Landscape of the spliced leader trans-splicing mechanism in Schistosoma mansoni
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/33670 |
Resumo: | Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Laboratório de Genética Bioquímica. Belo Horizonte, MG, Brasil / Instituto Nacional de Câncer José Alencar Gomes da Silva. Coordenação de Pesquisa. Laboratório de Bioinformática e Biologia Computacional. Rio de Janeiro, RJ, Brasil. |
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Boroni, MarianaSammeth, MichaelGava, Sandra GrossiJorge, Natasha Andressa NogueiraMacedo, Andréa MaraMachado, Carlos RenatoMourão, Marina MoraesFranco, Glória Regina2019-06-25T19:33:05Z2019-06-25T19:33:05Z2018BORONI, Mariana et al. Landscape of the spliced leader trans-splicing mechanism in Schistosoma mansoni. Scientific Reports, v. 8, n. 1, p. 1-14, 2018.2045-2322https://www.arca.fiocruz.br/handle/icict/3367010.1038/s41598-018-22093-3engNature Publishing GroupEsquistossomosseSchistosoma mansoniLandscape of the spliced leader trans-splicing mechanism in Schistosoma mansoniinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Laboratório de Genética Bioquímica. Belo Horizonte, MG, Brasil / Instituto Nacional de Câncer José Alencar Gomes da Silva. Coordenação de Pesquisa. Laboratório de Bioinformática e Biologia Computacional. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Bioinformática em Transcriptômica e Genômica Funcional.Rio de Janeiro, MG, Brasil / Laboratório Nacional de Computação Científica. Petrópolis, RJ, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Helmintologia e Malacologia Médica. Belo Horizonte, MG, Brasil.Instituto Nacional de Câncer José Alencar Gomes da Silva. Coordenação de Pesquisa. Laboratório de Bioinformática e Biologia Computacional. Rio de Janeiro, RJ, Brasil.Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Laboratório de Genética Bioquímica. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Laboratório de Genética Bioquímica. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Helmintologia e Malacologia Médica. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Laboratório de Genética Bioquímica. Belo Horizonte, MG, Brasil.Spliced leader dependent trans-splicing (SLTS) has been described as an important RNA regulatory process that occurs in different organisms, including the trematode Schistosoma mansoni. We identified more than seven thousand putative SLTS sites in the parasite, comprising genes with a wide spectrum of functional classes, which underlines the SLTS as a ubiquitous mechanism in the parasite. Also, SLTS gene expression levels span several orders of magnitude, showing that SLTS frequency is not determined by the expression level of the target gene, but by the presence of particular gene features facilitating or hindering the trans-splicing mechanism. Our in-depth investigation of SLTS events demonstrates widespread alternative trans-splicing (ATS) acceptor sites occurring in different regions along the entire gene body, highlighting another important role of SLTS generating alternative RNA isoforms in the parasite, besides the polycistron resolution. Particularly for introns where SLTS directly competes for the same acceptor substrate with cis-splicing, we identified for the first time additional and important features that might determine the type of splicing. Our study substantially extends the current knowledge of RNA processing by SLTS in S. mansoni, and provide basis for future studies on the trans-splicing mechanism in other eukaryotes.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/33670/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALLandscape of the spliced leader .pdfLandscape of the spliced leader .pdfapplication/pdf9242297https://www.arca.fiocruz.br/bitstream/icict/33670/2/Landscape%20of%20the%20spliced%20leader%20.pdf760922aef628f39e3bb669dc8249cd5bMD52TEXTLandscape of the spliced leader .pdf.txtLandscape of the spliced leader .pdf.txtExtracted texttext/plain58https://www.arca.fiocruz.br/bitstream/icict/33670/3/Landscape%20of%20the%20spliced%20leader%20.pdf.txt764cf8f0a04ca429706164eb20f9b584MD53icict/336702019-06-30 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| dc.title.pt_BR.fl_str_mv |
Landscape of the spliced leader trans-splicing mechanism in Schistosoma mansoni |
| title |
Landscape of the spliced leader trans-splicing mechanism in Schistosoma mansoni |
| spellingShingle |
Landscape of the spliced leader trans-splicing mechanism in Schistosoma mansoni Boroni, Mariana Esquistossomosse Schistosoma mansoni |
| title_short |
Landscape of the spliced leader trans-splicing mechanism in Schistosoma mansoni |
| title_full |
Landscape of the spliced leader trans-splicing mechanism in Schistosoma mansoni |
| title_fullStr |
Landscape of the spliced leader trans-splicing mechanism in Schistosoma mansoni |
| title_full_unstemmed |
Landscape of the spliced leader trans-splicing mechanism in Schistosoma mansoni |
| title_sort |
Landscape of the spliced leader trans-splicing mechanism in Schistosoma mansoni |
| author |
Boroni, Mariana |
| author_facet |
Boroni, Mariana Sammeth, Michael Gava, Sandra Grossi Jorge, Natasha Andressa Nogueira Macedo, Andréa Mara Machado, Carlos Renato Mourão, Marina Moraes Franco, Glória Regina |
| author_role |
author |
| author2 |
Sammeth, Michael Gava, Sandra Grossi Jorge, Natasha Andressa Nogueira Macedo, Andréa Mara Machado, Carlos Renato Mourão, Marina Moraes Franco, Glória Regina |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Boroni, Mariana Sammeth, Michael Gava, Sandra Grossi Jorge, Natasha Andressa Nogueira Macedo, Andréa Mara Machado, Carlos Renato Mourão, Marina Moraes Franco, Glória Regina |
| dc.subject.other.pt_BR.fl_str_mv |
Esquistossomosse |
| topic |
Esquistossomosse Schistosoma mansoni |
| dc.subject.en.pt_BR.fl_str_mv |
Schistosoma mansoni |
| description |
Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Laboratório de Genética Bioquímica. Belo Horizonte, MG, Brasil / Instituto Nacional de Câncer José Alencar Gomes da Silva. Coordenação de Pesquisa. Laboratório de Bioinformática e Biologia Computacional. Rio de Janeiro, RJ, Brasil. |
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2018 |
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2018 |
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2019-06-25T19:33:05Z |
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2019-06-25T19:33:05Z |
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BORONI, Mariana et al. Landscape of the spliced leader trans-splicing mechanism in Schistosoma mansoni. Scientific Reports, v. 8, n. 1, p. 1-14, 2018. |
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https://www.arca.fiocruz.br/handle/icict/33670 |
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2045-2322 |
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10.1038/s41598-018-22093-3 |
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BORONI, Mariana et al. Landscape of the spliced leader trans-splicing mechanism in Schistosoma mansoni. Scientific Reports, v. 8, n. 1, p. 1-14, 2018. 2045-2322 10.1038/s41598-018-22093-3 |
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Nature Publishing Group |
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