Glucagon reduces airway hyperreactivity, inflammation, and remodeling induced by ovalbumin
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Outros Autores: | , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.1038/s41598-019-42981-6 |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/35315 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil. |
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Insuela, Daniella B. R.Azevedo, Carolina T.Coutinho, Diego S.Magalhães, Nathalia S.Ferrero, Maximiliano R.Ferreira, Tatiana Paula T.Cascabulho, Cynthia M.Henriques-Pons, AndreaOlsen, Priscilla C.Diaz, Bruno L.Silva, Patricia M. R.Cordeiro, Renato S. B.Martins, Marco A.Carvalho, Vinicius F.2019-09-03T15:24:55Z2019-09-03T15:24:55Z2019INSUELA, Daniella B. R. et al. Glucagon reduces airway hyperreactivity, inflammation, and remodeling induced by ovalbumin. Scientifc Reports, v. 9, n. 6478, p. 1-17, 2019.2045-2322https://www.arca.fiocruz.br/handle/icict/3531510.1038/s41598-019-42981-6engNature ResearchGlucagonRedução da hiper-reatividadeVias aéreasOvalbuminaInflamaçãoGlucagonAirway hyperreactivityReductionInflammationOvalbuminGlucagon reduces airway hyperreactivity, inflammation, and remodeling induced by ovalbumininfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Faculdade de Farmácia. Departamento de Análises Toxicológicas e Clínicas. Laboratório de Bacteriologia Clínica e Imunologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil.Glucagon has been shown to be beneficial as a treatment for bronchospasm in asthmatics. Here, we investigate if glucagon would prevent airway hyperreactivity (AHR), lung inflammation, and remodeling in a murine model of asthma. Glucagon (10 and 100 µg/Kg, i.n.) significantly prevented AHR and eosinophilia in BAL and peribronchiolar region induced by ovalbumin (OVA) challenge, while only the dose of 100 µg/Kg of glucagon inhibited subepithelial fibrosis and T lymphocytes accumulation in BAL and lung. The inhibitory action of glucagon occurred in parallel with reduction of OVA-induced generation of IL-4, IL-5, IL-13, TNF-α, eotaxin-1/CCL11, and eotaxin-2/CCL24 but not MDC/CCL22 and TARC/CCL17. The inhibitory effect of glucagon (100 µg/Kg, i.n.) on OVA-induced AHR and collagen deposition was reversed by pre-treatment with indomethacin (10 mg/Kg, i.p.). Glucagon increased intracellular cAMP levels and inhibits anti-CD3 plus anti-CD28-induced proliferation and production of IL-2, IL-4, IL-10, and TNF- α from TCD4+ cells in vitro. These findings suggest that glucagon reduces crucial features of asthma, including AHR, lung inflammation, and remodeling, in a mechanism probably associated with inhibition of eosinophils accumulation and TCD4+ cell proliferation and function. Glucagon should be further investigated as an option for asthma therapy.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/35315/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALDanielaInsuela_ViniciusCarvalho_etal_IOC_2019.pdfDanielaInsuela_ViniciusCarvalho_etal_IOC_2019.pdfapplication/pdf3642773https://www.arca.fiocruz.br/bitstream/icict/35315/2/DanielaInsuela_ViniciusCarvalho_etal_IOC_2019.pdfb3bdedb804a047d956503370fc78718eMD52TEXTDanielaInsuela_ViniciusCarvalho_etal_IOC_2019.pdf.txtDanielaInsuela_ViniciusCarvalho_etal_IOC_2019.pdf.txtExtracted texttext/plain74588https://www.arca.fiocruz.br/bitstream/icict/35315/3/DanielaInsuela_ViniciusCarvalho_etal_IOC_2019.pdf.txt0b66dd80d96f27297a7534d638584d44MD53icict/353152019-09-04 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| dc.title.pt_BR.fl_str_mv |
Glucagon reduces airway hyperreactivity, inflammation, and remodeling induced by ovalbumin |
