Novel mutations associated with resistance to Bacillus sphaericus in a polymorphic region of the Culex quinquefasciatus cqm1 gene

Detalhes bibliográficos
Autor(a) principal: Chalegre, Karlos Diogo de Melo
Data de Publicação: 2012
Outros Autores: Romão, Tatiany Patrícia, Tavares, Daniella Aliny, Santos, Eloína Mendonça, Ferreira, Lígia Maria, Oliveira, Cláudia Maria Fontes, de-Melo-Neto, Osvaldo Pompílio, Silva-Filha, Maria Helena Neves Lobo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/25706
Resumo: Este trabalho foi apoiado pelo Programa Estratégico de Apoio à Pesquisa em Saúde (PAPES) da Fundação Oswaldo Cruz - FIOCRUZ, Conselho Nacional de Pesquisa (CNPq Brasil, bolsa 403488 / 2008-7 ;), Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE Brasil, outorga APQ 0427-2.13 / 08 ).
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spelling Chalegre, Karlos Diogo de MeloRomão, Tatiany PatríciaTavares, Daniella AlinySantos, Eloína MendonçaFerreira, Lígia MariaOliveira, Cláudia Maria Fontesde-Melo-Neto, Osvaldo PompílioSilva-Filha, Maria Helena Neves Lobo2018-04-10T12:06:16Z2018-04-10T12:06:16Z2012CHALEGRE, K. D. DE M. et al. Novel mutations associated with resistance to Bacillus sphaericus in a polymorphic region of the Culex quinquefasciatus cqm1 gene. Applied and Environmental Microbiology, v. 78, n. 17, p. 6321–6326, set. 2012.1098-5336https://www.arca.fiocruz.br/handle/icict/2570610.1128/AEM.01199-12Este trabalho foi apoiado pelo Programa Estratégico de Apoio à Pesquisa em Saúde (PAPES) da Fundação Oswaldo Cruz - FIOCRUZ, Conselho Nacional de Pesquisa (CNPq Brasil, bolsa 403488 / 2008-7 ;), Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE Brasil, outorga APQ 0427-2.13 / 08 ).Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.Bin toxin from Bacillus sphaericus acts on Culex quinquefasciatus larvae by binding to Cqm1 midgut-bound receptors, and disruption of the cqm1 gene is the major cause of resistance. The goal of this work was to screen for a laboratory-selected resistance cqm1(REC) allele in field populations in the city of Recife, Brazil, and to describe other resistance-associated polymorphisms in the cqm1 gene. The cqm1(REC) allele was detected in the four nontreated populations surveyed at frequencies from 0.001 to 0.017, and sequence analysis from these samples revealed a novel resistant allele (cqm1(REC-D16)) displaying a 16-nucletotide (nt) deletion which is distinct from the 19-nt deletion associated with cqm1(REC). Yet a third resistant allele (cqm1(REC-D25)), displaying a 25-nt deletion, was identified in samples from a treated area exposed to B. sphaericus. A comparison of the three deletion events revealed that all are located within the same 208-nt region amplified during the screening procedure. They also introduce equivalent frameshifts in the sequence and generate the same premature stop codon, leading to putative transcripts encoding truncated proteins which are unable to locate to the midgut epithelium. The populations analyzed in this study contained a variety of alleles with mutations disrupting the function of the corresponding Bin toxin receptor. Their locations reveal a hot spot that can be exploited to assess the resistance risk through DNA screening.engResistênciaEpidemiologiaMosquitoDNAResistanceEpidemiologyMosquitoDNAAlelosAnimaisBacilo / patogenicidadeToxinas bacterianas / toxicidadeBrasilCulex / genéticaCulex / imunologiaCulex / microbiologiaProteínas de Insetos / genéticaMutaçãoPolimorfismo GenéticoReceptores da Superfície Celular / genéticaNovel mutations associated with resistance to Bacillus sphaericus in a polymorphic region of the Culex quinquefasciatus cqm1 geneinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83092https://www.arca.fiocruz.br/bitstream/icict/25706/1/license.txt447f2497af1ef5cf99b5c07f6fa18dceMD51ORIGINALNovel Mutations Associated with Resistance to Bacillus sphaericus in a.pdfNovel Mutations Associated with Resistance to Bacillus sphaericus in a.pdfapplication/pdf338176https://www.arca.fiocruz.br/bitstream/icict/25706/2/Novel%20Mutations%20Associated%20with%20Resistance%20to%20Bacillus%20sphaericus%20in%20a.pdf9e301f894bd190598a46f77cc5bdebc7MD52TEXTNovel Mutations Associated with Resistance to Bacillus sphaericus in a.pdf.txtNovel Mutations Associated with Resistance to Bacillus sphaericus in a.pdf.txtExtracted texttext/plain40816https://www.arca.fiocruz.br/bitstream/icict/25706/3/Novel%20Mutations%20Associated%20with%20Resistance%20to%20Bacillus%20sphaericus%20in%20a.pdf.txt87dea6a9466b987b2926b3a711566494MD53icict/257062018-08-15 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dc.title.pt_BR.fl_str_mv Novel mutations associated with resistance to Bacillus sphaericus in a polymorphic region of the Culex quinquefasciatus cqm1 gene
