Vesicles from different Trypanosoma cruzi strains trigger differential innate and chronic immune responses
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2015 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/15736 |
Resumo: | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte,MG, Brasil/Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil |
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Nogueira, Paula MonalisaRibeiro, KleberSilveira, Amanda Cardoso de OliveiraMartins Filho, Olindo AssisBela, Samantha RibeiroCampos, Marco Antônio SilvaPessoa, Natalia LimaColli, WalterAlves, Maria Julia MansoSoares, Rodrigo Pedro PintoTorrecilhas, Ana Claudia2016-09-08T18:36:59Z2016-09-08T18:36:59Z2015NOGUEIRA, Paula Monalisa et al. Vesicles from different Trypanosoma cruzi strains trigger differential innate and chronic immune responses. J Extracell Vesicles. Vol. 4, p. 28734, 2015.2001-3078https://www.arca.fiocruz.br/handle/icict/1573610.3402/jev.v4.28734. eCollection 2015engCo-Action PubVesicles from different Trypanosoma cruzi strains trigger differential innate and chronic immune responsesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte,MG, Brasil/Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrasilUniversidade Federal de São Paulo. Departamento de Ciências Biológicas. Laboratório de Imunologia Celular e Bioquímica de Fungos e Protozoários. São Paulo, SP, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrasilUniversidade Federal de São Paulo. Departamento de Ciências Biológicas. Laboratório de Imunologia Celular e Bioquímica de Fungos e Protozoários. São Paulo, SP, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrasilUniversidade de São Paulo. Instituto de Química. Departamento de Bioquímica. São Paulo, SP, BrasilUniversidade de São Paulo. Instituto de Química. Departamento de Bioquímica. São Paulo, SP, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte,MG, Brasil/Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrasilUniversidade Federal de São Paulo. Departamento de Ciências Biológicas. Laboratório de Imunologia Celular e Bioquímica de Fungos e Protozoários. São Paulo, SP, BrasilTrypomastigote forms of Trypanosoma cruzi, the causative agent of Chagas Disease, shed extracellular vesicles (EVs) enriched with glycoproteins of the gp85/trans-sialidase (TS) superfamily and other α-galactosyl (α-Gal)-containing glycoconjugates, such as mucins. Here, purified vesicles from T. cruzi strains (Y, Colombiana, CL-14 and YuYu) were quantified according to size, intensity and concentration. Qualitative analysis revealed differences in their protein and α-galactosyl contents. Later, those polymorphisms were evaluated in the modulation of immune responses (innate and in the chronic phase) in C57BL/6 mice. EVs isolated from YuYu and CL-14 strains induced in macrophages higher levels of proinflammatory cytokines (TNF-α and IL-6) and nitric oxide via TLR2. In general, no differences were observed in MAPKs activation (p38, JNK and ERK 1/2) after EVs stimulation. In splenic cells derived from chronically infected mice, a different modulation pattern was observed, where Colombiana (followed by Y strain) EVs were more proinflammatory. This modulation was independent of the T. cruzi strain used in the mice infection. To test the functional importance of this modulation, the expression of intracellular cytokines after in vitro exposure was evaluated using EVs from YuYu and Colombiana strains. Both EVs induced cytokine production with the appearance of IL-10 in the chronically infected mice. A high frequency of IL-10 in CD4+ and CD8+ T lymphocytes was observed. A mixed profile of cytokine induction was observed in B cells with the production of TNF-α and IL-10. Finally, dendritic cells produced TNF-α after stimulation with EVs. Polymorphisms in the vesicles surface may be determinant in the immunopathologic events not only in the early steps of infection but also in the chronic phase.TLR2Trypanosoma cruziextracellular vesiclesinnate and chronic immunityα-galactosylinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/15736/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALve_Paula_Nogueira_Vesicles_CPqRR_2015.pdfve_Paula_Nogueira_Vesicles_CPqRR_2015.pdfapplication/pdf7053221https://www.arca.fiocruz.br/bitstream/icict/15736/2/ve_Paula_Nogueira_Vesicles_CPqRR_2015.pdf38b07d08c52b86bcd4b207e402020ad9MD52TEXTve_Paula_Nogueira_Vesicles_CPqRR_2015.pdf.txtve_Paula_Nogueira_Vesicles_CPqRR_2015.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Vesicles from different Trypanosoma cruzi strains trigger differential innate and chronic immune responses |
| title |
Vesicles from different Trypanosoma cruzi strains trigger differential innate and chronic immune responses |
| spellingShingle |
Vesicles from different Trypanosoma cruzi strains trigger differential innate and chronic immune responses Nogueira, Paula Monalisa TLR2 Trypanosoma cruzi extracellular vesicles innate and chronic immunity α-galactosyl |
| title_short |
Vesicles from different Trypanosoma cruzi strains trigger differential innate and chronic immune responses |
| title_full |
Vesicles from different Trypanosoma cruzi strains trigger differential innate and chronic immune responses |
| title_fullStr |
Vesicles from different Trypanosoma cruzi strains trigger differential innate and chronic immune responses |
| title_full_unstemmed |
Vesicles from different Trypanosoma cruzi strains trigger differential innate and chronic immune responses |
| title_sort |
Vesicles from different Trypanosoma cruzi strains trigger differential innate and chronic immune responses |
| author |
Nogueira, Paula Monalisa |
| author_facet |
Nogueira, Paula Monalisa Ribeiro, Kleber Silveira, Amanda Cardoso de Oliveira Martins Filho, Olindo Assis Bela, Samantha Ribeiro Campos, Marco Antônio Silva Pessoa, Natalia Lima Colli, Walter Alves, Maria Julia Manso Soares, Rodrigo Pedro Pinto Torrecilhas, Ana Claudia |
| author_role |
author |
| author2 |
Ribeiro, Kleber Silveira, Amanda Cardoso de Oliveira Martins Filho, Olindo Assis Bela, Samantha Ribeiro Campos, Marco Antônio Silva Pessoa, Natalia Lima Colli, Walter Alves, Maria Julia Manso Soares, Rodrigo Pedro Pinto Torrecilhas, Ana Claudia |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Nogueira, Paula Monalisa Ribeiro, Kleber Silveira, Amanda Cardoso de Oliveira Martins Filho, Olindo Assis Bela, Samantha Ribeiro Campos, Marco Antônio Silva Pessoa, Natalia Lima Colli, Walter Alves, Maria Julia Manso Soares, Rodrigo Pedro Pinto Torrecilhas, Ana Claudia |
| dc.subject.en.pt_BR.fl_str_mv |
TLR2 Trypanosoma cruzi extracellular vesicles innate and chronic immunity α-galactosyl |
| topic |
TLR2 Trypanosoma cruzi extracellular vesicles innate and chronic immunity α-galactosyl |
| description |
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte,MG, Brasil/Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil |
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2015 |
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2015 |
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2016-09-08T18:36:59Z |
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2016-09-08T18:36:59Z |
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NOGUEIRA, Paula Monalisa et al. Vesicles from different Trypanosoma cruzi strains trigger differential innate and chronic immune responses. J Extracell Vesicles. Vol. 4, p. 28734, 2015. |
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https://www.arca.fiocruz.br/handle/icict/15736 |
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2001-3078 |
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10.3402/jev.v4.28734. eCollection 2015 |
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NOGUEIRA, Paula Monalisa et al. Vesicles from different Trypanosoma cruzi strains trigger differential innate and chronic immune responses. J Extracell Vesicles. Vol. 4, p. 28734, 2015. 2001-3078 10.3402/jev.v4.28734. eCollection 2015 |
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