Fish Oil Has Beneficial Effects on Allergen-Induced Airway Inflammation and Hyperreactivity in Mice

Detalhes bibliográficos
Autor(a) principal: Bargut, Thereza Cristina Lonzetti
Data de Publicação: 2013
Outros Autores: Ferreira, Tatiana Paula Teixeira, Daleprane, Julio Beltrame, Martins, Marco Aurélio, Silva, Patrícia Machado Rodrigues, Aguilla, Marcia Barbosa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/11748
Resumo: Universidade do Estado do Rio de Janeiro. Instituto de Biologia. Centro Biomédico. Laboratório de Morfometria, Metabolismo e Doença Cardiovascular. Rio de Janeiro, RJ, Brasil.
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spelling Bargut, Thereza Cristina LonzettiFerreira, Tatiana Paula TeixeiraDaleprane, Julio BeltrameMartins, Marco AurélioSilva, Patrícia Machado RodriguesAguilla, Marcia Barbosa2015-09-21T17:25:37Z2015-09-21T17:25:37Z2013BARGUT, Thereza Cristina Lonzetti; et al. Fish Oil Has Beneficial Effects on Allergen-Induced Airway Inflammation and Hyperreactivity in Mice. Plos One, v.8, n.9, 10p, Sept. 2013.https://www.arca.fiocruz.br/handle/icict/1174810.1371/journal.pone.0075059engPlos OneFish Oil Has Beneficial Effects on Allergen-Induced Airway Inflammation and Hyperreactivity in Miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade do Estado do Rio de Janeiro. Instituto de Biologia. Centro Biomédico. Laboratório de Morfometria, Metabolismo e Doença Cardiovascular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Instituto de Biologia. Centro Biomédico. Laboratório de Morfometria, Metabolismo e Doença Cardiovascular. Rio de Janeiro, RJ, Brasil.Background: Fish oil (FO) is rich in n-3 polyunsaturated fatty acids (PUFA), which have been suggested to be antiinflammatory and are associated with improvement of several inflammatory diseases. In this study, we investigated the influence of FO on allergen-induced lung inflammation and airway hyperreactivity in mice. Methods: Male A/J mice were fed either a standard-chow (SC) or a FO diet (FO) for 8 weeks. After 4 weeks, each group was further randomized for ovalbumin (SC-OVA and FO-OVA) or saline (SC-SAL and FO-SAL) challenge. Resistance and elastance were measured at baseline and after aerosolized methacholine, 24h after the last challenge. Bronchoalveolar lavage (BAL) was performed for leukocyte counts. Lung tissue mucus deposition, peribronchiolar matrix deposition and eosinophil infiltration were quantified. Serum immunoglobulin E (IgE) and IgG1 (ref 2.2), lung IL-4, IL-5, IL-10, IL-13, IL-17, INFγ and eotaxin-1 and 2 were detected by ELISA and nuclear factor kappa B (NFκB), GATA-3 and peroxisome proliferator-activated receptor gamma (PPARγ) expression was measured by Western blot. Results: Levels of serum IgE and IgG1 were significantly higher in OVA sensitized mice. OVA challenge resulted in increased eosinophil infiltration, increased inflammatory cytokine production, peribronchiolar matrix and mucus deposition and airway hyperreactivity to aerosolized methacholine. Elevated lung NFκB and GATA-3 expression was noted in OVA-challenged mice. These changes were attenuated in mice fed with FO diet. Higher PPARγ expression was also detected in the lungs from the FO-fed groups. Conclusion: Our results demonstrate that FO intake attenuated classical asthma features by suppressing the systemic sensitization, thus providing evidence that FO might be a prophylactic alternative for asthma prevention.MiceAllergen-Induced Airway InflammationFish OilHyperreactivityAsthmaDessensibilização