Genome-wide identification, characterization and expression profiling of the ubiquitin-proteasome genes in Biomphalaria glabrata

Detalhes bibliográficos
Autor(a) principal: Duarte, Bruna Custódio Dias
Data de Publicação: 2019
Tipo de documento: Trabalho de conclusão de curso
Idioma: por
Título da fonte: Repositório Institucional da UFU
Texto Completo: https://repositorio.ufu.br/handle/123456789/27815
Resumo: Trabalho de Conclusão de Curso (Graduação)
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spelling Genome-wide identification, characterization and expression profiling of the ubiquitin-proteasome genes in Biomphalaria glabrataIdentificação em todo o genoma, caracterização e perfil de expressão dos genes de ubiquitina-proteassoma em Biomphalaria glabrataSnailCaramujoUPSSignaling pathwayVia de sinalizaçãoBioinformaticsBioinformáticaSchistosomiasisEsquistossomoseCNPQ::CIENCIAS BIOLOGICAS::PARASITOLOGIATrabalho de Conclusão de Curso (Graduação)Abstract BACKGROUND: Biomphalaria glabrata is the major species used for the study of schistosomiasis-related parasite-host relationships, and understanding its gene regulation may aid in this endeavor. The ubiquitin-proteasome system (UPS) performs post-translational regulation in order to maintain cellular protein homeostasis and is related to several mechanisms, including immune responses. OBJECTIVE: The aims of this work were to identify and characterize the putative genes and proteins involved in UPS using bioinformatic tools and also their expression on different tissues of B. glabrata. METHODS: The putative genes and proteins of UPS in B. glabrata were predicted using BLASTp and as queries reference proteins from model organism. We characterized these putative proteins using PFAM and CDD software describing the conserved domains and active sites. The phylogenetic analysis was performed using ClustalX2 and MEGA5.2. Expression evaluation was performed from 12 snail tissue using RPKM. FINDINGS: 119 sequences involved in the UPS in B. glabrata were identified, which 86 have been related to the ubiquitination pathway and 33 to proteasome. In addition, the conserved domains found were associated with the ubiquitin family, UQ_con, HECT, U-box and proteasome. The main active sites were lysine and cysteine residues. Lysines are responsible and the starting point for the formation of polyubiquitin chains, while the cysteine residues of the enzymes are responsible for binding to ubiquitin. The phylogenetic analysis showed an organised distribution between the organisms and the clades of the sequences, corresponding to the tree of life of the animals, for all groups of sequences analysed. The ubiquitin sequence was the only one with a high expression profile found in all libraries, inferring its wide range of performance. MAIN CONCLUSIONS: Our results evidenced the presence, conservation and expression profile of the UPS in this mollusk, providing basis and new knowledge to other studies involving this system. Due to the importance of UPS and B. glabrata, this work may influence the search for new methodologies for the control of schistosomiasis.Universidade Federal de UberlândiaBrasilBiotecnologiaGomes, Matheus de Souzahttp://lattes.cnpq.br/8674610062329213Barbosa, Aulus Estevão Anjos de Deushttp://lattes.cnpq.br/5331406701784638Freitas, Guilherme Ramos Oliveira ehttp://lattes.cnpq.br/4014066906990228Duarte, Bruna Custódio Dias2019-12-18T19:12:34Z2019-12-18T19:12:34Z2019-12-11info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesisapplication/pdfapplication/pdfapplication/pdfDUARTE, Bruna Custódio Dias. Genome-wide identification, characterization and expression profiling of the ubiquitin-proteasome genes in Biomphalaria glabrata. 2019. 46 f. Trabalho de Conclusão de Curso (Graduação em Biotecnologia) - Universidade Federal de Uberlândia, Patos de Minas, 2019.https://repositorio.ufu.br/handle/123456789/27815porhttp://creativecommons.org/licenses/by-nc-nd/3.0/us/info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFUinstname:Universidade Federal de Uberlândia (UFU)instacron:UFU2019-12-19T06:13:38Zoai:repositorio.ufu.br:123456789/27815Repositório InstitucionalONGhttp://repositorio.ufu.br/oai/requestdiinf@dirbi.ufu.bropendoar:2019-12-19T06:13:38Repositório Institucional da UFU - Universidade Federal de Uberlândia (UFU)false