| title |
Glucagon reduces airway hyperreactivity, inflammation, and remodeling induced by ovalbumin |
| spellingShingle |
Glucagon reduces airway hyperreactivity, inflammation, and remodeling induced by ovalbumin Insuela, Daniella B. R. Glucagon Redução da hiper-reatividade Vias aéreas Ovalbumina Inflamação Glucagon Airway hyperreactivity Reduction Inflammation Ovalbumin |
| title_short |
Glucagon reduces airway hyperreactivity, inflammation, and remodeling induced by ovalbumin |
| title_full |
Glucagon reduces airway hyperreactivity, inflammation, and remodeling induced by ovalbumin |
| title_fullStr |
Glucagon reduces airway hyperreactivity, inflammation, and remodeling induced by ovalbumin |
| title_full_unstemmed |
Glucagon reduces airway hyperreactivity, inflammation, and remodeling induced by ovalbumin |
| title_sort |
Glucagon reduces airway hyperreactivity, inflammation, and remodeling induced by ovalbumin |
| author |
Insuela, Daniella B. R. |
| author_facet |
Insuela, Daniella B. R. Azevedo, Carolina T. Coutinho, Diego S. Magalhães, Nathalia S. Ferrero, Maximiliano R. Ferreira, Tatiana Paula T. Cascabulho, Cynthia M. Henriques-Pons, Andrea Olsen, Priscilla C. Diaz, Bruno L. Silva, Patricia M. R. Cordeiro, Renato S. B. Martins, Marco A. Carvalho, Vinicius F. |
| author_role |
author |
| author2 |
Azevedo, Carolina T. Coutinho, Diego S. Magalhães, Nathalia S. Ferrero, Maximiliano R. Ferreira, Tatiana Paula T. Cascabulho, Cynthia M. Henriques-Pons, Andrea Olsen, Priscilla C. Diaz, Bruno L. Silva, Patricia M. R. Cordeiro, Renato S. B. Martins, Marco A. Carvalho, Vinicius F. |
| author2_role |
author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Insuela, Daniella B. R. Azevedo, Carolina T. Coutinho, Diego S. Magalhães, Nathalia S. Ferrero, Maximiliano R. Ferreira, Tatiana Paula T. Cascabulho, Cynthia M. Henriques-Pons, Andrea Olsen, Priscilla C. Diaz, Bruno L. Silva, Patricia M. R. Cordeiro, Renato S. B. Martins, Marco A. Carvalho, Vinicius F. |
| dc.subject.other.pt_BR.fl_str_mv |
Glucagon Redução da hiper-reatividade Vias aéreas Ovalbumina Inflamação |
| topic |
Glucagon Redução da hiper-reatividade Vias aéreas Ovalbumina Inflamação Glucagon Airway hyperreactivity Reduction Inflammation Ovalbumin |
| dc.subject.en.pt_BR.fl_str_mv |
Glucagon Airway hyperreactivity Reduction Inflammation Ovalbumin |
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Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil. |
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2019 |
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2019-09-03T15:24:55Z |
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2019-09-03T15:24:55Z |
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2019 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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INSUELA, Daniella B. R. et al. Glucagon reduces airway hyperreactivity, inflammation, and remodeling induced by ovalbumin. Scientifc Reports, v. 9, n. 6478, p. 1-17, 2019. |
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https://www.arca.fiocruz.br/handle/icict/35315 |
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2045-2322 |
| dc.identifier.doi.none.fl_str_mv |
10.1038/s41598-019-42981-6 |
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INSUELA, Daniella B. R. et al. Glucagon reduces airway hyperreactivity, inflammation, and remodeling induced by ovalbumin. Scientifc Reports, v. 9, n. 6478, p. 1-17, 2019. 2045-2322 10.1038/s41598-019-42981-6 |
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Nature Research |
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