Genome-wide identification, characterisation and expression profiling of the ubiquitin-proteasome genes in Biomphalaria glabrata

Detalhes bibliográficos
Autor(a) principal: Portilho, Laysa Gomes
Data de Publicação: 2019
Outros Autores: Duarte, Bruna Custódio Dias, Queiroz, Fábio Ribeiro, Ribeiro, Thales Henrique Cherubino, Jeremias, Wander de Jesus, Babá, Elio Hideo, Coelho, Paulo Marcos Zech, Morais, Enyara Rezende, Cabral, Fernanda Janku, Caldeira, Roberta Lima, Gomes, Matheus de Souza
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/35124
Resumo: Universidade Federal de Uberlândia. Laboratório de Bioinformática e Análises Moleculares. Patos de Minas, MG, Brasil.
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spelling Portilho, Laysa GomesDuarte, Bruna Custódio DiasQueiroz, Fábio RibeiroRibeiro, Thales Henrique CherubinoJeremias, Wander de JesusBabá, Elio HideoCoelho, Paulo Marcos ZechMorais, Enyara RezendeCabral, Fernanda JankuCaldeira, Roberta LimaGomes, Matheus de Souza2019-08-27T16:43:34Z2019-08-27T16:43:34Z2019PORTILHO, Laysa Gomes et al. Genome-wide identification, characterisation and expression profiling of the ubiquitin-proteasome genes in Biomphalaria glabrata. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 114, p. 1-15, 2019.0074-0276https://www.arca.fiocruz.br/handle/icict/3512410.1590/0074-02760190052engFundação Oswaldo CruzGenome-wide identification, characterisation and expression profiling of the ubiquitin-proteasome genes in Biomphalaria glabratainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Uberlândia. Laboratório de Bioinformática e Análises Moleculares. Patos de Minas, MG, Brasil.Universidade Federal de Uberlândia. Laboratório de Bioinformática e Análises Moleculares. Patos de Minas, MG, BrasilFundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Biologia do Schistosoma mansoni e sua Interação com o Hospedeiro. Belo Horizonte, MG, Brasil.Universidade Federal de Lavras. Departamento de Biologia. Seção de Fisiologia de Plantas. Laboratório de Fisiologia Molecular de Plantas. Lavras, MG, Brasil.Universidade Federal de Ouro Preto. Escola de Farmácia. Departamento de Farmácia. Ouro Preto, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Biologia do Schistosoma mansoni e sua Interação com o Hospedeiro. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Biologia do Schistosoma mansoni e sua Interação com o Hospedeiro. Belo Horizonte, MG, Brasil.Universidade Federal de Uberlândia. Laboratório de Bioquímica e Biologia Molecular. Patos de Minas, MG, Brasil.Universidade Estadual de Campinas. Instituto de Biologia. Departamento de Biologia Animal. Campinas, SP, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Helmintologia e Malacologia Médica. Belo Horizonte, MG, Brasil.Universidade Federal de Uberlândia. Laboratório de Bioinformática e Análises Moleculares. Patos de Minas, MG, Brasil.BACKGROUND: Biomphalaria glabrata is the major species used for the study of schistosomiasis-related parasite-host relationships, and understanding its gene regulation may aid in this endeavor. The ubiquitin-proteasome system (UPS) performs post-translational regulation in order to maintain cellular protein homeostasis and is related to several mechanisms, including immune responses. OBJECTIVE: The aims of this work were to identify and characterise the putative genes and proteins involved in UPS using bioinformatic tools and also their expression on different tissues of B. glabrata. METHODS: The putative genes and proteins of UPS in B. glabrata were predicted using BLASTp and as queries reference proteins from model organism. We characterised these putative proteins using PFAM and CDD software describing the conserved domains and active sites. The phylogenetic analysis was performed using ClustalX2 and MEGA5.2. Expression evaluation was performed from 12 snail tissues using RPKM. FINDINGS: 119 sequences involved in the UPS in B. glabrata were identified, which 86 have been related to the ubiquitination pathway and 33 to proteasome. In addition, the conserved domains found were associated with the ubiquitin family, UQ_con, HECT, U-box and proteasome. The main active sites were lysine and cysteine residues. Lysines are responsible and the starting point for the formation of polyubiquitin chains, while the cysteine residues of the enzymes are responsible for binding to ubiquitin. The phylogenetic analysis showed an organised distribution between the organisms and the clades of the sequences, corresponding to the tree of life of the animals, for all groups of sequences