dc.title.none.fl_str_mv Genome-wide identification, characterization and expression profiling of the ubiquitin-proteasome genes in Biomphalaria glabrata
Identificação em todo o genoma, caracterização e perfil de expressão dos genes de ubiquitina-proteassoma em Biomphalaria glabrata
title Genome-wide identification, characterization and expression profiling of the ubiquitin-proteasome genes in Biomphalaria glabrata
spellingShingle Genome-wide identification, characterization and expression profiling of the ubiquitin-proteasome genes in Biomphalaria glabrata
Duarte, Bruna Custódio Dias
Snail
Caramujo
UPS
Signaling pathway
Via de sinalização
Bioinformatics
Bioinformática
Schistosomiasis
Esquistossomose
CNPQ::CIENCIAS BIOLOGICAS::PARASITOLOGIA
title_short Genome-wide identification, characterization and expression profiling of the ubiquitin-proteasome genes in Biomphalaria glabrata
title_full Genome-wide identification, characterization and expression profiling of the ubiquitin-proteasome genes in Biomphalaria glabrata
title_fullStr Genome-wide identification, characterization and expression profiling of the ubiquitin-proteasome genes in Biomphalaria glabrata
title_full_unstemmed Genome-wide identification, characterization and expression profiling of the ubiquitin-proteasome genes in Biomphalaria glabrata
title_sort Genome-wide identification, characterization and expression profiling of the ubiquitin-proteasome genes in Biomphalaria glabrata
author Duarte, Bruna Custódio Dias
author_facet Duarte, Bruna Custódio Dias
author_role author
dc.contributor.none.fl_str_mv Gomes, Matheus de Souza
http://lattes.cnpq.br/8674610062329213
Barbosa, Aulus Estevão Anjos de Deus
http://lattes.cnpq.br/5331406701784638
Freitas, Guilherme Ramos Oliveira e
http://lattes.cnpq.br/4014066906990228
dc.contributor.author.fl_str_mv Duarte, Bruna Custódio Dias
dc.subject.por.fl_str_mv Snail
Caramujo
UPS
Signaling pathway
Via de sinalização
Bioinformatics
Bioinformática
Schistosomiasis
Esquistossomose
CNPQ::CIENCIAS BIOLOGICAS::PARASITOLOGIA
topic Snail
Caramujo
UPS
Signaling pathway
Via de sinalização
Bioinformatics
Bioinformática
Schistosomiasis
Esquistossomose
CNPQ::CIENCIAS BIOLOGICAS::PARASITOLOGIA
description Trabalho de Conclusão de Curso (Graduação)
publishDate 2019
dc.date.none.fl_str_mv 2019-12-18T19:12:34Z
2019-12-18T19:12:34Z
2019-12-11
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/bachelorThesis
format bachelorThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv DUARTE, Bruna Custódio Dias. Genome-wide identification, characterization and expression profiling of the ubiquitin-proteasome genes in Biomphalaria glabrata. 2019. 46 f. Trabalho de Conclusão de Curso (Graduação em Biotecnologia) - Universidade Federal de Uberlândia, Patos de Minas, 2019.
https://repositorio.ufu.br/handle/123456789/27815
identifier_str_mv DUARTE, Bruna Custódio Dias. Genome-wide identification, characterization and expression profiling of the ubiquitin-proteasome genes in Biomphalaria glabrata. 2019. 46 f. Trabalho de Conclusão de Curso (Graduação em Biotecnologia) - Universidade Federal de Uberlândia, Patos de Minas, 2019.
url https://repositorio.ufu.br/handle/123456789/27815
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/3.0/us/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/3.0/us/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Uberlândia
Brasil
Biotecnologia
publisher.none.fl_str_mv Universidade Federal de Uberlândia
Brasil
Biotecnologia
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFU
instname:Universidade Federal de Uberlândia (UFU)
instacron:UFU
instname_str Universidade Federal de Uberlândia (UFU)
instacron_str UFU
institution UFU
reponame_str Repositório Institucional da UFU
collection Repositório Institucional da UFU
repository.name.fl_str_mv Repositório Institucional da UFU - Universidade Federal de Uberlândia (UFU)
repository.mail.fl_str_mv diinf@dirbi.ufu.br
_version_ 1813711362627469312

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