ImunológicaHiper-ReatividadeCamundongosInflamaçãoAsmainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/11748/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALjulio_daleprane_etal_IOC_2013.pdfapplication/pdf1568849https://www.arca.fiocruz.br/bitstream/icict/11748/2/julio_daleprane_etal_IOC_2013.pdfe58c5bac8f67310595867dae20041083MD52TEXTjulio_daleprane_etal_IOC_2013.pdf.txtjulio_daleprane_etal_IOC_2013.pdf.txtExtracted texttext/plain52101https://www.arca.fiocruz.br/bitstream/icict/11748/3/julio_daleprane_etal_IOC_2013.pdf.txt4f86b11829d9baf1ac45f6d9d578553fMD53icict/117482022-06-24 12:17:37.517oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T15:17:37Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Fish Oil Has Beneficial Effects on Allergen-Induced Airway Inflammation and Hyperreactivity in Mice
title Fish Oil Has Beneficial Effects on Allergen-Induced Airway Inflammation and Hyperreactivity in Mice
spellingShingle Fish Oil Has Beneficial Effects on Allergen-Induced Airway Inflammation and Hyperreactivity in Mice
Bargut, Thereza Cristina Lonzetti
Mice
Allergen-Induced Airway Inflammation
Fish Oil
Hyperreactivity
Asthma
Dessensibilização Imunológica
Hiper-Reatividade
Camundongos
Inflamação
Asma
title_short Fish Oil Has Beneficial Effects on Allergen-Induced Airway Inflammation and Hyperreactivity in Mice
title_full Fish Oil Has Beneficial Effects on Allergen-Induced Airway Inflammation and Hyperreactivity in Mice
title_fullStr Fish Oil Has Beneficial Effects on Allergen-Induced Airway Inflammation and Hyperreactivity in Mice
title_full_unstemmed Fish Oil Has Beneficial Effects on Allergen-Induced Airway Inflammation and Hyperreactivity in Mice
title_sort Fish Oil Has Beneficial Effects on Allergen-Induced Airway Inflammation and Hyperreactivity in Mice
author Bargut, Thereza Cristina Lonzetti
author_facet Bargut, Thereza Cristina Lonzetti
Ferreira, Tatiana Paula Teixeira
Daleprane, Julio Beltrame
Martins, Marco Aurélio
Silva, Patrícia Machado Rodrigues
Aguilla, Marcia Barbosa
author_role author
author2 Ferreira, Tatiana Paula Teixeira
Daleprane, Julio Beltrame
Martins, Marco Aurélio
Silva, Patrícia Machado Rodrigues
Aguilla, Marcia Barbosa
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Bargut, Thereza Cristina Lonzetti
Ferreira, Tatiana Paula Teixeira
Daleprane, Julio Beltrame
Martins, Marco Aurélio
Silva, Patrícia Machado Rodrigues
Aguilla, Marcia Barbosa
dc.subject.en.pt_BR.fl_str_mv Mice
Allergen-Induced Airway Inflammation
Fish Oil
Hyperreactivity
Asthma
topic Mice
Allergen-Induced Airway Inflammation
Fish Oil
Hyperreactivity
Asthma
Dessensibilização Imunológica
Hiper-Reatividade
Camundongos
Inflamação
Asma
dc.subject.decs.pt_BR.fl_str_mv Dessensibilização Imunológica
Hiper-Reatividade
Camundongos
Inflamação
Asma
description Universidade do Estado do Rio de Janeiro. Instituto de Biologia. Centro Biomédico. Laboratório de Morfometria, Metabolismo e Doença Cardiovascular. Rio de Janeiro, RJ, Brasil.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2015-09-21T17:25:37Z
dc.date.available.fl_str_mv 2015-09-21T17:25:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BARGUT, Thereza Cristina Lonzetti; et al. Fish Oil Has Beneficial Effects on Allergen-Induced Airway Inflammation and Hyperreactivity in Mice. Plos One, v.8, n.9, 10p, Sept. 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/11748
dc.identifier.doi.pt_BR.fl_str_mv 10.1371/journal.pone.0075059
identifier_str_mv BARGUT, Thereza Cristina Lonzetti; et al. Fish Oil Has Beneficial Effects on Allergen-Induced Airway Inflammation and Hyperreactivity in Mice. Plos One, v.8, n.9, 10p, Sept. 2013.
10.1371/journal.pone.0075059
url https://www.arca.fiocruz.br/handle/icict/11748
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Plos One
publisher.none.fl_str_mv Plos One
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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