analysed. The ubiquitin sequence was the only one with a high expression profile found in all libraries, inferring its wide range of performance. MAIN CONCLUSIONS: Our results show the presence, conservation and expression profile of the UPS in this mollusk, providing a basis and new knowledge for other studies involving this system. Due to the importance of the UPS and B. glabrata, this work may influence the search for new methodologies for the control of schistosomiasis.SnailUPSSignaling pathwayBioinformaticsSchistosomiasisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/35124/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALGenome-wide identification.pdfGenome-wide identification.pdfapplication/pdf12612955https://www.arca.fiocruz.br/bitstream/icict/35124/2/Genome-wide%20identification.pdffd4068e8355f870120d20ad7ff2c2fc6MD52TEXTGenome-wide identification.pdf.txtGenome-wide identification.pdf.txtExtracted texttext/plain218https://www.arca.fiocruz.br/bitstream/icict/35124/3/Genome-wide%20identification.pdf.txtc0fec3e4d468323350ba24b8f5272cc6MD53icict/351242019-08-30 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dc.title.pt_BR.fl_str_mv Genome-wide identification, characterisation and expression profiling of the ubiquitin-proteasome genes in Biomphalaria glabrata
title Genome-wide identification, characterisation and expression profiling of the ubiquitin-proteasome genes in Biomphalaria glabrata
spellingShingle Genome-wide identification, characterisation and expression profiling of the ubiquitin-proteasome genes in Biomphalaria glabrata
Portilho, Laysa Gomes
Snail
UPS
Signaling pathway
Bioinformatics
Schistosomiasis
title_short Genome-wide identification, characterisation and expression profiling of the ubiquitin-proteasome genes in Biomphalaria glabrata
title_full Genome-wide identification, characterisation and expression profiling of the ubiquitin-proteasome genes in Biomphalaria glabrata
title_fullStr Genome-wide identification, characterisation and expression profiling of the ubiquitin-proteasome genes in Biomphalaria glabrata
title_full_unstemmed Genome-wide identification, characterisation and expression profiling of the ubiquitin-proteasome genes in Biomphalaria glabrata
title_sort Genome-wide identification, characterisation and expression profiling of the ubiquitin-proteasome genes in Biomphalaria glabrata
author Portilho, Laysa Gomes
author_facet Portilho, Laysa Gomes
Duarte, Bruna Custódio Dias
Queiroz, Fábio Ribeiro
Ribeiro, Thales Henrique Cherubino
Jeremias, Wander de Jesus
Babá, Elio Hideo
Coelho, Paulo Marcos Zech
Morais, Enyara Rezende
Cabral, Fernanda Janku
Caldeira, Roberta Lima
Gomes, Matheus de Souza
author_role author
author2 Duarte, Bruna Custódio Dias
Queiroz, Fábio Ribeiro
Ribeiro, Thales Henrique Cherubino
Jeremias, Wander de Jesus
Babá, Elio Hideo
Coelho, Paulo Marcos Zech
Morais, Enyara Rezende
Cabral, Fernanda Janku
Caldeira, Roberta Lima
Gomes, Matheus de Souza
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Portilho, Laysa Gomes
Duarte, Bruna Custódio Dias
Queiroz, Fábio Ribeiro
Ribeiro, Thales Henrique Cherubino
Jeremias, Wander de Jesus
Babá, Elio Hideo
Coelho, Paulo Marcos Zech
Morais, Enyara Rezende
Cabral, Fernanda Janku
Caldeira, Roberta Lima
Gomes, Matheus de Souza
dc.subject.en.pt_BR.fl_str_mv Snail
UPS
Signaling pathway
Bioinformatics
Schistosomiasis
topic Snail
UPS
Signaling pathway
Bioinformatics
Schistosomiasis
description Universidade Federal de Uberlândia. Laboratório de Bioinformática e Análises Moleculares. Patos de Minas, MG, Brasil.
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-08-27T16:43:34Z
dc.date.available.fl_str_mv 2019-08-27T16:43:34Z
dc.date.issued.fl_str_mv 2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PORTILHO, Laysa Gomes et al. Genome-wide identification, characterisation and expression profiling of the ubiquitin-proteasome genes in Biomphalaria glabrata. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 114, p. 1-15, 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/35124
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/0074-02760190052
identifier_str_mv PORTILHO, Laysa Gomes et al. Genome-wide identification, characterisation and expression profiling of the ubiquitin-proteasome genes in Biomphalaria glabrata. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 114, p. 1-15, 2019.
0074-0276
10.1590/0074-02760190052
url https://www.arca.fiocruz.br/handle/icict/35124
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/35124/1/license.txt
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