title Novel mutations associated with resistance to Bacillus sphaericus in a polymorphic region of the Culex quinquefasciatus cqm1 gene
spellingShingle Novel mutations associated with resistance to Bacillus sphaericus in a polymorphic region of the Culex quinquefasciatus cqm1 gene
Chalegre, Karlos Diogo de Melo
Resistência
Epidemiologia
Mosquito
DNA
Resistance
Epidemiology
Mosquito
DNA
Alelos
Animais
Bacilo / patogenicidade
Toxinas bacterianas / toxicidade
Brasil
Culex / genética
Culex / imunologia
Culex / microbiologia
Proteínas de Insetos / genética
Mutação
Polimorfismo Genético
Receptores da Superfície Celular / genética
title_short Novel mutations associated with resistance to Bacillus sphaericus in a polymorphic region of the Culex quinquefasciatus cqm1 gene
title_full Novel mutations associated with resistance to Bacillus sphaericus in a polymorphic region of the Culex quinquefasciatus cqm1 gene
title_fullStr Novel mutations associated with resistance to Bacillus sphaericus in a polymorphic region of the Culex quinquefasciatus cqm1 gene
title_full_unstemmed Novel mutations associated with resistance to Bacillus sphaericus in a polymorphic region of the Culex quinquefasciatus cqm1 gene
title_sort Novel mutations associated with resistance to Bacillus sphaericus in a polymorphic region of the Culex quinquefasciatus cqm1 gene
author Chalegre, Karlos Diogo de Melo
author_facet Chalegre, Karlos Diogo de Melo
Romão, Tatiany Patrícia
Tavares, Daniella Aliny
Santos, Eloína Mendonça
Ferreira, Lígia Maria
Oliveira, Cláudia Maria Fontes
de-Melo-Neto, Osvaldo Pompílio
Silva-Filha, Maria Helena Neves Lobo
author_role author
author2 Romão, Tatiany Patrícia
Tavares, Daniella Aliny
Santos, Eloína Mendonça
Ferreira, Lígia Maria
Oliveira, Cláudia Maria Fontes
de-Melo-Neto, Osvaldo Pompílio
Silva-Filha, Maria Helena Neves Lobo
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Chalegre, Karlos Diogo de Melo
Romão, Tatiany Patrícia
Tavares, Daniella Aliny
Santos, Eloína Mendonça
Ferreira, Lígia Maria
Oliveira, Cláudia Maria Fontes
de-Melo-Neto, Osvaldo Pompílio
Silva-Filha, Maria Helena Neves Lobo
dc.subject.other.pt_BR.fl_str_mv Resistência
Epidemiologia
Mosquito
DNA
topic Resistência
Epidemiologia
Mosquito
DNA
Resistance
Epidemiology
Mosquito
DNA
Alelos
Animais
Bacilo / patogenicidade
Toxinas bacterianas / toxicidade
Brasil
Culex / genética
Culex / imunologia
Culex / microbiologia
Proteínas de Insetos / genética
Mutação
Polimorfismo Genético
Receptores da Superfície Celular / genética
dc.subject.en.pt_BR.fl_str_mv Resistance
Epidemiology
Mosquito
DNA
dc.subject.decs.pt_BR.fl_str_mv Alelos
Animais
Bacilo / patogenicidade
Toxinas bacterianas / toxicidade
Brasil
Culex / genética
Culex / imunologia
Culex / microbiologia
Proteínas de Insetos / genética
Mutação
Polimorfismo Genético
Receptores da Superfície Celular / genética
description Este trabalho foi apoiado pelo Programa Estratégico de Apoio à Pesquisa em Saúde (PAPES) da Fundação Oswaldo Cruz - FIOCRUZ, Conselho Nacional de Pesquisa (CNPq Brasil, bolsa 403488 / 2008-7 ;), Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE Brasil, outorga APQ 0427-2.13 / 08 ).
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2018-04-10T12:06:16Z
dc.date.available.fl_str_mv 2018-04-10T12:06:16Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CHALEGRE, K. D. DE M. et al. Novel mutations associated with resistance to Bacillus sphaericus in a polymorphic region of the Culex quinquefasciatus cqm1 gene. Applied and Environmental Microbiology, v. 78, n. 17, p. 6321–6326, set. 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/25706
dc.identifier.issn.pt_BR.fl_str_mv 1098-5336
dc.identifier.doi.none.fl_str_mv 10.1128/AEM.01199-12
identifier_str_mv CHALEGRE, K. D. DE M. et al. Novel mutations associated with resistance to Bacillus sphaericus in a polymorphic region of the Culex quinquefasciatus cqm1 gene. Applied and Environmental Microbiology, v. 78, n. 17, p. 6321–6326, set. 2012.
1098-5336
10.1128/AEM.01199-12
url https://www.arca.fiocruz.br/handle/icict/25706
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/25706/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/25706/2/Novel%20Mutations%20Associated%20with%20Resistance%20to%20Bacillus%20sphaericus%20in%20a.pdf
https://www.arca.fiocruz.br/bitstream/icict/25706/3/Novel%20Mutations%20Associated%20with%20Resistance%20to%20Bacillus%20sphaericus%20in%20a.pdf.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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