Comparison of Dengue Infection in Human Mononuclear Leukocytes with Mosquito C6/36 and Mammalian Vero Cells Using Flow Cytometry to Detect Virus Antigen
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2000 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/44084 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil. |
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von Sydow, Farid F. O .Santiago, Marta A.Souza, Patricia C. NevesCerqueira, Denise I. S .Gouvea, Adriana S.Lavatori, Maryrose F. H.Bertho, Álvaro L.Kubelka, Claire F.2020-10-20T17:39:51Z2020-10-20T17:39:51Z2000von Sydow, Farid F. O. et al. Comparison of Dengue Infection in Human Mononuclear Leukocytes with Mosquito C6/36 and Mammalian Vero Cells Using Flow Cytometry to Detect Virus Antigen. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 95, n. 4, p. 483-489, 2000.0074-0276https://www.arca.fiocruz.br/handle/icict/4408410.1590/S0074-027620000004000071678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.Vírus da DengueCitometria de fluxoCélulas veroLeucócitos mononuclearesC6/36Dengue virusC6/36Vero cellsMononuclear leukocytesFlow cytometryComparison of Dengue Infection in Human Mononuclear Leukocytes with Mosquito C6/36 and Mammalian Vero Cells Using Flow Cytometry to Detect Virus Antigeninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.Fluorescent activated cell sorter (FACS) analysis is useful for the detection of cellular surface antigens and intracellular proteins. We used this methodology in order to detect and quantify dengue antigens in highly susceptible cells such as clone C6/36 (Aedes albopictus) and Vero cells (green monkey kidney). Additionally, we analyzed the infection in vitro of human peripheral blood mononuclear leukocytes (PBML). FACS analysis turned out to be a reliable technique to quantify virus growth in traditional cell cultures of C6/36 as well as Vero cells. High rates of infection were achieved with a good statistical correlation between the virus amount used in infection and the percentage of dengue antigen containing cells detected in infected cultures. We also showed that human monocytes (CD14+) are preferred target cells for in vitro dengue infection among PBML. Monocytes were much less susceptible to virus infection than cell lines but they displayed dengue antigens detected by FACS five days after infection. In contrast, lymphocytes showed no differences in their profile for dengue specific immunofluorescence. Without an animal model to reproduce dengue disease, alternative assays have been sought to correlate viral virulence with clinical manifestations and disease severity. Study of in vitro interaction of virus and host cells may highlight this relationship.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/44084/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALClaireF_Kubelka_etal_IOC_2000.pdfClaireF_Kubelka_etal_IOC_2000.pdfapplication/pdf84454https://www.arca.fiocruz.br/bitstream/icict/44084/2/ClaireF_Kubelka_etal_IOC_2000.pdf5598def8eae8d8b98c8d39aa03020fbaMD52TEXTClaireF_Kubelka_etal_IOC_2000.pdf.txtClaireF_Kubelka_etal_IOC_2000.pdf.txtExtracted texttext/plain28041https://www.arca.fiocruz.br/bitstream/icict/44084/3/ClaireF_Kubelka_etal_IOC_2000.pdf.txt96be283aaebbd7ea6978d8e5823f791fMD53icict/440842020-10-21 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| dc.title.pt_BR.fl_str_mv |
Comparison of Dengue Infection in Human Mononuclear Leukocytes with Mosquito C6/36 and Mammalian Vero Cells Using Flow Cytometry to Detect Virus Antigen |
| title |
Comparison of Dengue Infection in Human Mononuclear Leukocytes with Mosquito C6/36 and Mammalian Vero Cells Using Flow Cytometry to Detect Virus Antigen |
| spellingShingle |
Comparison of Dengue Infection in Human Mononuclear Leukocytes with Mosquito C6/36 and Mammalian Vero Cells Using Flow Cytometry to Detect Virus Antigen von Sydow, Farid F. O . Vírus da Dengue Citometria de fluxo Células vero Leucócitos mononucleares C6/36 Dengue virus C6/36 Vero cells Mononuclear leukocytes Flow cytometry |
| title_short |
Comparison of Dengue Infection in Human Mononuclear Leukocytes with Mosquito C6/36 and Mammalian Vero Cells Using Flow Cytometry to Detect Virus Antigen |
| title_full |
Comparison of Dengue Infection in Human Mononuclear Leukocytes with Mosquito C6/36 and Mammalian Vero Cells Using Flow Cytometry to Detect Virus Antigen |
| title_fullStr |
Comparison of Dengue Infection in Human Mononuclear Leukocytes with Mosquito C6/36 and Mammalian Vero Cells Using Flow Cytometry to Detect Virus Antigen |
| title_full_unstemmed |
Comparison of Dengue Infection in Human Mononuclear Leukocytes with Mosquito C6/36 and Mammalian Vero Cells Using Flow Cytometry to Detect Virus Antigen |
| title_sort |
Comparison of Dengue Infection in Human Mononuclear Leukocytes with Mosquito C6/36 and Mammalian Vero Cells Using Flow Cytometry to Detect Virus Antigen |
| author |
von Sydow, Farid F. O . |
| author_facet |
von Sydow, Farid F. O . Santiago, Marta A. Souza, Patricia C. Neves Cerqueira, Denise I. S . Gouvea, Adriana S. Lavatori, Maryrose F. H. Bertho, Álvaro L. Kubelka, Claire F. |
| author_role |
author |
| author2 |
Santiago, Marta A. Souza, Patricia C. Neves Cerqueira, Denise I. S . Gouvea, Adriana S. Lavatori, Maryrose F. H. Bertho, Álvaro L. Kubelka, Claire F. |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
von Sydow, Farid F. O . Santiago, Marta A. Souza, Patricia C. Neves Cerqueira, Denise I. S . Gouvea, Adriana S. Lavatori, Maryrose F. H. Bertho, Álvaro L. Kubelka, Claire F. |
| dc.subject.other.pt_BR.fl_str_mv |
Vírus da Dengue Citometria de fluxo Células vero Leucócitos mononucleares C6/36 |
| topic |
Vírus da Dengue Citometria de fluxo Células vero Leucócitos mononucleares C6/36 Dengue virus C6/36 Vero cells Mononuclear leukocytes Flow cytometry |
| dc.subject.en.pt_BR.fl_str_mv |
Dengue virus C6/36 Vero cells Mononuclear leukocytes Flow cytometry |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil. |
| publishDate |
2000 |
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2000 |
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2020-10-20T17:39:51Z |
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2020-10-20T17:39:51Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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von Sydow, Farid F. O. et al. Comparison of Dengue Infection in Human Mononuclear Leukocytes with Mosquito C6/36 and Mammalian Vero Cells Using Flow Cytometry to Detect Virus Antigen. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 95, n. 4, p. 483-489, 2000. |
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https://www.arca.fiocruz.br/handle/icict/44084 |
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0074-0276 |
| dc.identifier.doi.none.fl_str_mv |
10.1590/S0074-02762000000400007 |
| dc.identifier.eissn.none.fl_str_mv |
1678-8060 |
| identifier_str_mv |
von Sydow, Farid F. O. et al. Comparison of Dengue Infection in Human Mononuclear Leukocytes with Mosquito C6/36 and Mammalian Vero Cells Using Flow Cytometry to Detect Virus Antigen. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 95, n. 4, p. 483-489, 2000. 0074-0276 10.1590/S0074-02762000000400007 1678-8060 |
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Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. |
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Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. |
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