region predominantly develops from endogenous reactivation of a latent infection. It was also carried out a comparison of discriminatory power between the methodologies used (Spoligotyping and MIRU-VNTR), obtaining better discriminatory capacity of the isolates combining the two methodologies.A tuberculose (TB) é um importante problema de saúde pública mundial, sendo a principal causa de mortes devido a um agente infeccioso. O objetivo desse trabalho foi fornecer informação sobre a diversidade genética de Mycobacterium tuberculosis, isolados de pacientes com TB pulmonar atendidos no Laboratório de Ensino e Pesquisa em Análises Clínicas (LEPAC), utilizando as metodologias Spoligotyping e MIRU-VNTR. Os resultados foram relatados no artigo "Genotyping of Mycobacterium tuberculosis isolates from low endemic setting in south of Brazil". Com os perfis de Spoligotyping e MIRU-VNTR dos isolados de M. tuberculosis foram construídos dendrogramas para avaliar o grau de relação entre os isolados. A partir desta avaliação pode-se verificar que a TB em nossa região desenvolve-se predominantemente devido à reativação endógena de uma infecção latente. Foi realizada também uma comparação da capacidade discriminatória entre as metodologias utilizadas (Spoligotyping e MIRU-VNTR), obtendo-se melhor capacidade discriminatória dos isolados combinando as duas metodologias.43 fUniversidade Estadual de MaringáBrasilDepartamento de Análises ClínicasPrograma de Pós-Graduação em Biociências Aplicadas à FarmáciaUEMMaringá, PRCentro de Ciências da SaúdeRosilene Fressatti CardosoThais Gomes Verzignassi Silveira - UEMEliana Valéria Patussi - UEMJosé Rodrigo Cláudio Pandolfi - Universidade Camilo Castelo BrancoDaisy Nakamura Sato - Instituto Adolfo LutzNoguti, Erika Noda2018-04-06T19:45:55Z2018-04-06T19:45:55Z2009info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesishttp://repositorio.uem.br:8080/jspui/handle/1/1901porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da Universidade Estadual de Maringá (RI-UEM)instname:Universidade Estadual de Maringá (UEM)instacron:UEM2018-10-08T17:20:27Zoai:localhost:1/1901Repositório InstitucionalPUBhttp://repositorio.uem.br:8080/oai/requestopendoar:2024-04-23T14:54:54.256309Repositório Institucional da Universidade Estadual de Maringá (RI-UEM) - Universidade Estadual de Maringá (UEM)false
dc.title.none.fl_str_mv Genotyping of Mycobacterium tuberculosis isolates from low endemic setting in south of Brazil
title Genotyping of Mycobacterium tuberculosis isolates from low endemic setting in south of Brazil
spellingShingle Genotyping of Mycobacterium tuberculosis isolates from low endemic setting in south of Brazil
Noguti, Erika Noda
Tuberculose
Mycobacterium tuberculosis
Epidemiologia molecular
Spoligotyping
MIRU
Brasil.
Tuberculosis
Mycobacterium tuberculosis
Molecular Epidemiology
Spoligotyping
MIRU
Brazil.
Ciências da Saúde
Farmácia
title_short Genotyping of Mycobacterium tuberculosis isolates from low endemic setting in south of Brazil
title_full Genotyping of Mycobacterium tuberculosis isolates from low endemic setting in south of Brazil
title_fullStr Genotyping of Mycobacterium tuberculosis isolates from low endemic setting in south of Brazil
title_full_unstemmed Genotyping of Mycobacterium tuberculosis isolates from low endemic setting in south of Brazil
title_sort Genotyping of Mycobacterium tuberculosis isolates from low endemic setting in south of Brazil
author Noguti, Erika Noda
author_facet Noguti, Erika Noda
author_role author
dc.contributor.none.fl_str_mv Rosilene Fressatti Cardoso
Thais Gomes Verzignassi Silveira - UEM
Eliana Valéria Patussi - UEM
José Rodrigo Cláudio Pandolfi - Universidade Camilo Castelo Branco
Daisy Nakamura Sato - Instituto Adolfo Lutz
dc.contributor.author.fl_str_mv Noguti, Erika Noda
dc.subject.por.fl_str_mv Tuberculose
Mycobacterium tuberculosis
Epidemiologia molecular
Spoligotyping
MIRU
Brasil.
Tuberculosis
Mycobacterium tuberculosis
Molecular Epidemiology
Spoligotyping
MIRU
Brazil.
Ciências da Saúde
Farmácia
topic Tuberculose
Mycobacterium tuberculosis
Epidemiologia molecular
Spoligotyping
MIRU
Brasil.
Tuberculosis
Mycobacterium tuberculosis
Molecular Epidemiology
Spoligotyping
MIRU
Brazil.
Ciências da Saúde
Farmácia
description Tuberculosis (TB) is an important public health problem worldwide and the leading cause of deaths by an infectious agent. The objective of this study was provide information about genetic diversity of Mycobacterium tuberculosis, isolated from patients with pulmonary TB attended at the Laboratório de Ensino e Pesquisa em Análises Clínicas (LEPAC), using Spoligotyping and MIRU-VNTR methodologies. The results were reported in the paper "Genotyping of Mycobacterium tuberculosis isolates from low endemic setting in south of Brazil". From the Spoligotyping and MIRU-VNTR patterns of M. tuberculosis isolates dendrograms were constructed to evaluate the relationship of the isolates. The results show that TB in our region predominantly develops from endogenous reactivation of a latent infection. It was also carried out a comparison of discriminatory power between the methodologies used (Spoligotyping and MIRU-VNTR), obtaining better discriminatory capacity of the isolates combining the two methodologies.
publishDate 2009
dc.date.none.fl_str_mv 2009
2018-04-06T19:45:55Z
2018-04-06T19:45:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.uem.br:8080/jspui/handle/1/1901
url http://repositorio.uem.br:8080/jspui/handle/1/1901
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade Estadual de Maringá
Brasil
Departamento de Análises Clínicas
Programa de Pós-Graduação em Biociências Aplicadas à Farmácia
UEM
Maringá, PR
Centro de Ciências da Saúde
publisher.none.fl_str_mv Universidade Estadual de Maringá
Brasil
Departamento de Análises Clínicas
Programa de Pós-Graduação em Biociências Aplicadas à Farmácia
UEM
Maringá, PR
Centro de Ciências da Saúde
dc.source.none.fl_str_mv reponame:Repositório Institucional da Universidade Estadual de Maringá (RI-UEM)
instname:Universidade Estadual de Maringá (UEM)
instacron:UEM
instname_str Universidade Estadual de Maringá (UEM)
instacron_str UEM
institution UEM
reponame_str Repositório Institucional da Universidade Estadual de Maringá (RI-UEM)
collection Repositório Institucional da Universidade Estadual de Maringá (RI-UEM)
repository.name.fl_str_mv Repositório Institucional da Universidade Estadual de Maringá (RI-UEM) - Universidade Estadual de Maringá (UEM)
repository.mail.fl_str_mv
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